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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 11,735 results for: pcDNA

Showing: 11051 - 11100 of 11735 results

  2. FKBP-DCRD-YFP

    Plasmid
    #87450
    Purpose
    rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels
    Depositor
    Xiaodong Liu
    Article
    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1
    Insert
    DCRD (distal C-terminal regulatory domain) (CACNA1F Human)
    Use
    Tags
    6xHis-T7, FKBP, and YFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87450-plasmid-map-sequence-id-174914

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  3. Epac-S-H96

    Plasmid
    #170342
    Purpose
    CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.
    Insert
    CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170342-plasmid-map-sequence-id-344641

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  5. LIC-Z-YFP

    Plasmid
    #153544
    Purpose
    perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication
    Depositor
    Katharina Gaus
    Article
    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:
    Insert
    human TCR ζ-Chain (CD247 Human)
    Use
    Tags
    Venus YFP
    Expression
    Mammalian
    Mutation
    with mCherry in LIC-Z replaced by YFP
    Promoter
    CMV
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    153544-plasmid-map-sequence-id-305908

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  10. CFP-PCRD-FRB

    Plasmid
    #87449
    Purpose
    rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels
    Depositor
    Xiaodong Liu
    Article
    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1
    Insert
    PCRD (proximal C-terminal regulatory domain) (CACNA1F Human)
    Use
    Tags
    6xHis-T7, CFP, and FRB
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87449-plasmid-map-sequence-id-174912

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  11. pRD439

    Plasmid
    #168465
    Purpose
    For the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS-mTurquoise2-H-NSdbd-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168465-plasmid-map-sequence-id-331915

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  12. AIDx-ID-1058

    Plasmid
    #135347
    Purpose
    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminus
    Depositor
    Alex Hewitt
    Article
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Insert
    nSpyCas9-hAIDx-ID-1058-UGI
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135347-plasmid-map-sequence-id-267696

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  27. pKK-mVenus-TEV

    Plasmid
    #105774
    Purpose
    Expression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mVenus-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105774-plasmid-map-sequence-id-203363

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  39. pAK208_2xBRD9BD-IFP2.0C

    Plasmid
    #233734
    Purpose
    Expresses BRD9 Bromodomain as tandem double domain fused to C-terminal part of IFP2.0 for histone acetylation detection in BiAD sensors
    Depositor
    Albert Jeltsch
    Article
    Köhler et al Genes (Basel). 2025 Apr 10;16(4):444. do
    Insert
    BRD9 (BRD9 Human)
    Use
    Tags
    IFP2.0 C-terminal part
    Expression
    Mammalian
    Mutation
    Bromodomain cloned twice in tandem
    Promoter
    CMV
    Available since
    May 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233734-plasmid-map-sequence-id-472157

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  40. pAK208_2xBRD9BD_Y57A-IFP2.0C

    Plasmid
    #233733
    Purpose
    Expresses BRD9 Bromodomain as tandem double domain Y57A mutant fused to C-terminal part of IFP2.0 as negative control for plasmid 233734
    Depositor
    Albert Jeltsch
    Article
    Köhler et al Genes (Basel). 2025 Apr 10;16(4):444. do
    Insert
    BRD9 (BRD9 Human)
    Use
    Tags
    IFP2.0 C-terminal part
    Expression
    Mammalian
    Mutation
    Bromodomain cloned twice in tandem with Y57A muta…
    Promoter
    CMV
    Available since
    May 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233733-plasmid-map-sequence-id-472158

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  41. pVEA3-KNC

    Plasmid
    #234801
    Purpose
    Mammalian expression of bovine arrestin-3 (K11A, K12A, L49A, D51A, R52A, L69A, Y239A, D241A, C252A, P253A, D260A, Q262A)
    Depositor
    Vsevolod Gurevich
    Article
    Breitman et al J Biol Chem. 2012 Jun 1;287(23):19653-64
    Insert
    Arrestin-3 (ARR3 Bovine)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    K11A, K12A, L49A, D51A, R52A, L69A, Y239A, D241A,…
    Promoter
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    234801-plasmid-map-sequence-id-469807

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Showing: 11051 - 11100 of 11735 results