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175,647 results

Showing: 10001 - 10050 of 175647 results

  1. AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60225
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    Oct. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    60225-plasmid-map-sequence-id-96391

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  4. CMVp-dsRed2-Triplex-28-gRNA1-28-pA

    Plasmid
    #55200
    Purpose
    Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Article
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Insert
    dsRed2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    55200-plasmid-map-sequence-id-85587

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  5. mEmerald-mDia1-N-14

    Plasmid
    #54157
    Purpose
    Localization: Cytoskeleton, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    mDia1 (DIAPH1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54157-plasmid-map-sequence-id-475534

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  6. mEmerald-Ezrin-N-14

    Plasmid
    #54090
    Purpose
    Localization: Focal Adhesions/Membrane, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Ezrin (EZR Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54090-plasmid-map-sequence-id-243987

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  7. PA-mCherry1-C1

    Plasmid
    #54495
    Purpose
    Localization: C1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    PA-mCherry1
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    54495-plasmid-map-sequence-id-218682

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  8. PA-mCherry1-N1

    Plasmid
    #54507
    Purpose
    Localization: N1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    PA-mCherry1
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. mCit-4aa-SNAP25B

    Plasmid
    #53235
    Purpose
    Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    SNAP-25B (Snap25 Rat)
    Tags
    4aa-linker and mCitrine
    Expression
    Mammalian
    Available Since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    53235-plasmid-map-sequence-id-421058

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  10. mCer-4aa-VAMP2

    Plasmid
    #53233
    Purpose
    Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    VAMP2 (Vamp2 Rat)
    Tags
    4aa-linker and mCerulean
    Expression
    Mammalian
    Available Since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50467
    Purpose
    Gi-coupled hM4D-IRES-mCitrine under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has Service
    AAV2 and AAV8
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50467-plasmid-map-sequence-id-199198

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  15. pCAG-Co-InCreN

    Plasmid
    #51267
    Purpose
    The N-terminal component of the Co-InCre system, expresses the N-Cre-N-gp41-1 fusion that generates functional Cre by protein trans-splicing upon reacting with Co-InCreC
    Depositor
    Pawel Pelczar
    Article
    Hermann et al Nucleic Acids Res. 2014 Jan 9.
    Insert
    Co-InCreN
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51267-plasmid-map-sequence-id-77878

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  16. pCAG-Co-InCreC

    Plasmid
    #51268
    Purpose
    The C-terminal component of the Co-InCre system, expresses the C-gp41-1-C-Cre fusion that generates functional Cre by protein trans-splicing upon reacting with Co-InCreN
    Depositor
    Pawel Pelczar
    Article
    Hermann et al Nucleic Acids Res. 2014 Jan 9.
    Insert
    Co-InCreC
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51268-plasmid-map-sequence-id-77879

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  17. pN565

    Plasmid
    #49990
    Purpose
    expresses an attenuated T7 RNAP under an isopropyl β-d-1-thiogalactopyranoside (IPTG) inducible control
    Depositor
    Christopher Voigt
    Article
    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1
    Insert
    T7 RNAP*
    Use
    Synthetic Biology
    Tags
    umuD degradation tag
    Expression
    Bacterial
    Mutation
    R632S
    Promoter
    pTac-symmetric
    Available Since
    Jan. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    49990-plasmid-map-sequence-id-384231

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  18. pGL3 basic E2

    Plasmid
    #48747
    Purpose
    Expression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    mPer2 promoter/enhancer region containing E-box2 (-112 to +98)
    Use
    Luciferase
    Available Since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    48747-plasmid-map-sequence-id-444692

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  23. CMV-mito-CAR-GECO1

    Plasmid
    #46022
    Purpose
    Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging
    Depositor
    Robert Campbell
    Article
    Wu et al ACS Chem Neurosci. 2013 Mar 19.
    Insert
    CAR-GECO1
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P
    Promoter
    CMV
    Available Since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  48. pET283xFlagPP7CPHis

    Plasmid
    #28174
    Depositor
    Kathleen Collins
    Insert
    PP7 coat protein optimized
    Tags
    6xhistidine and triple FLAG peptide
    Expression
    Bacterial
    Mutation
    C68A,C73A
    Available Since
    Feb. 24, 2011
    Availability
    Academic Institutions and Nonprofits only
    28174-plasmid-map-sequence-id-13350

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  49. pLenti CMVtight eGFP Hygro (w783-1)

    Plasmid
    #26585
    Purpose
    2nd generation lentiviral vector
    Depositor
    Eric Campeau
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Lentiviral; Tet-on advanced vector
    Expression
    Mammalian
    Available Since
    Jan. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    26585-plasmid-map-sequence-id-499878

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Showing: 10001 - 10050 of 175647 results