162,408 results

1. pcDNA5-FRT/TO-APT2-LaG16-3W

   Plasmid

   #234425

   Purpose

   Express APT2 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

   Depositor

   William Fuller

   Article

   Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

   Insert

   APT2 fused to non-binding mutant LaG16 (LYPLA2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5-FRT/TO-APT1-LaG16-3W

   Plasmid

   #234424

   Purpose

   Express APT1 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

   Depositor

   William Fuller

   Article

   Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

   Insert

   APT1 fused to non-binding mutant LaG16 (LYPLA1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AiP12493 - pAAV-AiE0374h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2493)

   Plasmid

   #230214

   Purpose

   AiE0374h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   Boaz Levi, The Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. T2A 3xNLS GFP

   Plasmid

   #227721

   Purpose

   Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

   Depositor

   Oliver Hobert, Haosheng Sun

   Article

   Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

   Insert

   T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-6P-acatenin

   Plasmid

   #186458

   Purpose

   Expresses human alpha-catenin with an N-terminal GST tag and a PRescission Protease cleavage site

   Depositor

   Tina Izard

   Article

   Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

   Insert

   CTNNA1 (CTNNA1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. DRH015_ AAV-hSyn-WT-THR

   Plasmid

   #225088

   Purpose

   Expression of wild-type thyroid receptor beta (THRB, human) with an HA tag in neurons driven by human synapsin promoter.

   Depositor

   Bernardo Sabatini

   Article

   Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

   Insert

   WT-THRB (THRB Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDRF1 mScarletI3-T2A-mTurquoise2

   Plasmid

   #219845

   Purpose

   equimolar expression of mScarletI3 and mTurquoise2 in budding yeast

   Depositor

   Bas Teusink

   Article

   Botman et al bioRxiv 2025.06.27.661975; doi: https://

   Insert

   mScarletI3-T2A-mTurquoise2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pHIV-EGFP.Flag-EZH2-mt1

   Plasmid

   #220244

   Purpose

   Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 1 (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   R27A, R31A, F165A

   Promoter

   EF-1α

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a(+)-SUMO-A'

   Plasmid

   #209115

   Purpose

   Bacterial expression of CC-GEMS ligand SUMO-A'

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   SUMO-A'
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GpACR1-pcDNA3.1-mScarlet

    Plasmid

    #204964

    Purpose

    Expression of GpACR1 in fusion with mScarlet in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

    Insert

    GpACR1
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+ enhancer)

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AiP12214 - pAAV-ProB12-SYFP2-WPRE3-BGHpA (Alias: CN2214)

    Plasmid

    #208161

    Purpose

    AAV vector for SYFP2 expression in brain astrocytes

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETM33_CLIP_CAP_Gly

    Plasmid

    #178470

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    CLIP_CAP_Gly (CLIP1 Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-HA-DEPTOR S286/287/291A

    Plasmid

    #159160

    Purpose

    Expresses DEPTOR S286/287/291A mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

    Insert

    DEPTOR (DEPTOR Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed serine 286,287 and 291 to alanine

    Promoter

    CMV

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-HA-DEPTOR S286/287/291D

    Plasmid

    #159161

    Purpose

    Expresses DEPTOR S286/287/291D mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

    Insert

    DEPTOR (DEPTOR Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed serine 286,287 and 291 to aspartic acid

    Promoter

    CMV

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p206_LTJ_sgRNACD45.2_R3

    Plasmid

    #82674

    Purpose

    sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting CD45.2 region 3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-PV-NES-GFP

    Plasmid

    #17301

    Depositor

    Anton Bennett

    Article

    Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

    Insert

    PV-NES-GFP (Pvalb Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PIA586

    Plasmid

    #104399

    Purpose

    vector for expression of single E.coli RNAP subunit

    Depositor

    Irina Artsimovitch

    Article

    Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

    Insert

    rpoD
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAID6

    Plasmid

    #136989

    Purpose

    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Inserts

    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ENO2, TDH3, TEF1, and TPI1

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-EB3mCherry_cmv-LifeActGFP

    Plasmid

    #133927

    Purpose

    Live dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeleton

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    EB3-mCherry and Lifeact-GFP (MAPRE3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-smURFP

    Plasmid

    #80349

    Purpose

    Lentiviral transfer vector under the CMV promoter that expresses smURFP.

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Insert

    smURFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSFFV_sfCherry3V(1-10)

    Plasmid

    #117483

    Purpose

    Lentiviral vector for constitutive expression of sfCherry3V(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    sfCherry3V(1-10)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    6 substitutions (K45R, G52D, L83W, T106A, K182R, …

    Promoter

    SFFV

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pVSVΔG-eGFP-linker

    Plasmid

    #231707

    Purpose

    Encodes the genome of VSV driven by a T7 polymerase promoter. It lacks the G protein (glycoprotein) and encodes eGFP.

    Depositor

    Ron Geller

    Article

    Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

    Insert

    VSV antigenome
    

    Use

    Vsv expression

    Tags

    Expression

    Mammalian

    Mutation

    ΔG (VSV glycoprotein removed)

    Promoter

    T7

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. iLID-mCl3-MAP7N

    Plasmid

    #190167

    Purpose

    Expresses iLID-mCl3-MAP7N, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    iLID-mClover3-MAP7N
    

    Use

    Tags

    iLID-mClover3

    Expression

    Mammalian

    Mutation

    aa 1-309 from human MAP7 delta175-213

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TFORF2509

    Plasmid

    #144387

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EP300 (EP300 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEB-C5

    Plasmid

    #28213

    Purpose

    5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unit

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

    Insert

    Oct4, Sox2, Klf4, c-Myc, Lin28 (Myc, Sox2, Pou5f1, Klf4, Lin28a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EGFP-Tpr

    Plasmid

    #35024

    Depositor

    Larry Gerace

    Article

    Frosst et al J Cell Biol. 2002 Feb 18;156(4):617-30.

    Insert

    Tpr (TPR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPM2213

    Plasmid

    #167553

    Purpose

    Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

    Depositor

    Renato Morona

    Article

    Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

    Insert

    rfb region of Shigella flexneri serotype 2a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 TaGu N-1F

    Plasmid

    #203762

    Purpose

    For expression of N-flag tagged TaGu R2 protein in mammalian cells

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement protein
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CMV-R-GECO1

    Plasmid

    #32444

    Purpose

    Mammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    R-GECO1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Ddo_piwi-1

    Plasmid

    #107628

    Purpose

    In situ hybridization and RNAi studies in planarians

    Depositor

    Alejandro Sánchez

    Article

    Accorsi et al The American Biology Teacher 79(3):208-2

    Insert

    piwi-1
    

    Use

    RNAi; In situ hybridization

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBFC0984

    Plasmid

    #231992

    Purpose

    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site

    Depositor

    Brady Cress

    Article

    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

    Inserts

    dRfxCas13d
    crRNA with 2xBsaI spacer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 and pTet

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. qTAG-C-dTAG-Zeo

    Plasmid

    #207738

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pFUW-TetO-Fos

    Plasmid

    #131593

    Purpose

    doxycycline-inducible expression of mouse c-Fos in mammalian cells

    Depositor

    Kateri Moore, Filipe Pereira

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    FBJ osteosarcoma oncogene (Fos Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CSTB_pCSdest

    Plasmid

    #53910

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    CSTB (CSTB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    Sept. 8, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

35. pCAG-mNaChBac-T2A-tdTomato

    Plasmid

    #60650

    Purpose

    Expresses mNaChBac-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    mNaChBac-T2A-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-MCN-10His-mStayGold (E138D)

    Plasmid

    #211361

    Purpose

    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Bacterial

    Mutation

    E138D

    Promoter

    T7

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MSCV Luciferase PGK-hygro

    Plasmid

    #18782

    Depositor

    Scott Lowe

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHLUM

    Plasmid

    #40276

    Depositor

    Markus Ralser

    Article

    Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

    Inserts

    LEU2 (LEU2 Budding Yeast)
    URA3 (URA3 Budding Yeast)
    MET15/17 (MET17 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    endogenous LEU2, endogenous MET15/17, and endogen…

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDmDERusk

    Plasmid

    #213702

    Purpose

    Vector for red-light-induced bacterial gene expression

    Depositor

    Andreas Moeglich

    Article

    Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. rAAV2/cMG

    Plasmid

    #184539

    Purpose

    AAV packaging plasmid, expressing Rep2 and AAV-cMG capsid

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

    Insert

    AAV-cMG capsid
    

    Use

    AAV; Helper vector for packaging

    Tags

    Expression

    Mutation

    changes on AAV9 capsid: K449R, A589D; insertion o…

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMSCV-NLS-CBRed-d2-hygro

    Plasmid

    #139196

    Purpose

    non-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puro

    Depositor

    Matthew Lazzara

    Article

    Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

    Insert

    CBRed
    

    Use

    Luciferase and Retroviral

    Tags

    d2 PEST domain and nuclear localization signal (N…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Talin-TS

    Plasmid

    #83376

    Purpose

    FRET probe for measuring tension on Talin molecule

    Depositor

    Martin Schwartz

    Article

    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

    Insert

    Talin1 (Tln1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-F40-tagRFP (tension sensor module)

    Expression

    Mammalian

    Mutation

    Tension sensor module is inserted between V447 an…

    Promoter

    CMV

    Available since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAAV_GR-RE_MLPmin_BC1092-luc2

    Plasmid

    #227112

    Purpose

    Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1092

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered GR response element linked to MLP minimal promoter driving barcode 1092 and a luciferase reporter gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. SP6-VEE-IRES-Puro

    Plasmid

    #58971

    Purpose

    noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Type

    Empty backbone

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    26S subgenomic promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCMV-Sport6-CD63-pHuji

    Plasmid

    #130902

    Purpose

    Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHuji (CD63 Human, synthetic)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Gln-36 and Leu-37 in extra…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pHAGE-BRAF-V600E

    Plasmid

    #116204

    Purpose

    Lentiviral expression of BRAF V600E

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    V600E

    Promoter

    Available since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. CXCR2-DuET

    Plasmid

    #213219

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR2 (CXCR2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCAG_smFP HA

    Plasmid

    #59759

    Purpose

    encodes multiple copies of an epitope tag HA

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _HA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA

    Plasmid

    #183776

    Purpose

    AAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

    Depositor

    Ben Deverman

    Article

    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

    Insert

    Cre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    CAG

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pDual-eGFP

    Plasmid

    #63215

    Purpose

    Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV-EF1α hybrid (CEF)

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only