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19,770 results

Showing: 9051 - 9100 of 19770 results

  7. NanoLuc-PM

    Plasmid
    #164784
    Purpose
    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors
    Depositor
    Laszlo Hunyady
    Article
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Insert
    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Oplophorus gracilirostris, Human)
    Tags
    NanoLuc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164784-plasmid-map-sequence-id-326673

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  10. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69545
    Purpose
    Tet-On system expressing GFP tagged with 2x nuclear localization signals.
    Depositor
    Charles Gersbach
    Insert
    eGFP
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69545-plasmid-map-sequence-id-125605

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  20. AICSDP-47: CTNNB1-mEGFP

    Plasmid
    #109119
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human CTNNB1
    Depositor
    Allen Institute for Cell Science
    Insert
    CTNNB1 Homology Arms with mEGFP-linker (CTNNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    May 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    109119-plasmid-map-sequence-id-209765

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  25. pcDNA3-HA-SOX4

    Plasmid
    #110367
    Purpose
    Mammalian expression of HA-tagged SOX4
    Depositor
    Carlos Moreno
    Insert
    SOX4 (SOX4 Human)
    Tags
    HA
    Expression
    Mammalian
    Available Since
    Jan. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    110367-plasmid-map-sequence-id-211161

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  31. pET-Trx-mSA2

    Plasmid
    #52320
    Purpose
    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
    Depositor
    Sheldon Park
    Article
    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6
    Insert
    monomeric streptavidin 2
    Tags
    FLAG, His, S tag, TEV site, and Thioredoxin
    Expression
    Bacterial
    Mutation
    S25H mutation compared to streptavidin
    Promoter
    T7
    Available Since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52320-plasmid-map-sequence-id-84861

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  33. pCMV6-AC EJ7-GFP

    Plasmid
    #113617
    Purpose
    Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP
    Depositor
    Jeremy Stark
    Article
    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:
    Insert
    EJ7-GFP variant of EGFP
    Expression
    Mammalian
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    113617-plasmid-map-sequence-id-218254

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  35. sfGFP-Peroxisomes-2

    Plasmid
    #54601
    Purpose
    Localization: Peroxisomes, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Insert
    sfGFP-Peroxisomes
    Tags
    Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54601-plasmid-map-sequence-id-267741

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  45. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

    Plasmid
    #140005
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140005-plasmid-map-sequence-id-275488

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Showing: 9051 - 9100 of 19770 results