173,271 results
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pRc/CMV HA TrkA
Plasmid#39977DepositorInsertTrkA (Ntrk1 Rat)
TagsBM40 signal sequence and HAExpressionMammalianMutationendogenous signal sequence was replaced with BM40…PromoterCMVAvailable SinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKD3_mCherry
Plasmid#187385PurposeTo chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRTDepositorInsertmCherry
ExpressionBacterialAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-TOMM20-N-10
Plasmid#123284PurposeLocalization: Outer Mitochondrial MembraneDepositorAvailable SinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9-VP64
Plasmid#50916PurposeTet-regulatable dCas9-VP64 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and VP64ExpressionMammalianMutationD10A, H840A nuclease-deficientPromoterTREAvailable SinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mRuby3-10aa-H2B
Plasmid#74258PurposeMammalian expression of N-terminal fusion of mRuby3 to H2B.DepositorInsertH2B
TagsmRuby3ExpressionMammalianPromoterCAG and CMVAvailable SinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_WWAV_GPC
Plasmid#207314PurposeMammalian expression of Whitewater Arroyo virus GPC proteinDepositorInsertWWAV_GPC
ExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (WT)
Plasmid#169720PurposeMammalian Expression of wild-type mouse SPOPDepositorAvailable SinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nv5-ER beta
Plasmid#22770DepositorAvailable SinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
AiP20142 - pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA
Plasmid#230803PurposeAiE2638m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsDepositorInsertiCre(R297T)
UseAAVPromoterminBGAvailable SinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-MITF-A
Plasmid#38132DepositorAvailable SinceJuly 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQE81XN-mStayGold(J)
Plasmid#225445PurposeExpresses Hisx6-Tagged mStayGold(J) in bacteriaDepositorInsertmStayGold(J)
Tags6X-HisExpressionBacterialAvailable SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EGFR-L858R
Plasmid#116276PurposeLentiviral expression of EGFR L858RDepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-COX4-dAPEX2
Plasmid#117176PurposePlasmid name in publication: pAAV-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertCOX4-dAPEX2
UseAAVExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
dCas13d-dsRBD-APEX2
Plasmid#154939PurposeTetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFPDepositorInsertTetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP
TagsV5ExpressionMammalianPromoterTRE3GAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIR3DL3.1-Fc(DAPA)-AviTag-6xHis
Plasmid#156735PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertKIR3DL3 (KIR3DL3 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-OSBP-PH
Plasmid#49571Purposeexpresses the plekstrin homology domain of OSBP in mammalian cellsDepositorAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC8 human IFN-gamma
Plasmid#17600DepositorInsertinterferon-gamma cDNA (IFNG Human)
ExpressionBacterialAvailable SinceMay 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMC051-HisMS2_PLP_pac_LC
Plasmid#128234PurposeLower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.DepositorInsertsMaturation Protein
Coat Protein Dimer
ExpressionBacterialPromoterT7Available SinceNov. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pScaf-7560.1
Plasmid#111406PurposepScaf phagemid with insert for generating DNA origami scaffold 7560 bases in lengthDepositorInsertpScaf-7560.1
ExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
MaLionR
Plasmid#113908PurposeRed ATP sensorDepositorInsertMaLionR
ExpressionMammalianAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Nkx2-1/Puro
Plasmid#31271DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-MRE11-WT
Plasmid#82033PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol2-DRv7
Plasmid#78627PurposeGESTALT V7 barcode for zebrafishDepositorInsertGESTALT lineage tracing target V7
Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5_mCherry_P2AT2A_GFP-nb
Plasmid#238232PurposeFor overexpression of mCherry_P2AT2A_GFP-nbDepositorInsertmCherry_P2AT2A_GFP-nb
ExpressionMammalianAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA
Plasmid#108871PurposeExpression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafishDepositorInserts5xU6:sgRNA
Cas9-t2A-GFP
UseCRISPRPromoterU6 and hsp70Available SinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mNeptune2.5
Plasmid#51310PurposeExpresses far-red fluorescent protein mNeptune2.5 in mammalian cellsDepositorInsertmNeptune2.5
ExpressionMammalianPromoterCMVAvailable SinceApril 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-SB-NLS
Plasmid#228870PurposePE7 connected to MLH1-SB-NLS via 2A sequenceDepositorInsertPE7-2A-MLH1-SB-NLS
UseCRISPRAvailable SinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVP074
Plasmid#222077PurposeeforRED visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.DepositorTypeEmpty backboneUseChromoprotein-marked pvs1 backboneAvailable SinceJan. 27, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDest-mCherry-N1
Plasmid#31907DepositorHas ServiceCloning Grade DNATypeEmpty backboneExpressionMammalianPromoterCMVAvailable SinceNov. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/CD63mScarlet
Plasmid#182972PurposeStably express CD63 fused with mScarlet in mammalian cells via the sleeping beauty transposon system.DepositorAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM-shRnd3
Plasmid#86437Purpose2nd generation doxycycline-inducible lentiviral vector expressing Rnd3-targeting shRNA and GFPDepositorAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-BFP
Plasmid#78545PurposeFusion protein Cas9+BFPDepositorInsertBFP
UseLentiviralExpressionMammalianPromoterFusion protein with Cas9Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTubb3-MC
Plasmid#87112Purposeminicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITIDepositorInsertGFP
UseCRISPRAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 (-) + codon optimized Brn3a
Plasmid#86829Purposemammalian expression of the transcription factor Brn3aDepositorAvailable SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AsCas12f1
Plasmid#171614PurposeAsCas12f1-based Human cell genome editingDepositorInsertAsCas12f1 and sgRNA_v1
ExpressionMammalianAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis).iGluSnFR
Plasmid#41732PurposeSingle-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertGltI based Glutamate Biosensor
UseGlutamate biosensorTagsMyc, PDGFR transmembrane helix (aa 513-561), and …ExpressionMammalianMutationcpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…PromoterCMVAvailable SinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)
Plasmid#217808PurposeExpression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)DepositorInsert5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterT7Available SinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET303-colE1TR
Plasmid#155181PurposeExpresses colicin E1 residues 1-364DepositorInsertcolicin E1 residues 1-364
TagsC-terminal 6x Histidine tagExpressionBacterialMutationtruncation of colicin E1 including 1-364PromoterT7Available SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRE-ChrimsonR-mCherry
Plasmid#92207PurposeReporter constructDepositorHas ServiceAAV1InsertChrimsonR-mCherry
UseAAVExpressionMammalianPromoterTREAvailable SinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXO1A
Plasmid#153141PurposeExpression of Flag tagged human FOXO1A in mammalian cellsDepositorAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_SF3B3-Xon_Luciferase
Plasmid#174659PurposePlasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.DepositorInsertXon cassette
UseLuciferaseExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC152-dual-dCas9VP64-sgExpression
Plasmid#48238PurposeDual expression construct expressing both dCas9VP64 and sgRNA from separate promotersDepositorInsertdCas9
UseCRISPRTagsHA Tag, HA-Tag, and VP64ExpressionMammalianMutationD10A;H840AAvailable SinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.iGluSnFR3.v857.PDGFR
Plasmid#178329PurposeFluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857DepositorHas ServiceAAV1InsertpAAV hSyn iGluSnFR3 v857.PDGFR
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and PDGFR TM DomainExpressionMammalianAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
SP6-VEE-GFP
Plasmid#58976Purposeexpression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA repliconDepositorInsertEGFP
UseVenezuelan equine encephalitis (vee) virus rna re…ExpressionMammalianPromoter26S subgenomic promoterAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
CCR5-donor-MCS
Plasmid#192269PurposeDonor template for CRISPR Genome EditingDepositorInsertMCS within donor template
UseCRISPRAvailable SinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP14480 - pAAV-AiE2118m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4480)
Plasmid#224046PurposeAiE2118m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in forebrain astrocytesDepositorInsertSYFP2
UseAAVMutationNAPromoterminBGAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVRc20_992
Plasmid#49739DepositorInsertAS20_992
UseSynthetic BiologyExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
[Bla][IRES][GFP]
Plasmid#97193PurposeVector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.DepositorInsertsbeta-Lactamase
GFP
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only
