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8,883 results

Showing: 8001 - 8050 of 8883 results

  5. MSP2443

    Plasmid
    #72252
    Purpose
    Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72252-plasmid-map-sequence-id-207329

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  7. pGEX-2T-RhoA-wt

    Plasmid
    #12959
    Depositor
    Gary Bokoch
    Insert
    RhoA (RHOA Human)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    March 2, 2007
    Availability
    Academic Institutions and Nonprofits only

  14. pCMVHA-E2F5

    Plasmid
    #24213
    Purpose
    Mammalian expression of human E2F5 with HA tag
    Depositor
    Kristian Helin
    Insert
    E2F5 (E2F5 Human)
    Tags
    HA
    Expression
    Mammalian
    Available Since
    Dec. 15, 2010
    Availability
    Academic Institutions and Nonprofits only
    24213-plasmid-map-sequence-id-245679

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  26. CMV-EGFP-ssHRP-TM

    Plasmid
    #31156
    Depositor
    Hollis Cline
    Article
    Li et al Front Neural Circuits. 2010 Feb 26;4:6.
    Insert
    ssHRP-TM
    Use
    Membrane targeted hrp (mhrp)
    Expression
    Mammalian
    Mutation
    includes signal sequence and a transmembrane doma…
    Promoter
    CMV promoter including immediate early promoter/e…
    Available Since
    Dec. 14, 2011
    Availability
    Academic Institutions and Nonprofits only

  28. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE

    Plasmid
    #104492
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9
    Insert
    jGCaMP7f
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104492-plasmid-map-sequence-id-381181

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  33. pCDNA3-LOV-VP16

    Plasmid
    #42499
    Purpose
    LOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factor
    Depositor
    Ricardo Dolmetsch
    Article
    Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d
    Insert
    FKF1 (N-LOV, G128D mutant) fused with VP16
    Tags
    F-type VP16 activation domain and HA tag
    Expression
    Mammalian
    Mutation
    G128D 1-168aa
    Promoter
    CMV
    Available Since
    March 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    42499-plasmid-map-sequence-id-57336

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  34. pRK5-myc-Rac1-wt

    Plasmid
    #12985
    Depositor
    Gary Bokoch
    Insert
    Rac1 (RAC1 Human)
    Tags
    Myc
    Expression
    Mammalian
    Available Since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
    12985-plasmid-map-sequence-id-217442

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  36. FCK-Halo-GFP

    Plasmid
    #14750
    Purpose
    Lentiviral expression of Halo-GFP for neuronal inhibition
    Depositor
    Edward Boyden
    Article
    Han et al PLoS One. 2007 Mar 21;2(3):e299.
    Insert
    Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    N. pharaonis halorhodopsin is mammalian codon-opt…
    Promoter
    CaMKIIa
    Available Since
    April 5, 2007
    Availability
    Academic Institutions and Nonprofits only
    14750-plasmid-map-sequence-id-192940

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  43. pGEX-2T-Rac1-Q61L

    Plasmid
    #12979
    Depositor
    Gary Bokoch
    Insert
    Rac1 constitutively active (RAC1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Q61L Constitutively active
    Available Since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only

  44. CMV-O-GECO1

    Plasmid
    #46025
    Purpose
    Orange intensiometric genetically encoded Ca2+-indicators for optical imaging
    Depositor
    Robert Campbell
    Article
    Wu et al ACS Chem Neurosci. 2013 Mar 19.
    Insert
    O-GECO1
    Expression
    Mammalian
    Mutation
    R-GECO1 N45I/A73V/S142P/M146R/M223T/M339L/K378R/E…
    Promoter
    CMV
    Available Since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    46025-plasmid-map-sequence-id-311519

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Showing: 8001 - 8050 of 8883 results