160,950 results

1. Brm WT-Flag pShuttle

   Plasmid

   #122105

   Purpose

   Expression of full length Brm in mammalian cells

   Depositor

   Paul Herring

   Article

   Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

   Insert

   BRM (SMARCA2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA

   Plasmid

   #196192

   Purpose

   human synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
   

   Use

   Znf donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAd5-B5

   Plasmid

   #122555

   Purpose

   Block 5 for AdenoBuilder genome assembly

   Depositor

   Fred Bunz

   Article

   Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

   Insert

   Adenovirus 5 genomic region 17998-25062
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. miniCGBE1 (pBM1219)

   Plasmid

   #140253

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   BE4max(R33A)-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in Cas9

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pnCS_SEPT7_SEPT9_i1-TEV-Strep

   Plasmid

   #174500

   Purpose

   bacterial co-expression of human SEPT7 and of human SEPT9_i1

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN7 (SEPTIN7 Human)
   SEPTIN9 (SEPTIN9 Human)
   

   Use

   Tags

   TEV-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOpen-T4 g32

   Plasmid

   #165560

   Purpose

   A single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   T4 gene 32
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pBB572

   Plasmid

   #27397

   Depositor

   Bernd Bukau

   Article

   de Marco et al BMC Biotechnol. 2007 . 7():32.

   Insert

   ibpA, ibpB (ibpB, ibpA E.coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCM184-ParB2::eGFP

   Plasmid

   #87252

   Purpose

   Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   ParB2
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   pBAD/tetO

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCJ136

   Plasmid

   #162665

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. METTL3 shRNA 2 tet pLKO puro

    Plasmid

    #162984

    Purpose

    Tet-inducible shRNA targeting human METTL3 #2

    Depositor

    Mark Rubin

    Article

    Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET42b-BE3

    Plasmid

    #87437

    Purpose

    Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

    Insert

    BE3 (Apobec1 Bacillus phage PBS2, Human, Rat)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223-HK2

    Plasmid

    #23854

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    HK2 (HK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. PB-CMV-MCS-Puro-EGFP

    Plasmid

    #219795

    Purpose

    Mammalian expression plasmids using piggyBac Transposon System

    Depositor

    Keiichi Nakayama

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJG100

    Plasmid

    #138966

    Purpose

    Peft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegans

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    wrmScarlet1-10
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-syn-flex-iGluSnFR4f-NGR-WPRE

    Plasmid

    #234442

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-NGR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti.PGK.LifeAct-Ruby.W

    Plasmid

    #51009

    Purpose

    Lentivector that expresses LifeAct-Ruby from internal human PGK promoter

    Depositor

    Rusty Lansford

    Insert

    LifeAct-mRFPruby
    

    Use

    Lentiviral

    Tags

    LifeAct

    Expression

    Mammalian

    Mutation

    Promoter

    human PGK

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-U6-sgRNA-PGK-puromycin

    Plasmid

    #51133

    Purpose

    expresses sgRNA from the U6 promoter.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-LMNA-gRNA1

    Plasmid

    #122507

    Purpose

    expresses WT spCas9 and a chimeric gRNA targeting human LMNA

    Depositor

    Graham Dellaire

    Article

    Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

    Insert

    LMNA-gRNA1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-p38alpha

    Plasmid

    #86832

    Purpose

    mammalian expression of GFP-p38alpha

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    p38alpha (MAPK14 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-FGFR3

    Plasmid

    #116742

    Purpose

    Lentiviral expression of FGFR3

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FGFR3 (FGFR3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro

    Plasmid

    #141374

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp8 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pWM_12x601_15bpLinker

    Plasmid

    #157786

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_15bp linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCI-EGFP-NR2b wt

    Plasmid

    #45447

    Depositor

    Robert Malinow, Andres Barria

    Article

    Barria et al Neuron. 2002 Jul 18;35(2):345-53.

    Insert

    NR2B (Grin2b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EGFP inserted after the predicted signal peptide …

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAV-Syn-PSD95-mCherry

    Plasmid

    #125694

    Purpose

    Exogenous expression of PSD95 (red fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    PSD95-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJG103

    Plasmid

    #158533

    Purpose

    wrmScarlet11 x3 tandem repeats

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    wrmScarlet11 x3 tandem repeats
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pOpen-KTHEAQpol

    Plasmid

    #165542

    Purpose

    Exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    KlenTaq1 polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pcDNA5-Flag-BRD4-WT

    Plasmid

    #90331

    Purpose

    expresses Wild type BRD4

    Depositor

    Kornelia Polyak

    Article

    Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PER2:Luc Plasmid

    Plasmid

    #206875

    Purpose

    Per2 promoter luciferase reporter

    Depositor

    Michelle Farkas

    Article

    Lin et al Integr Cancer Ther. 2019 Jan-Dec;18:1534

    Insert

    Period 2 (Per2 Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFastbac1-Flag-FANCD2

    Plasmid

    #134904

    Purpose

    codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Insert

    FANCD2 (FANCD2 Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    fully synthetic, codon optimized for insect expre…

    Promoter

    polyhedron

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDNA3 Flag p38 alpha

    Plasmid

    #20351

    Depositor

    Roger Davis

    Article

    Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

    Insert

    pDCNA3 Flag p38 alpha (Mapk14 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge