We narrowed to 9,597 results for: EPO
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Plasmid#103275PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-181a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-181a-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-181b-2-3p
Plasmid#103277PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-181b-2-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-181b-2-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-133a-5p
Plasmid#103222PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-133a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-133a-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-135a-3p
Plasmid#103226PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-135a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-135a-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1285-3p
Plasmid#103201PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1285-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-1285-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1285-5p
Plasmid#103202PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1285-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-1285-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-129-5p
Plasmid#103207PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-129-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-129-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1185-5p
Plasmid#103182PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1185-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-1185-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-125b-2-3p
Plasmid#103190PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-125b-2-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-125b-2-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-105-3p
Plasmid#103169PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-105-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-105-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-3130-3p
Plasmid#103422PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-3130-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-3130-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-519a-5p
Plasmid#103607PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-519a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-519a-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-509-5p
Plasmid#103591PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-509-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-509-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-135a-5p
Plasmid#103227PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-135a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-135a-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1
Plasmid#103160PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-1 target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFL-SV40-TAK1 5'-UTR
Plasmid#115356Purposefirefly luciferase (Fluc) reporter containing the 5’-UTR of TAK1 mRNADepositorInsertTAK1 5'-UTR (MAP3K7 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAK4-FL-3'UTR-MT2
Plasmid#113093PurposeLuciferase reporter containing PAK4-FL- 3' UTR with the second predicted MiR-199a-3p binding site mutatedDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseTagsExpressionMammalianMutationSecond predicted miR-199a-3p binding site mutated…PromoterPGKAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAK4-FL-3'UTR-MT-both
Plasmid#113094PurposeLuciferase reporter containing PAK4-FL- 3' UTR with both the predicted MiR-199a-3p binding sites mutatedDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseTagsExpressionMammalianMutationBoth the predicted miR-199a-3p binding site muta…PromoterPGKAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-I0X7Z6
Plasmid#103114PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertI0X7Z6(Cas9 coding gene from Treponema sp. JC4 GN=MSI_17100)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSi-check2-hRac2 3'UTR
Plasmid#97160PurposeFor dual luciferase report assayDepositorInsertRAC 3'UTR (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEMS2047
Plasmid#79655PurposessAAV genome with Ple258 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertssAAV Ple258-EmGFP WPRE
UseAAVTagsExpressionMutationPromoterPAX6 EnhancerAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
exon K-renilla
Plasmid#81007Purposeluciferase based mini-gene to report splicing at exon 25 in Drosophila paraylticDepositorInsertparalytic (para Fly)
UseTagsRenilla luciferaseExpressionInsectMutationspanning exon 24 to exon 26, termination codon in…PromoterP-AC5 (Actin 5C)Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
1.2 hA33 luciferase
Plasmid#68149Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -1149 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.6 hA33 luciferase
Plasmid#68150Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -595 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.44 hA33 luciferase
Plasmid#68151Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -443 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.42 hA33 luciferase
Plasmid#68152Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -423 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.41 hA33 luciferase
Plasmid#68154Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -405 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.36 hA33 luciferase
Plasmid#68155Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -363 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.38 hA33 luciferase
Plasmid#68156Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -384 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
0.22 hA33 luciferase
Plasmid#68157Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -222 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-mEKLF Zinc Fingers
Plasmid#67837PurposeExpression of murine EKLF Zinc Fingers (aa 287-end) fused to Flag and driven by SV40 promoterDepositorInsertEKLF (Klf1 Mouse)
UseTagsFlagExpressionMammalianMutationZinc Fingers are amino acids 287-end; this number…PromoterSV40Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL2-HKII-B
Plasmid#64514PurposeAS-30D hepatoma hexokinase II 3.0 kbp promoter-luciferase reporter vectorDepositorInsertType II hexokinase gene, partial cds and promoter region (Hk2 Rat)
UseLuciferaseTagsfirefly luciferaseExpressionMutationIsolated from AS-30D rat hepatomaPromoterHexokinase IIAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL2-HKII-F
Plasmid#64518PurposeAS-30D hepatoma hexokinase II 0.075 kbp exon I-luciferase reporter vectorDepositorInsertType II hexokinase gene, partial cds and promoter region (Hk2 Rat)
UseLuciferaseTagsfirefly luciferaseExpressionMutationIsolated from AS-30D rat hepatomaPromoterHexokinase II promoter deletedAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP
Plasmid#58490PurposeExpresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltageDepositorInsertArchaerhodopsin-3 w/ D95H point mutation fused to eGFP
UseLentiviralTagseGFPExpressionMammalianMutationChanged Aspartic Acid 95 to GlutaminePromoterUbiquitinAvailable sinceAug. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEMS1207
Plasmid#29104PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorInsertPle235
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES
Plasmid#138299PurposeEncodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertmCherry-PKCsub(T/A)-RLuc8N-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
G-GECO1.2-Orai1
Plasmid#73562PurposeGreen fluorescent reporter of Orai1-associated calcium influxDepositorInsertG-GECO1.2-Orai1 (ORAI1 Synthetic, Human)
UseTagsG-GECO1.2ExpressionMammalianMutationPromoterCMV Immediate EarlyAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Blast DEST JNKKTRmRuby2
Plasmid#59154PurposeLentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)DepositorInsertJNK Kinase Translocation Reporter (MAPK8 Human)
UseLentiviralTagsmRuby2ExpressionMammalianMutationPromoterPGKAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKeima-LiveDrop
Plasmid#199695Purposelipophagy reporter system based on the Keima-fluorophoreDepositorInsertGPAT4 (GPAT4 Human)
UseTagsKeimaExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRPML1-G-GECO1.2-ERES
Plasmid#207144PurposeFusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signalDepositorInsertMcoln1 (MCOLN1 Human)
UseTagsER-Export Signal (ERES) and G-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.27_ARE/NRF2-SPE
Plasmid#177775PurposeLuciferase reporter for ARE/NRF2DepositorInsertNRF2 (NFE2L2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRLsin-SV40 T antigen-IRES-mCherry
Plasmid#58993Purposelentiviral expression of SV40 large T antigen and mCherry red fluorescent reporterDepositorInsertsCMV enhancer-chicken beta actin promoter
SV40 large T antigen
IRES-mCherry red fluorescent protein
UseLentiviralTagsExpressionMammalianMutationEcoRV site in MCS converted to BamHI sitePromoterCMV enhancer-chicken beta actinAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Gal3
Plasmid#85662PurposeFluorescent reporter for lysosomal damageDepositorInsertLGALS3 (LGALS3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA
Plasmid#119946PurposeExpresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorInsertRenilla luciferase
UseTags24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …ExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZsGreen1-cMYC/pLVX-Puromycin
Plasmid#180278PurposePlasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).DepositorInsertZsGreen1-cMYC fusion protein MYC (MYC Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-hygro-STING
Plasmid#102598PurposeExpress STING for IFN-beta luciferase reporter assay.DepositorInsertSTING (STING1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.0-TERT WT
Plasmid#84924Purposeluciferase reporter for TERT promoterDepositorInsertTERT promoter (TERT Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterTERTAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
Artichoke
Plasmid#73320PurposeArtichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationPromoterSFFV and EF1aAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-XBP1 mNeonGreen NLS
Plasmid#115971PurposeER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen (retroviral vector backbone)DepositorInsertX-box binding protein 1 (XBP1 Synthetic, Human)
UseRetroviralTagsHA tag, c-myc NLS, and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_071
Plasmid#164558PurposeHigher titer version of pXPR_053. Lentiviral vector that enables constitutive sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.DepositorInsertsgRNA cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only