We narrowed to 13,043 results for: SHI
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pXylA-RgTALsyn
Plasmid#100948PurposeExpression of Tyrosine Ammonia Lyase from constitutive XylA promoterDepositorInsertRgTALsyn
UseSynthetic BiologyTagsExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterXylA (Constitutive)Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GW-V5-Hygro
Plasmid#68265PurposeConstitutive selectable expression of C-term V5 tagged proteinsDepositorTypeEmpty backboneUseTagsV5ExpressionMammalianMutationPromoterCAGAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2
Plasmid#91873PurposePhoto-activatable/light sensitive Vav2DepositorInsertsUseTagsmCherryExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat4-SH2
Plasmid#46514DepositorInsertStat4 (STAT4 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 133-678PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Slap-SH2
Plasmid#46500DepositorInsertSlap (SLA Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 61-221PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syk(C)-SH2
Plasmid#46519DepositorInsertSyk(C) (SYK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 149-263PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2241 - [1-2] ENTR - Fluor - ce-mCardinal (1 intron, syntrons(3), no_atg, no_stop)
Plasmid#159860PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-mCardinal (1 intron, syntrons(3), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-EAF pBacPAK8
Plasmid#15323DepositorInsertELL Associated Factor (EAF1 Human)
UseBaculovirus constructionTags6x His and FLAGExpressionMutationPromoterpolyhedrinAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX Vav1-SH2
Plasmid#46528DepositorInsertVav1 (VAV1 Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 661-777PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSM32.2
Plasmid#101635PurposeEncodes the fluorescent protein Rosmarinus under a CMV promoter.DepositorInsertRosmarinus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
FnoCas12a-DR-cr in PBCSK-
Plasmid#126642PurposeCloning vector FnoCas12a DR-crRNA for expression in mammalian cellsDepositorInsertFnoCas12a Full Length Direct Repeat crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMDJ15 - pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]
Plasmid#159801PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
GluR2_adRNA(2,100,50)-GFP
Plasmid#124622PurposeExpresses adRNA(2,100,50) targeting the RAB7A transcript along with GFPDepositorInsertGluR2_adRNA(2,100,50)
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
GluR2_adRNA(0,100,50)-GFP
Plasmid#124623PurposeExpresses adRNA(0,100,50) targeting the RAB7A transcript along with GFPDepositorInsertGluR2_adRNA(0,100,50)
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-ELL2 pBacPAK8
Plasmid#15327DepositorInsertEleven Nineteen Lysine Rich in Leukemia 2 (ELL2 Human)
UseBaculovirus constructionTagsFLAGExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
mr in pUASP
Plasmid#112981PurposeP element vector for transposon insertion of mr (APC2) into Drosophila genomeDepositorInsertmr (APC2) (mr Fly)
UseP insertion vectorTagsExpressionMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
gRD21A-mRFP
Plasmid#181959PurposeRD21A protein tagged with mRFP, under control of native promoterDepositorInsertRD21A genomic sequence including 2kb upstream of the ATG
UseTagsmRFPExpressionPlantMutationPromoterRD21A nativeAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX Eat2-SH2
Plasmid#46423DepositorInsertEat2 (SH2D1B Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-132PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-GEX-GECO1
Plasmid#32464DepositorInsertGEX-GECO1.0
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…PromoterAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2210 - [1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)
Plasmid#159864PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 P139A (#625)
Plasmid#99945Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationP139APromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-eSpCas9
Plasmid#133370PurposePiggybac vector for constitutive expressionDepositorInserteSpCas9
UseTagsFlagExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 S137A (#627)
Plasmid#99943Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationS137APromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-Cx35-Halo-C-in
Plasmid#71511PurposeMammalian expression plasmid encoding perch Cx35 with an internal HaloTag between amino acids 285 and 286 in the C-terminus.DepositorInsertConnexin 35
UseTagsHaloTag (internal to sequence)ExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-RIPK3 K55R
Plasmid#78807PurposeTo overexpress RIPK3 K55R in Mammalian CellsDepositorInsertRIPK3 (RIPK3 Human)
UseTagsHAExpressionMammalianMutationK55RPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB076i6
Plasmid#79200PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (no operator) gIII, luxAB; Ppro1 434cI-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (no operator); Ppro1Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-EAF2 pBacPAK8
Plasmid#15326DepositorInsertELL Associated Factor 2 (EAF2 Human)
UseBaculovirus constructionTagsFLAGExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-RIPK3 S199A
Plasmid#78806PurposeTo overexpress RIPK3 S199A in Mammalian CellsDepositorInsertRIPK3 (RIPK3 Human)
UseTagsHAExpressionMammalianMutationS199APromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti CRISPR sgSHMT1_2
Plasmid#106312PurposeExpress Cas9 and sgRNA targeting SHMT1DepositorInsertsgRNA targeting SHMT1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSM14
Plasmid#101645PurposeEncodes the fluorescent protein efasCFP for constitutive expression in E. coli.DepositorInsertefasCFP
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pILGFP86
Plasmid#83543PurposeUsed to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.DepositorInsertRPL4A promoter-yEGFP
UseTagsExpressionYeastMutationPromoterRPL4AAvailable sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-Tubulin-6
Plasmid#55512PurposeLocalization: Microtubules, Excitation: 462, Emission: 492DepositorInsertTubulin (TUBA1B Human)
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX PLCg1(N)-SH2
Plasmid#46470DepositorInsertPLCg1(N)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 539-664PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorT(sp)
Plasmid#111255PurposeBroad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorT signal peptideDepositorInsertTorT signal peptide
UseTagsExpressionBacterialMutationPromoterPcAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
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MS2_adRNA-MCP_ADAR1 DD_NLS
Plasmid#124625PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD_NLSDepositorInsertMCP_ADAR1 DD_NLS
UseAAVTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCIneo-EloC
Plasmid#31982DepositorInsertElongin C (ELOC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHL 651
Plasmid#53016PurposeLacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997).DepositorInsertsLacIq
tetR
UseSynthetic BiologyTagsRBS(st3) and T1 terminatorExpressionBacterialMutationPromoterpConAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL T157I-pBabe-puro
Plasmid#19240DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Threonin 157 to IsoleucinPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL A149T-pBabe-puro
Plasmid#19242DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Alanin 149 to ThreoninPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
Plasmid#159802PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human T (BRACHYURY) sgRNA
Plasmid#59726PurposePlasmid encoding sgRNA to generate T (BRACHYURY) knock-out mutant human cellsDepositorInserthT sgRNA (TBXT Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1277
Plasmid#53017PurposeAmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) mCherry::Asp terminator + ColE1DepositorInsertsGFP
mCherry
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pMpGWB106-OEP7(1–50)
Plasmid#100602PurposeBinary vector for expression of Citrine-fused AtOEP7(1-50) (OEP7(1–50)-Citrine) to visualize chloroplast outer envelope membrane in plantsDepositorInsertOEP7(1–50)-Citrine (OEP7 Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
