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159,141 results

Showing: 7401 - 7440 of 159141 results

  1. pLV-Enh eGFP Reporter-muKC2-X1

    Plasmid
    #59200
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    krt1-ex1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. pcDNA3-TLR6-YFP

    Plasmid
    #13020
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 6 (TLR6 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13020-plasmid-map-sequence-id-7420

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  3. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-99784

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  5. p7SL-neo-TET

    Plasmid
    #51285
    Purpose
    expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Article
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    7SL RNA (RN7SL2 Human)
    Use
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Mutation
    Promoter
    7SL upstream pollII enhancer
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51285-plasmid-map-sequence-id-78367

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  7. pLenti-EGFP-SnxA

    Plasmid
    #232460
    Purpose
    Lentiviral EGFP lipid sensor for PI(3,5)P2
    Depositor
    Ken-Ichi Takemaru
    Insert
    EGFP-SnxA
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    March 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    232460-plasmid-map-sequence-id-464306

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  12. CUP1-∆ssCPY*-mGFP in pRS315

    Plasmid
    #221158
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mGFP
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    221158-plasmid-map-sequence-id-450962

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  13. CUP1-∆ssCPY*-mScarletI in pRS315

    Plasmid
    #221159
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mScarletI
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221159-plasmid-map-sequence-id-450960

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  16. pQE81XN-msYFP

    Plasmid
    #218960
    Purpose
    Expresses Hisx6-Tagged msYFP in bacteria
    Depositor
    Benjamin Glick
    Insert
    msYFP
    Use
    Tags
    6X-His
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218960-plasmid-map-sequence-id-446223

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  17. PRS_8-GCaMP8m

    Plasmid
    #210400
    Purpose
    AAV-vector for fluorescent calcium measurements in the green spectrum from noradrenergic neurons
    Depositor
    Simon Wiegert
    Article
    Wissing et al bioRxiv 2022.01.22.477348
    Insert
    GCaMP8m
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRSx8
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    210400-plasmid-map-sequence-id-415628

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  20. CXCR1-DuET

    Plasmid
    #213218
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CXCR1 (CXCR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213218-plasmid-map-sequence-id-430780

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  21. CCR6-DuET

    Plasmid
    #213202
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR6 (CCR6 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213202-plasmid-map-sequence-id-430765

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  22. CCR3-DuET

    Plasmid
    #213199
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR3 (CCR3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213199-plasmid-map-sequence-id-430762

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  23. CCR4-DuET

    Plasmid
    #213200
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR4 (CCR4 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213200-plasmid-map-sequence-id-430763

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  29. pdCas9

    Plasmid
    #46569
    Purpose
    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9
    CRISPR array
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46569-plasmid-map-sequence-id-364492

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  32. pET30b_OmpAT7Sav

    Plasmid
    #138589
    Purpose
    T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori
    Depositor
    Markus Jeschek
    Article
    Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d
    Insert
    T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
    Use
    Synthetic Biology
    Tags
    OmpA export signal and T7 tag
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    138589-plasmid-map-sequence-id-274488

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  37. pCMV-2E2-HA-mEGFP

    Plasmid
    #129595
    Purpose
    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129595-plasmid-map-sequence-id-254267

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Showing: 7401 - 7440 of 159141 results