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8,964 results

Showing: 8301 - 8350 of 8964 results

  5. MSP680

    Plasmid
    #65772
    Purpose
    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65772-plasmid-map-sequence-id-112654

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  8. pCRISPomyces-1

    Plasmid
    #61736
    Purpose
    Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA
    Depositor
    Huimin Zhao
    Article
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    tracrRNA
    crRNA cassette
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI
    Available Since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    61736-plasmid-map-sequence-id-256988

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  10. pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP

    Plasmid
    #35499
    Purpose
    AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.
    Depositor
    Karl Deisseroth
    Article
    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi
    Has Service
    AAV9
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    CaMKIIa
    Available Since
    April 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    35499-plasmid-map-sequence-id-199323

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  18. pBad-mAmetrine

    Plasmid
    #18083
    Purpose
    Bacterial expression of mAmetrine fluorescent protein
    Depositor
    Robert Campbell
    Article
    Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1
    Insert
    mAmetrine fluorescent proteins
    Tags
    His
    Expression
    Bacterial
    Mutation
    The fluorescent protein was inserted between XhoI…
    Available Since
    May 29, 2008
    Availability
    Academic Institutions and Nonprofits only

  20. Myc-Foxo1 AAA (pCMV5)

    Plasmid
    #17547
    Depositor
    Domenico Accili
    Insert
    Foxo1 AAA (Foxo1 Mouse)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Changed T24A T253A S316A
    Promoter
    CMV
    Available Since
    Oct. 20, 2008
    Availability
    Academic Institutions and Nonprofits only

  28. pRGE31

    Plasmid
    #50929
    Purpose
    Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by rice snoRNA U3 promoter
    Depositor
    Yinong Yang
    Article
    Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    Rice snoRNA U3 and dual 35S promoter
    Available Since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50929-plasmid-map-sequence-id-78025

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  42. pGEX-4T3-RhoA-Q63L

    Plasmid
    #12961
    Depositor
    Gary Bokoch
    Insert
    RhoA constitutively active (RHOA Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Q63L Constitutively active
    Available Since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
Showing: 8301 - 8350 of 8964 results