We narrowed to 10,522 results for: SEC
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Plasmid#111930PurposeVector to express tAD candidates from a library cloned from fragmented transcription factor coding sequences fused to the Gal4 DNA binding domain.DepositorInserttAD-seq Gal4-DBD
ExpressionInsectAvailable SinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACE-GIRK2-S
Plasmid#172426PurposeExpression of full-length, human GIRK2 with C-terminal StrepTag IIDepositorAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAST-FRT-GFP-FRT-uMCas12a+nls1x
Plasmid#230908PurposeDouble inducible expression of Cas12a+ with Gal4 and Flp.DepositorInsertCas12a+
UseCRISPRExpressionInsectAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
PCYOX1L-bio-His
Plasmid#53410PurposeExpresses full-length Prenylcysteine oxidase-like precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPCYOX1L (PCYOX1L Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB[vasa-Cas9 PUb-ZsGreen]
Plasmid#169012PurposeFor germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.DepositorInsertCas9
UsePiggybacExpressionInsectPromoterDrosophila melanogaster vasaAvailable SinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL3617 (pCRISPR(BbsI)_Pse)
Plasmid#200902PurposeExpresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.DepositorInsertPseCRISPR (entry)
ExpressionMammalianAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pADH100Cau
Plasmid#244817PurposeModified plasmid from pADH100 to perform HIS-FLP CRISPR in Candidozyma aurisDepositorInsertSNR52p from C. auris
UseCRISPRTagsHIS1 terminator from C. auris and NAT resistance …ExpressionYeastPromoterSNR52pAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pC5Kan-P2A
Plasmid#51814PurposeP2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single messageDepositorInsertP2A peptide (PTV1gp1 Synthetic, porcine teschovirus-1)
UseShuttle vector for insect expression vectorsMutationcodons altered for Drosophila and to eliminate re…Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCCM’
Plasmid#162709PurposepCCM reconstructed after selection for CCMB1 growth on glycerol under ambient airDepositorInsertSecond CCM operon cloned from H. neapolitanus
ExpressionBacterialMutationp15A origin replaced with high-copy colE1 originPromoterPLtet0-1 promoterAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV5B+longBRD4
Plasmid#157792PurposeExpression of long isoform of BRD4DepositorAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDC-UAS-nBitbow1.0(miniW)
Plasmid#138329PurposeDrosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.DepositorInsertsmAmetrine
tdKatushka2
mNeonGreen
mTFP1
mKusabira-Orange2
Tagshuman H2BExpressionInsectPromoter10xUASAvailable SinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Activin A (S2-2)
Plasmid#184621Purposefor transfection into S2 cells and expression of Activin A proteinDepositorAvailable SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-11
Plasmid#72349PurposeMammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneTags6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…ExpressionMammalianPromoterCMV enhancer + chicken beta-actin promoterAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40
Plasmid#133560PurposeBackbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locusDepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-LexGAD-3xP3-RFP
Plasmid#127558PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker.DepositorInsertLexGAD
UseCRISPRExpressionInsectAvailable SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Rab10wt
Plasmid#135557PurposeExpress mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.DepositorAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1067A1
Plasmid#164847PurposeExpresses four gRNA targeting BTub gene with 3xp3-eGFP markerDepositorInsert4 gRNAs and 3xP3-eGFP
ExpressionInsectAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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OA-1067K
Plasmid#164848PurposeExpresses four gRNA targeting Mhc gene with 3xp3-tdTomato markerDepositorInsert4 gRNAs and 3xP3-tdTomato
ExpressionInsectAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-874PA
Plasmid#164846PurposeExpresses AAEL005635-Cas9 with OpIE2-CFP markerDepositorInsertP10-3'UTR OpIE2-CFP
ExpressionInsectAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHPHS800
Plasmid#228240PurposeBxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombinationDepositorInsertCas9
TagsHygromycin phosphotransferaseExpressionMammalianPromoternoneAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Gal4-3xP3-RFP
Plasmid#127556PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.DepositorInsertGal4
UseCRISPRExpressionInsectAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
SDHC-pHLuorin-sems
Plasmid#107767Purposelabeling of mitochondrial succinate dehydrogenase subunit C, pH measurementsDepositorInsertSubunit C of SDH fused to pHluorin (SDHC Human)
TagspHluorinExpressionMammalianPromoterCMVAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
Plasmid#217515PurposeEncodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD RecombinaseDepositorInsert13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
ExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA FLARE.sc Biosensor Q63L
Plasmid#12151DepositorInsertRhoA Biosensor Q63L (RHOA Human)
Tags6xHisExpressionBacterial, Insect, and Mamm…MutationRhoA Q63L (dominant-positive mutation)Available SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG94-5
Plasmid#228965PurposeFor bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaG94-5
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA FLARE.sc Biosensor T19N
Plasmid#12152DepositorInsertRhoA Biosensor T19N (RHOA Human)
Tags6xHisExpressionBacterial, Insect, and Mamm…MutationRhoA T19N (dominant-negative mutation)*Available SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-3
Plasmid#72345PurposeMammalian expression of secreted N-terminally 8His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneTags6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…ExpressionMammalianPromoterCMV enhancer + chicken beta-actin promoterAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9
Plasmid#162162PurposeExpresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)DepositorInsertshSpCas9
sgRNA
UseCRISPRTagsN/AExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mCherry-Oskar 139-240 (eLOTUS domain) (HK73)
Plasmid#206461PurposeOskar 139-240 expression in Schneider cells for imagingDepositorAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
IMPT-10316:GABR1
Plasmid#157850PurposeHA-TEV-VFT-TMD-CC-PreX-eGFP in pFastBac1 for Sf9 expressionDepositorAvailable SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaMIgG-14
Plasmid#228994PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaMIgG-14
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(D323A/D325A)
Plasmid#86592PurposeExpresses human full-length Akt1(D323A/D325A) mutantDepositorAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-3C-FIP200-GFP
Plasmid#187832PurposePlasmid for the expression and purification of GST-3C-FIP200-GFP. Internal reference: SMC1445DepositorAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFl Strep-Sumo-TEV human OAS1
Plasmid#130895PurposeHuman oligoadenylate synthase 1 (OAS1) is used to label terminal ADP-riobosDepositorAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL0828 (pQCascade_crRNA-4)
Plasmid#130637PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.3
Plasmid#190797Purposeaka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final productDepositorInsert2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
ExpressionInsectAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-U6-3-t-attP40
Plasmid#133561PurposegRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructsDepositorInsertattP40 region guide RNAs
UseCRISPRExpressionInsectPromoterU6Available SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
ppBAalbNixE1E2 PUb-GFP
Plasmid#173667Purposetransgenesis vector for Aedes albopictus ectopic expression of the Nix geneDepositorInsertsNix masculinisation gene
EGFP
ExpressionInsectMutationNix isoforms with only exons 1, 2 separated by in…PromoterAedes aegypti Polyubiquitin and cloned Nix promot…Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAEL005635-Cas9
Plasmid#100608PurposeThe promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL005635-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL005635 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only