162,717 results

1. pMpGWB301

   Plasmid

   #68629

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TFORF0833

   Plasmid

   #141726

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   GTF2F2 (GTF2F2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AMPK(T>A)-SPARK

   Plasmid

   #226256

   Purpose

   phospho-null mutant of AMPK-SPARK

   Depositor

   Roland Malli

   Article

   Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

   Insert

   AMPK(T>A)-SPARK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   substituted Threonine 20 with Alanine

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBABE myc-Raptor

   Plasmid

   #18116

   Depositor

   Reuben Shaw

   Article

   Gwinn et al Mol Cell. 2008 Apr 25. 30(2):214-26.

   Insert

   Raptor (RPTOR Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

5. LCV2_LacZ_sgRNA_2

   Plasmid

   #155093

   Purpose

   lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   LacZ_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-H2B-Venus

   Plasmid

   #87614

   Purpose

   Venus (YFP) fused to histone H2B.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   H2B-Venus (H2BC8 Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAGM4723:Ble:Cas9

   Plasmid

   #112208

   Purpose

   Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.

   Depositor

   Thomas Mock

   Inserts

   Cas9
   SheBle
   

   Use

   CRISPR; Diatom, phaeodactylum

   Tags

   Phaeodactylum fcp promoter and terminator and YFP

   Expression

   Mutation

   Phaeodactylum Fcp promoter

   Promoter

   Phaeodactylum Fcp

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HA-MBP-TEVpCS-ADGRE3 CTFdeltaTA-FLAG

   Plasmid

   #217724

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE3 (ADGRE3 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-344

   Promoter

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA-MBP-TEVpCS-ADGRE2 CTFdeltaTA-FLAG

   Plasmid

   #217723

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE2 (ADGRE2 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-523

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CAG-mCardinal

    Plasmid

    #197195

    Purpose

    Expresses the protein of mCardinal in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    mCardinal
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p3998 pGEX HHR23A

    Plasmid

    #10864

    Depositor

    Peter Howley

    Article

    Kumar et al J Biol Chem. 1999 Jun 25. 274(26):18785-

    Insert

    HHR23A (RAD23A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pXW109Hg(RS-RinA-E11)2

    Plasmid

    #123151

    Purpose

    Mercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, PmerT, PhrpLE, PrinA

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3puro-EGFP-GAPDH

    Plasmid

    #225475

    Purpose

    Express mEGFP-fusion protein of GAPDH

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    GAPDH (GAPDH Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET11a_3xFlag-nsp14 (SARS-CoV-2)

    Plasmid

    #169159

    Purpose

    To express SARS-CoV-2 nsp14 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tubulin-LSSmGFP

    Plasmid

    #186535

    Purpose

    Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to tubulin

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Use

    Tags

    Tubulin

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GPR77-Tango

    Plasmid

    #66373

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR77 (C5AR2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. TFORF1569

    Plasmid

    #141841

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF680 (ZNF680 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GLE1

    Plasmid

    #155625

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    GLE1 (GLE1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HMGA2+GFP

    Plasmid

    #25405

    Depositor

    Sean Morrison

    Article

    Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

    Insert

    high mobility group AT-Hook 2 (Hmga2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    polyadenylation signal lacking

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pCI-Myc3-HPS4

    Plasmid

    #133931

    Purpose

    Expresses 3xMyc-HPS4 construct in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Martina et al J Biol Chem. 2003 Aug 1;278(31):29376-84

    Insert

    HPS4 (HPS4 Human)
    

    Use

    Tags

    3xMyc tag

    Expression

    Mammalian

    Mutation

    Glutamic acid 229 to Glycine; Valine 552 to Methi…

    Promoter

    CMV I.E.

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TFORF1512

    Plasmid

    #144366

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NKX3-2 (NKX3-2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEF-BOS-T7-CA150

    Plasmid

    #21924

    Depositor

    Mariano Garcia-Blanco

    Article

    Sune et al Mol Cell Biol. 1999 Jul . 19(7):4719-28.

    Insert

    CA150 (TCERG1 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

23. C1ORF43-3xFLAG

    Plasmid

    #130880

    Purpose

    Expresses human C1ORF43 with C-terminal 3xFLAG tag

    Depositor

    Michael Bassik

    Article

    Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

    Insert

    C1ORF43 (C1orf43 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TFORF0677

    Plasmid

    #142192

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PRDM16 (PRDM16 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBABE myc-Raptor S722A/S792A

    Plasmid

    #18117

    Depositor

    Reuben Shaw

    Article

    Gwinn et al Mol Cell. 2008 Apr 25. 30(2):214-26.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S722A, S792A

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

26. pMpGWB326

    Plasmid

    #68654

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLJM60-Ptb

    Plasmid

    #38245

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Ptb (Ptbp1 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJMP1159

    Plasmid

    #119250

    Purpose

    rfp "test" strain

    Depositor

    Jason Peters, Carol Gross, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRS41K-mCherry-sfGFP (pJJF001)

    Plasmid

    #173457

    Purpose

    C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HMGA2(3'UTR DEL)+GFP

    Plasmid

    #25406

    Depositor

    Sean Morrison

    Article

    Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

    Insert

    high mobility group AT-Hook 2 (Hmga2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    3" UTR is absent

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

31. TFORF3023

    Plasmid

    #144499

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NEUROD6 (NEUROD6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDONR221-Hyg

    Plasmid

    #48994

    Purpose

    Gateway adapted transfer of Hygromycin resistance gene

    Depositor

    Rick Tarleton

    Article

    Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

    Insert

    hygromycin B phosphotransferase
    

    Use

    Gateway attb1 & attb2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. HMGA2 shRNA

    Plasmid

    #25408

    Depositor

    Sean Morrison

    Article

    Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

    Insert

    high mobility group AT-Hook 2 (Hmga2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

34. plVX-EF1a-FLAG-hMETTL3-IRES-ZsGreen

    Plasmid

    #207123

    Purpose

    Lentiviral vector to express human METTL3

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    hMETTL3 (METTL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. HBT-CPK32-Flag

    Plasmid

    #107432

    Purpose

    For Arabidopsis mesophyll protoplast transient expression assay

    Depositor

    Jen Sheen

    Article

    Liu et al Nature. 2017 May 18;545(7654):311-316. d

    Insert

    CPK32 (CPK32 Mustard Weed)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP

    Plasmid

    #121448

    Purpose

    To make a stable human recombinant cell line; see right thumbnail map image for PQR annotations

    Depositor

    Brian Chen

    Article

    Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

    Insert

    RFPnols-PQR-ZeocinR (ACTB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TFORF1800

    Plasmid

    #143546

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    USF2 (USF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TFORF2884

    Plasmid

    #143654

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FIGLA (FIGLA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Kohinoor-Vimentin/pcDNA3

    Plasmid

    #67772

    Purpose

    Vimentin-targeted Kohinoor for mammalian cell expression

    Depositor

    Takeharu Nagai

    Article

    Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

    Insert

    Kohinoor-Vimentin
    

    Use

    Tags

    Vimentin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

40. BMPR2

    Plasmid

    #38972

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BMPR2 (BMPR2 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only