173,273 results
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hGli3 flag3x
Plasmid#84921Purposemammalian expression of Gli3DepositorAvailable SinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Human SERCA2a (pcDNA3.1+)
Plasmid#75187PurposeMammalian expression of ATP2A2DepositorAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRpHLuorin2-N1
Plasmid#171716PurposeRatiometric pHLuorin2 backbone (C terminal fusion)DepositorTypeEmpty backboneExpressionMammalianAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
CCR6-DuET
Plasmid#213202PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 TDP-43 YFP
Plasmid#84911Purposeexpression of TDP-43 YFP in mammalian cellsDepositorAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO eGFP Puro (w159-1)
Plasmid#17481Purpose3rd gen lentiviral eGFP expression vector, CMV/TO promoter, PuroDepositorInsertenhanced green fluorescent protein
UseLentiviralExpressionMammalianAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR
Plasmid#82024PurposeLentiviral construct for building stable cell lines expressing mKate2 in the presence of TNFa via puromycin selectionDepositorInsertmKate2-2xNLS
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE
Plasmid#89609Purposeover-expression of VEGF-A(165) in human cellsDepositorAvailable SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
TMED10-bio-His
Plasmid#51852PurposeExpresses full-length Transmembrane emp24 domain-containing protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTMED10 (TMED10 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Px461-Cas9n-Trp53-sgRNA-beta
Plasmid#88847PurposeCRISPR KO of Trp53DepositorAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
His6TEV-UbG76C
Plasmid#75299PurposeBacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV proteaseDepositorInserthuman Ubiquitin mutant G76 to C
TagsHisExpressionBacterialMutationchanged Glycine 76 to CystinePromoterT7Available SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SFPQ
Plasmid#166960PurposeExpresses FLAG-tagged SFPQ protein in pcDNA3.1DepositorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Hygro DHFR.cHSF1
Plasmid#134738PurposeLentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domainDepositorInsertDHFR.cHSF1 (HSF1 Human)
UseLentiviralTagsDHFRExpressionMammalianMutationDeletion of amino acids 186-202PromoterCMVAvailable SinceOct. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-A3-HA-ubiquitin
Plasmid#220145PurposeExpresses A3-Ub for E3 substrate identification by E-STUBDepositorAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-anti-mCherry(LaM6) PAGER(Gs)
Plasmid#230017PurposeLentiviral vector expressing anti-mCherry PAGER(Gs) in mammalian cellsDepositorInsertMT1-LaM6-TEVcs-ALFA-hM1Dxs
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
eGFP STAT1 Y701F
Plasmid#12302DepositorAvailable SinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-PM-TurboID-V5_IDG-K
Plasmid#214455PurposeGateway destination clone of PM-TurboID (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertPM-TurboID-V5
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianPromoterUbiquitinAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOP01-UTC-7.0
Plasmid#216625PurposeNanoLuc reporter plasmid to optimize chloroplast cell-free systems, universal test constructDepositorInsertT7p-5'g10-NanoLuc-3'TMV
UseSynthetic BiologyPromoterT7Available SinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
2XSmBiT-SARS-CoV-2 S1
Plasmid#173430Purposeprotein expression plasmid of 2XSmBiT-SARS-CoV-2 S1-IgG1 FcDepositorInsert2XSmBiT SARS-CoV-2 S1-IgG1 Fc (S SARS-CoV-2)
UseLuciferaseTagshuman IgG1 FcExpressionMammalianMutationfurin cleavage site RRAR to GSASPromoterCMVAvailable SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pConRef-2H12.D11
Plasmid#221151PurposecdGreen2 expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP Internal Lab ID: 11236)DepositorInsertscdGreen2
mScarlet-I
ExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterConstitutive and None (same as cdGreen2)Available SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-Hyg-CTCF-N1
Plasmid#131799PurposeExpresses human CTCF-GFP in mammalian cells through transient transfectionDepositorAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
PKD1-F (pCI-PKD1-Flag)
Plasmid#21369DepositorInsertautosomal dominant polycystic kidney disease type I (PKD1 Human)
TagsFLAGExpressionMammalianMutationwild-type sequence*Available SinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-IKBalpha-mut (super repressor)
Plasmid#15291DepositorInsertinhibitor of Kappa B alpha (NFKBIA Human)
UseRetroviralExpressionMammalianMutationS32A/S36A: “super-repressor” allele (resistant to…Available SinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
YY206: pAAV_hSyn::GEMINI(B)-p2A-GEMINI(A)_UbC::HaloTag-GEMINI(A)
Plasmid#228886PurposeAAV plasmid expressing the two GEMINI building blocks (A and B) in an equimolar ratio under the control of a hSyn promoter, and HaloTag-GEMINI(A) under the control of a UbC promoter.DepositorInsertGEMINI(A); GEMINI(B); HaloTag-GEMINI(A)
UseAAVTagsHaloTagExpressionMammalianPromoterhSyn; UbcAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA Arf1 ActQ71L
Plasmid#10832DepositorInsertArf1 Q71L (ARF1 Human)
TagsHAExpressionMammalianMutationQ71L. Defective in GTP hydrolysis. Constitutive…Available SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Fos DD
Plasmid#8968DepositorAvailable SinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
Cys-3HB-SPYcatcher
Plasmid#214838PurposeThis plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-3HB-SPYcatcher
TagsHis-TEV-SUMO-ExpressionBacterialPromoterT5Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0021
Plasmid#141480PurposeLentiviral vector for overexpressing the IRX1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLC-284
Plasmid#124294PurposeA low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.DepositorInsertvsfGFP-9
ExpressionBacterialAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ETF1-E55D
Plasmid#130876PurposeEncodes ETF1 with an E55D substitutionDepositorInsertEukaryotic transcritional termination factor ETF1-E55D (ETF1 Human)
ExpressionMammalianMutationE55DPromoterCAGAvailable SinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-Fzd4 subtype NGS Wnt
Plasmid#159625PurposeExpress Fzd4 subtype NGS Wnt in mammalian cellsDepositorInsertFzd4 subtype NGS Wnt
UseLentiviralTagsHis tagExpressionMammalianAvailable SinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
JD689
Plasmid#160396Purposecitrus GRF4-GIF1 in pDONR-zeoDepositorInsertcitrus GRF4-GIF1 chimera
UseEntry clonAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-Sestrin1 isoform1
Plasmid#72594Purposeoverexpression, codon optimized for mammalian expressionDepositorInsertSESN1 (SESN1 Human)
TagsFLAGExpressionMammalianMutationcodon optimized for mammalian expression, see dep…PromoterCMVAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-ACTB
Plasmid#207748PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.DepositorAvailable SinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMal-Strep
Plasmid#209345PurposeProtein expression vector with a TEV cleavable N-terminal 6xHis-MBP and C-terminal StrepTagIIDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
mPlum-Lysosomes-20
Plasmid#55982PurposeLocalization: Lysosome Membrane, Excitation: 590, Emission: 649DepositorAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPB-PGK-destination
Plasmid#60436PurposeDestination vector with PGK - Hygromycin selection markerDepositorTypeEmpty backboneExpressionMammalianAvailable SinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL2-NOS2Promoter-Luciferase
Plasmid#19296DepositorAvailable SinceNov. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBAD42-BtuCEDFB
Plasmid#237541Purposeplasmid encodes BtuC, BtuD, BtuE, BtuF, BtuB for enhanced solubilization of cobalamin proteinsDepositorInsertsbtuB
btuF
btuCED
ExpressionBacterialPromoterpBADAvailable SinceSept. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Puro
Plasmid#167911PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a DIO-rsChRmine-oScarlet-Kv2.1-WPRE
Plasmid#183529PurposeOptogeneticsDepositorInsertrsChRmine-oScarlet-Kv2.1
UseAAVMutationI146M/G174SPromoterEf1aAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-P2A-CFP no-pA
Plasmid#80827PurposeP2A followed by CFP without pADepositorInsertp3E-P2A-CFP no pA
ExpressionMammalianAvailable SinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-24xGCN4_v4-BFP-24xPP7
Plasmid#74929PurposeTranslation reporterDepositorInsert24xGCN4_v4-BFP-24xPP7
ExpressionMammalianAvailable SinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR4-YFP
Plasmid#13018DepositorAvailable SinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Lifeact-EGFP-2XNLS
Plasmid#58467PurposeExpresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoterDepositorInsertsLifeact
2X NLS with 2X stop codons
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME pHluorin2
Plasmid#73794Purposeratiometric pHluorin2 sequence in a middle entry cloneDepositorInsertratiometric pHluorin2
UseMiddle entry cloneAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSIG1
Plasmid#226908PurposeMoClo-compatible vectors for dsRNA production in E. coliDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7 promoterAvailable SinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only
