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19,207 results

Showing: 10151 - 10200 of 19207 results

  1. pDG462

    Plasmid
    #100903
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100903-plasmid-map-sequence-id-196607

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  5. RP4-8

    Plasmid
    #79813
    Purpose
    Modified broad host range plasmid RP4. Gentamicin resistant. Helper plasmid for conjugation
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Gentamicin resistance gene
    Use
    Helper bhr plasmid for mobilizing other plasmids …
    Tags
    Expression
    Mutation
    Promoter
    Own promoter
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. pFastBac_Dual_SNF2L-opt_p48

    Plasmid
    #102243
    Purpose
    Expression of human codon optimized human SNF2L and pRbAp48 from pFastBac dual
    Depositor
    Joe Landry
    Inserts
    SNF2L (SMARCA1 Human)
    pRbAp48 (RBBP4 Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    codon optimized for expression
    Promoter
    Polyhedrin and p10
    Available since
    Nov. 1, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    102243-plasmid-map-sequence-id-197769

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  12. pCAPB02

    Plasmid
    #65628
    Purpose
    A transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilis
    Depositor
    Pei-yuan Qian
    Article
    Li et al Sci Rep. 2015 Mar 24;5:9383. doi: 10.103
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    65628-plasmid-map-sequence-id-120067

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  21. msfGFP-Cx43

    Plasmid
    #69024
    Purpose
    Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx43 (Gja1 Rat)
    Use
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    69024-plasmid-map-sequence-id-127982

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  22. pVITRO1-M80-F2-IgG1/κ

    Plasmid
    #50383
    Purpose
    Expresses human IgG1/κ antibody isotype with unknown specificity. Useful vector for cloning of human antibodies with kappa light chain
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    human Gamma 1 heavy chain expression cassette with unknown specificity
    human Kappa light chain expression cassette with unknown specificity
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available since
    Jan. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    50383-plasmid-map-sequence-id-75289

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  39. pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro

    Plasmid
    #141371
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Article
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp5-C145A (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    C145A, human codon optimized
    Promoter
    EF1alpha
    Available since
    April 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141371-plasmid-map-sequence-id-288079

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  41. FRP2375

    Plasmid
    #127581
    Purpose
    Tagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and NAT resistance gene
    Depositor
    Joerg Stelling
    Article
    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1
    Insert
    PtetO7.1_Flagtag_8Glycine linker
    Use
    Synthetic Biology; Pcr based tagging
    Tags
    Flag tag, 8glycine linker
    Expression
    Mutation
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    127581-plasmid-map-sequence-id-252229

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  45. mCherry-Alpha-Actinin-19

    Plasmid
    #54975
    Purpose
    Localization: Alpha Actinin, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    actinin alpha 1 (ACTN1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    54975-plasmid-map-sequence-id-152016

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Showing: 10151 - 10200 of 19207 results