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177,170 results

Showing: 10001 - 10050 of 177170 results

  1. G9A[SET]-dCas9

    Plasmid
    #100089
    Purpose
    Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G9A (EHMT2 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-G9A[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    G9A[SET] includes aa 829-1209
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100089-plasmid-map-sequence-id-197561

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  4. YIp204-PADH1-atTIR1-9myc

    Plasmid
    #99532
    Purpose
    S. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)
    Depositor
    Helle Ulrich
    Insert
    TIR1 (TIR1 Mustard Weed)
    Tags
    9xMyc
    Expression
    Yeast
    Promoter
    ADH1
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    99532-plasmid-map-sequence-id-192011

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  5. pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5

    Plasmid
    #97044
    Purpose
    Expresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.
    Depositor
    George Daley
    Publication
    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature
    Insert
    Ligand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Tet on
    Available since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    97044-plasmid-map-sequence-id-192694

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  6. AbVec2.0-IGHG2

    Plasmid
    #99576
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG2 isotype
    Depositor
    Hedda Wardemann
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    hCMV IE1 gene promoter
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    99576-plasmid-map-sequence-id-192590

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  8. pcDNA3.1-Nanoluc-ccdB

    Plasmid
    #87070
    Purpose
    Mammalian Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Tags
    Nanoluc luciferase
    Expression
    Mammalian
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    87070-plasmid-map-sequence-id-190070

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  13. pTUB1:OsTIR1-3FLAG, SAG1:CAT

    Plasmid
    #87258
    Purpose
    TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.
    Depositor
    David Sibley
    Publication
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    TIR1
    CAT
    Use
    Toxoplasma expression
    Tags
    3FLAG
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgSAG1 361 bp and TgTUB1 2717 bp
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    87258-plasmid-map-sequence-id-176556

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  14. pEGFP-C1-AR Q48

    Plasmid
    #86428
    Purpose
    Fluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region
    Depositor
    Michael Mancini
    Publication
    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Androgen receptor (AR) (AR Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Poly-glutamine expansion, G130D
    Promoter
    CMV
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86428-plasmid-map-sequence-id-378065

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  27. pMito-miRFP703

    Plasmid
    #80000
    Purpose
    Mitochondria labeling with near-infrared fluorescent protein miRFP703
    Depositor
    Vladislav Verkhusha
    Publication
    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cytochrome C oxidase subunit VIII (COX8A Human)
    Tags
    miRFP703
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    80000-plasmid-map-sequence-id-150089

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  29. pCIP

    Plasmid
    #79009
    Purpose
    For expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES.
    Depositor
    Reuben Shaw
    Publication
    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    pCAGGs
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    79009-plasmid-map-sequence-id-151714

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  33. pBluescriptSKII+ U6-sgRNA(F+E) ACTB

    Plasmid
    #74705
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA
    Depositor
    Eugene Yeo
    Publication
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    74705-plasmid-map-sequence-id-151422

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  36. R777-E285 Hs.ROCK2

    Plasmid
    #70569
    Purpose
    Gateway ORF clone of human ROCK2 [NM_004850.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ROCK2 (ROCK2 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    70569-plasmid-map-sequence-id-126018

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  40. S1PR1-Tango

    Plasmid
    #66496
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S1PR1 (S1PR1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66496-plasmid-map-sequence-id-347878

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  42. pQE-60 roGFP2-His

    Plasmid
    #65046
    Purpose
    Bacterial expression of His-tagged roGFP2
    Depositor
    Tobias Dick
    Insert
    roGFP2
    Tags
    His
    Expression
    Bacterial
    Mutation
    see sequence
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    65046-plasmid-map-sequence-id-350692

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  43. pGEM5Z(+)-EGFP

    Plasmid
    #65206
    Purpose
    EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Publication
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Restriction enzyme recognition sites added either…
    Promoter
    CMV
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    65206-plasmid-map-sequence-id-490956

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  45. pFRT-TODestFLAGHA_RNAseH1

    Plasmid
    #65782
    Purpose
    expresses FLAGHA-tagged RNAsH1 in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    RNaseH1 (RNASEH1 Human, Zebrafish)
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Promoter
    CMV, 2x Tet
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    65782-plasmid-map-sequence-id-345165

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  46. pCAG-HA-Nrxn1beta AS4(+)

    Plasmid
    #59410
    Purpose
    Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]
    Depositor
    Peter Scheiffele
    Publication
    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nrxn1beta AS4(+) (Nrxn1 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    59410-plasmid-map-sequence-id-107054

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Showing: 10001 - 10050 of 177170 results