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We narrowed to 18,759 results for: nls

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Showing: 6641 - 6680 of 18759 results

  6. pX335-EN477

    Plasmid
    #86232
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.
    Depositor
    Benoit Bruneau
    Publication
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86232-plasmid-map-sequence-id-177242

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  14. pMZ376

    Plasmid
    #74213
    Purpose
    Combined sgRNA/Cas9 vector with KanMX marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74213-plasmid-map-sequence-id-139302

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  15. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid
    #71828
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BACH2-sgRNA8 (BACH2 Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71828-plasmid-map-sequence-id-131023

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  19. pEF_dCas9-VP64

    Plasmid
    #68417
    Purpose
    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG and VP64
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68417-plasmid-map-sequence-id-120195

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  25. pX330 Ctnnb1.2

    Plasmid
    #59912
    Purpose
    pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter
    Depositor
    Tyler Jacks
    Publication
    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ctnnb1 (Ctnnb1 Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    59912-plasmid-map-sequence-id-123317

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  27. pGLTR-X-PURO

    Plasmid
    #58246
    Purpose
    GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selection
    Depositor
    Stephan Geley
    Publication
    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:
    Insert
    Puro(R)
    Use
    Lentiviral and RNAi; Gateway destionation vector
    Tags
    TetR-NLS-T2A
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58246-plasmid-map-sequence-id-89671

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  29. pLSLZ.D.R

    Plasmid
    #51510
    Purpose
    Single component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.
    Depositor
    Daniel Voytas
    Publication
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    Zif268:FokI
    us:NPTII repair template
    Use
    Plant t-dna plasmid
    Tags
    NLS
    Promoter
    2x35S
    Available since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51510-plasmid-map-sequence-id-78634

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  30. pLSLGFP

    Plasmid
    #51494
    Purpose
    pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Publication
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GFP
    us:NPTII repair template
    Use
    Plant t-dna plasmid
    Tags
    NLS and flag
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51494-plasmid-map-sequence-id-78590

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  36. pEMS1199

    Plasmid
    #29100
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple53 (DCX Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available since
    Nov. 22, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29100-plasmid-map-sequence-id-13515

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  40. TK4_SH4-1_targeting

    Plasmid
    #254452
    Purpose
    TK4 targeting vector containing left and right homology arms for the SH4-1 locus
    Depositor
    Marius Wernig
    Publication
    Uenaka et al Cell Stem Cell. 2026 Feb 13:S1934-5909(2
    Inserts
    mycNLS-mScarlet-T2A-mNeonGreen-PEST
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Piggybac
    Tags
    PEST, T2A-mycNLS-mTagBFP2, and c-myc-NLS
    Expression
    Mammalian
    Promoter
    CAG and EF1a
    Available since
    April 24, 2026
    Availability
    Academic Institutions and Nonprofits only
    254452-plasmid-map-sequence-id-507707

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Showing: 6641 - 6680 of 18759 results