Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

177,170 results

Showing: 10401 - 10450 of 177170 results

  5. pAcGP67A - murine TfR

    Plasmid
    #12392
    Depositor
    Pamela Bjorkman
    Insert
    transferrin receptor (Tfrc Mouse)
    Tags
    6X-His tag, Factor Xa cleavage site, and leader p…
    Expression
    Insect
    Mutation
    See comments section
    Available since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    12392-plasmid-map-sequence-id-274275

    Enlarge

  7. pRS418

    Plasmid
    #11256
    Depositor
    Fred Cross
    Type
    Empty backbone
    Expression
    Yeast
    Available since
    Feb. 15, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    11256-plasmid-map-sequence-id-6930

    Enlarge

  15. TRIPZ-bioGEF-UBnc-PURO

    Plasmid
    #208044
    Purpose
    Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Publication
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    UBnc (UBC Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    208044-plasmid-map-sequence-id-410266

    Enlarge

  18. pcDNA3.3_SARS2_XBB.1.5

    Plasmid
    #196585
    Purpose
    expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (XBB.1.5)
    Expression
    Mammalian
    Mutation
    G252V, F486P on the basis of XBB
    Promoter
    CMV
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    196585-plasmid-map-sequence-id-386779

    Enlarge

  19. pGL4_CRE-CMVmin-luc2

    Plasmid
    #194384
    Purpose
    Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter
    Depositor
    Michael Wehr
    Publication
    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    6xCRE-CMVmin-luc2
    Expression
    Mammalian
    Promoter
    CMV minimal
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    194384-plasmid-map-sequence-id-381615

    Enlarge

  26. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid
    #183409
    Purpose
    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells
    Depositor
    Azim Surani
    Publication
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183409-plasmid-map-sequence-id-361258

    Enlarge

  36. pMAH-POLY-eRF1(E55D)

    Plasmid
    #157925
    Purpose
    Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells
    Depositor
    Huiwang Ai
    Publication
    Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-
    Insert
    POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    157925-plasmid-map-sequence-id-310653

    Enlarge

  38. pCAG R2pH-LAMP1-3xFLAG

    Plasmid
    #157940
    Purpose
    expresses ratiometric sensor of lysosomal lumenal pH
    Depositor
    Massimiliano Stagi
    Publication
    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
    Tags
    3XFLAG, mCherry, and pHluorin
    Expression
    Mammalian
    Promoter
    Chick beta actin pomoter
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    157940-plasmid-map-sequence-id-315065

    Enlarge

  40. pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

    Plasmid
    #154786
    Purpose
    Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE
    Depositor
    Robert Singer
    Publication
    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:
    Insert
    Adenosine deaminse (Adar Fly, Synthetic)
    Use
    Lentiviral
    Tags
    GFP, V5, and mCherry
    Expression
    Mammalian
    Mutation
    Synonymized serine to prevent autoediting of ADAR…
    Promoter
    UBC
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    154786-plasmid-map-sequence-id-306310

    Enlarge

Showing: 10401 - 10450 of 177170 results