162,275 results

1. pSynSRE-T-Luc

   Plasmid

   #60444

   Purpose

   Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

   Depositor

   Timothy Osborne

   Article

   Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

   Insert

   HMG-CoA synthase promoter (-324/-225)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

   Available since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.CAG.Flex.GCaMP6f.WPRE.SV40

   Plasmid

   #100835

   Purpose

   AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

   Promoter

   CAG

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCnRed

   Plasmid

   #232908

   Purpose

   Electroporation vector for expression of the lambda Red recombineering system in Cupriavidus necator.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

   Insert

   gam (gam phage Lambda, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Pbad-rha

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

   Plasmid

   #92392

   Purpose

   produces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
   

   Use

   AAV and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human Synapsin

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRFHUE-eGFP

   Plasmid

   #89469

   Purpose

   GFP labelling of filamentous fungi by Agrobacterium tumefaciens mediated transformation

   Depositor

   Luis González-Candelas

   Article

   Crespo-Sempere et al Int J Food Microbiol. 2011 Aug 2;148(2):

   Insert

   eGFP
   

   Use

   Binary plasmid for atmt of filamentous fungi

   Tags

   Expression

   Mutation

   Promoter

   A. nidulans gpdA

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSBbi-pur H-2Kb

   Plasmid

   #111623

   Purpose

   Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele

   Depositor

   Jon Yewdell

   Insert

   H2Kb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-DIO-mCherry

   Plasmid

   #50459

   Purpose

   Double floxed mCherry under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   mCherry
   

   Use

   AAV

   Tags

   N/A

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFD5

   Plasmid

   #73914

   Purpose

   multiplex gRNA plasmid

   Depositor

   Simon Bullock

   Article

   Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

   Insert

   dU6-3:tRNA:gRNA:tRNA:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6:3

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSCAR_Cas9-blast_GFP

   Plasmid

   #162074

   Purpose

   3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Inserts

   Cas9-2a-blasticidin S deaminase
   EGFP
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in M. musculus

   Promoter

   EF1a and sv40

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. IRE1 alpha-pcDNA3.EGFP

    Plasmid

    #13009

    Depositor

    Fumihiko Urano

    Article

    Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

    Insert

    Inositol Requiring Enzyme-1 (ERN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDCC6

    Plasmid

    #59985

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Inserts

    Cas9
    U6-2>sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    U6-96Ab and hsp70Bb

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST40-2XFL-Src-K298M

    Plasmid

    #140315

    Purpose

    Expresses Flag-tagged human Src-K298M in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    K298M

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCE-KdGl

    Plasmid

    #154880

    Purpose

    Episomally expresses KDM4D and GLIS1 in mammalian cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

    Insert

    KDM4D, GLIS1 (KDM4D, GLIS1 Human, Callithrix jacchus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLJC2-Blast-SHMT2-3xFLAG

    Plasmid

    #233891

    Purpose

    lentiviral overexpression vector for SHMT2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

    Insert

    SHMT2 (SHMT2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. TagBFP_A1aY1

    Plasmid

    #158752

    Purpose

    Lentiviral vector for expression of TagBFP_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

    Insert

    TagBFP-T_A1aY1
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG (CMV enhancer and chicken beta actin promoter)

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD-mhYFP

    Plasmid

    #186532

    Purpose

    Monomeric hyperfolder YFP fluorescent protein: bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Monomeric hyperfolder YFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    hfYFP-S147P/L195M/V206K

    Promoter

    araB

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. spMSP1D1

    Plasmid

    #173482

    Purpose

    Expresses spMSP1D1 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spMSP1D1
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-VTKS2-TVA-eGFP-N2cG

    Plasmid

    #175439

    Purpose

    Cre-on (DIO) helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

    Depositor

    Gordon Fishell

    Article

    Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

    Insert

    TVA-P2A-eGFP-P2A-N2cG
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLOX-CW-CRE

    Plasmid

    #12238

    Depositor

    Didier Trono

    Article

    Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

    Insert

    CRE
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    CMV promoter is upstream of nls-CRE. LoxP in 3&#x…

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQCascade

    Plasmid

    #140622

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNA

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HRE-dUnOHR

    Plasmid

    #201714

    Purpose

    Hypoxia responsive promoter driving UnaG and mOrange

    Depositor

    Friedemann Kiefer

    Article

    Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

    Insert

    UnaG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TFORF2509

    Plasmid

    #144387

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EP300 (EP300 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CMV mRuby3-Synapsin1a

    Plasmid

    #187896

    Purpose

    For labeling pre-synaptic terminals

    Depositor

    Timothy Ryan

    Insert

    Synapsin1a
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRK5-FLAG-p14

    Plasmid

    #42330

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

    Insert

    p14 (LAMTOR2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pREDawn-StrR-MCS

    Plasmid

    #188979

    Purpose

    For red light-induced bacterial expression of gene of interest (StrR)

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHAGE-DDX3X

    Plasmid

    #116730

    Purpose

    Lentiviral expression of DDX3X

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    DDX3X (DDX3X Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-Hs.cargo(Cre)

    Plasmid

    #174863

    Purpose

    Expresses human Peg10 cargoRNA encoding Cre recombinase

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    Cre flanked by HsPeg10 UTRs
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pF151 pcDNA3.1(+)SOD1WT

    Plasmid

    #26397

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAV CAMKII PSAM4 GlyR IRES EGFP

    Plasmid

    #119744

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    CamKII

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAV-CMV-Nrf2

    Plasmid

    #67636

    Purpose

    AAV vector expressing Nrf2 gene

    Depositor

    Connie Cepko

    Article

    Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

    Insert

    Nrf2 (Nfe2l2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pICH41421

    Plasmid

    #50339

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV-EB3mCherry_cmv-LifeActGFP

    Plasmid

    #133927

    Purpose

    Live dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeleton

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    EB3-mCherry and Lifeact-GFP (MAPRE3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPM2213

    Plasmid

    #167553

    Purpose

    Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

    Depositor

    Renato Morona

    Article

    Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

    Insert

    rfb region of Shigella flexneri serotype 2a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. 1481 pcDNA3 flag HA 14-3-3 zeta

    Plasmid

    #9002

    Depositor

    William Sellers

    Insert

    14-3-3 zeta (YWHAZ Bovine)
    

    Use

    Tags

    HA and flag

    Expression

    Mammalian

    Mutation

    T226A

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEvol-pAzFRS.1.t1

    Plasmid

    #73547

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAzFRS.1.t1
    pAzFRS.1.t1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRwB

    Plasmid

    #48734

    Purpose

    dsRed plasmid

    Depositor

    John Schiller

    Article

    Johnson et al J Virol. 2009 Mar;83(5):2067-74. Epub 20

    Insert

    dsRed-Express
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDH-EF1-IRES eGFP

    Plasmid

    #128059

    Purpose

    Lentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-CaMKIIa-ChRmine-mScarlet-WPRE

    Plasmid

    #130990

    Purpose

    AAV vector to drive the expression of ChRmine-mScarlet under the control of CamKIIa promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p3258 pCMV hUBC9 wt HA

    Plasmid

    #14438

    Depositor

    Peter Howley

    Article

    Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

    Insert

    UBC9 (UBE2I Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CSTB_pCSdest

    Plasmid

    #53910

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    CSTB (CSTB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    Sept. 8, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits