165,144 results

1. pLenti CMV Neo DEST (705-1)

   Plasmid

   #17392

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. dg265_pENTR_CoChRCel

   Plasmid

   #66103

   Purpose

   Entry clone containing CoChR coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

   Depositor

   Dominique Glauser

   Article

   Schild et al Genetics. 2015 May 28. pii: genetics.115

   Insert

   CoChR
   

   Use

   Gateway

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKRaION1-N1

   Plasmid

   #177801

   Purpose

   Mammalian expression of green fluorescent potassium indicator KRaION1

   Depositor

   Edward Boyden

   Article

   Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

   Insert

   KRaION1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-CMV-hIL7-IRES-ZsGreen-W

   Plasmid

   #26532

   Depositor

   David Baltimore

   Insert

   IL7 (IL7 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC13N-dCas9-BFP-KRAB

   Plasmid

   #127968

   Purpose

   constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   CLYBL-CAG-dCas9-NLS-BFP-KRAB
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Synapto-pHluorin

   Plasmid

   #168510

   Purpose

   Expression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   Synaptophysin-pHluorin (Syp Mouse)
   

   Tags

   pHluorin

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

   Plasmid

   #219565

   Purpose

   3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome

   Depositor

   Andrea Ventura

   Article

   Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

   Insert

   HygroR_2A_H2B-GFP-N_loxP_mScarlet
   

   Use

   Unspecified

   Promoter

   EF-1a

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. phL1A-pcDNA3

   Plasmid

   #12307

   Depositor

   Vance Lemmon

   Article

   Hlavin et al Genomics. 1991 Oct . 11(2):416-23.

   Insert

   L1CAM (L1CAM Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Myc-NLRP3(R260W)

   Plasmid

   #73956

   Purpose

   Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

   Depositor

   Christian Stehlik

   Article

   Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

   Insert

   NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
   

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   R260W

   Promoter

   CMV iE

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCZGY3163

    Plasmid

    #101248

    Purpose

    GFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.

    Depositor

    Yishi Jin

    Article

    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

    Insert

    gfp11::rps-18 (rps-18 Nematode, Synthetic)
    

    Use

    Gateway

    Tags

    GFP11

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1-MAFB-IRF8-CEBPA

    Plasmid

    #190720

    Purpose

    For microglia differentiation from iPSC

    Depositor

    Li Gan

    Article

    Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

    Insert

    MAFB-IRF8-CEBPA
    

    Expression

    Mammalian

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTriEx-mCherry-LOV2

    Plasmid

    #81041

    Purpose

    Mammalian or insect expression for wt LOV2

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV2
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV, p10

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PKC zeta DN

    Plasmid

    #21249

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC zeta (Prkcz Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K281M

    Available Since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

14. FSW_jGCaMP8f

    Plasmid

    #197034

    Purpose

    Express GCaMP8f from hSyn1 promoter

    Depositor

    Ronald Hart

    Insert

    GCaMP8f
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+i)

    Plasmid

    #192123

    Purpose

    Lentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(d+i)

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_41BB_NS3(s139a)_CD3z-mCherry
    

    Use

    Lentiviral

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pY016 (pcDNA3.1-hLbCpf1)

    Plasmid

    #69988

    Purpose

    Expresses humanized LbCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. PKC eta DN

    Plasmid

    #21246

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC eta (Prkch Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K384R

    Available Since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pHW449

    Plasmid

    #198805

    Purpose

    Direct light-gated cation channel from Chlamydomonas reinhardtii that allows neuron deploarization through brief pulses of blue light.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::hChR2(H134R)-YFP::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. dSaCas9-KRAB

    Plasmid

    #205250

    Purpose

    In vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.

    Depositor

    Rasmus Bak

    Insert

    dSaCas9-KRAB
    

    Use

    CRISPR

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMLS328

    Plasmid

    #73717

    Purpose

    C. elegans germline CRE expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    2xNLS-CRE
    

    Use

    Cre/Lox

    Expression

    Worm

    Promoter

    Peft-3

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pHJL2

    Plasmid

    #199377

    Purpose

    Encodes dual cistronic cytosolic GCaMP6f and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    GCaMP6f::SL2::mScarlet-I
    

    Expression

    Bacterial

    Mutation

    no mutations

    Available Since

    April 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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22. pCMVtight-RFP-IRES-CD

    Plasmid

    #126678

    Purpose

    Doxycycline inducible expression of CD along with reporter RFP

    Depositor

    Joan Massague

    Article

    Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

    Insert

    RFP-IRES-CD (FCY1 Budding Yeast)
    

    Promoter

    tight TRE

    Available Since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid

    #84257

    Purpose

    Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9-ABI
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pSH83

    Plasmid

    #212989

    Purpose

    expresses miniSOG in C. elegans

    Depositor

    David M. Miller

    Article

    Cuentas-Condori et al Elife. 2019 Oct 4;8. pii: 47918. doi: 10

    Insert

    flp-13 promoter (flp-13 Nematode)
    

    Expression

    Worm

    Promoter

    flp-13

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. SPLICS Po-Mt Long P2A

    Plasmid

    #164115

    Purpose

    Detect the long Peroxisomes-mitochondria contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Po-Mt Long P2A
    

    Expression

    Mammalian

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pDONR223-ADRBK2

    Plasmid

    #23793

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ADRBK2 (AA1-387) (GRK3 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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27. GFP-JIP3

    Plasmid

    #164624

    Purpose

    Expresses JIP3 with a GFP tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    JIP3 (MAPK8IP3 Human)
    

    Tags

    cycle 3 GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCBEclos-cbei2385-g2-opt

    Plasmid

    #118215

    Purpose

    CRISPR-mediated base editing in Clostridium

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol Bioeng. 2019 Feb 10. doi: 10.

    Insert

    Apobec1, Casp nickase, UG1-sgRNA
    

    Expression

    Bacterial

    Promoter

    Ptm

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. pAS3-lim-4P_gtACR2_SL2_eGFP(65C)_unc-54 3'UTR

    Plasmid

    #195853

    Purpose

    Expresses inhibitory opsin gtACR2 and GFP in turning associated neurons

    Depositor

    Andrew Leifer

    Article

    Kumar et al PLoS Biol. 2023 Sep 21;21(9):e3002280. d

    Insert

    Guillardia theta anion-conducting channelrhodopsin-2 (GtACR2)
    

    Expression

    Worm

    Promoter

    lim-4

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCMVHA EED wt

    Plasmid

    #24231

    Purpose

    Mammalian expression of human EED with HA tag

    Depositor

    Kristian Helin

    Article

    Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

    Insert

    EED (EED Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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