We narrowed to 12,055 results for: cel.2

1. pMXs-INL (with mNgn2x3HA)

   Plasmid

   #233197

   Purpose

   Retroviral expression of Isl1, Ngn2x3HA, and Lhx3 for motor neuron reprogramming

   Depositor

   Kate Galloway

   Article

   Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

   Insert

   Isl1-P2A-mNgn2x3HA-T2A-mLhx3 (Neurog2, Isl1, Lhx3 Mouse)
   

   Use

   Retroviral

   Tags

   x3HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXS-IRES-BLAST flag-CAD hCPS1 TL

   Plasmid

   #188150

   Purpose

   Expresses C-terminal flag-tagged human CAD hCPS1 TL in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

3. pMIG-hNFATc2/C(1-686)AVITEV

   Plasmid

   #74054

   Purpose

   retroviral expression plasmid for human NFATc2/C (without C-terminal region) with C-terminal BirA-biotinylation signal and TEV celavage site

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   human NFATc2, isoform C (NFATC2 Human, AS 1-686 (N terminus deleted))
   

   Use

   Retroviral

   Tags

   AVI-TEV

   Expression

   Mammalian

   Mutation

   silent mutation A1723C in the sequence of NM_1730…

   Promoter

   pMSCV-LTRs

   Available Since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-EF1-mVenus-p27K−

   Plasmid

   #176651

   Purpose

   It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).

   Depositor

   Mohamed Bentires-Alj

   Article

   Correia et al Nature. 2021 Jun;594(7864):566-571. doi:

   Insert

   mVenus-p27K- (Cdkn1b Mouse)
   

   Use

   Lentiviral

   Tags

   mVenus

   Expression

   Mutation

   Mutant K- (lacks affinity to Cdk)

   Promoter

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMIG-hNFATc2/C(RIT)-FLAG-AVITEV

   Plasmid

   #74053

   Purpose

   retroviral expression plasmid for human NFATc2/C (with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   human NFATc2, isoform C (NFATC2 Human)
   

   Use

   Retroviral

   Tags

   AVI-TEV and FLAG

   Expression

   Mammalian

   Mutation

   silent mutation A1723C in the sequence of NM_1730…

   Promoter

   pMSCV-LTRs

   Available Since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSC-NGN2-IRES-ISL1-T2A-LHX3

   Plasmid

   #221814

   Purpose

   Lentiviral vector expressing human NEUROG2, ISL1 and LHX3 for inducing pluripotent stem cells into cholinergic motor neurons.

   Depositor

   Baojin Ding

   Article

   Sepehrimanesh et al Am J Physiol Cell Physiol. 2020 Oct 1;31

   Inserts

   NEUROG2 (NEUROG2 Human)
   ISL1 (ISL1 Human)
   LHX3 (LHX3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIG-828_HA-GD2-28z_CAR_BATF_Retroviral

   Plasmid

   #207505

   Purpose

   This plasmid can be used to generate retrovirus.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   BATF, HA-GD2-28z_CAR (BATF Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Phage-PGK-Flag-HA-FKBP-T(G177D)

   Plasmid

   #181735

   Purpose

   for lentiviral expression of brachyury ORF in mammalian cells

   Depositor

   Charles Lin

   Article

   Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

   Insert

   T (brachyury)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   a silent mutation in the PAM site of an sgRNAt ta…

   Promoter

   Available Since

   March 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pMXs-Sox2-17

   Plasmid

   #193353

   Purpose

   Retroviral vector expressing mouse Sox2-17 chimeric transcription factor (super-Sox)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Sox2-17 (Sox2 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Sox2-17 is engineered highly cooperative chimeric…

   Promoter

   5'LTR

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD649-HAsp-PSG1-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156815

    Purpose

    Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    PSG1 (PSG1 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE2-TetOminiCMV-OSK

    Plasmid

    #136552

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-P2A-Sox2-T2A-Klf4 (Klf4, Sox2, Pou5f1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE2-TetOminiCMV-SKM

    Plasmid

    #136551

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Sox2, Klf4, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2-T2A-Klf4-E2A-cMyc (Sox2, Klf4, Myc Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRE3G-GSX2-IRES2-EBF1

    Plasmid

    #96966

    Purpose

    Dox-inducible expression of Gsx2 and Ebf1 in mammalian cells

    Depositor

    Elena Cattaneo

    Article

    Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    GSX2 and EBF1 (bicistronic insert) (Gsx2, Ebf1 Mouse)
    

    Use

    Tags

    FLAG (on EBF1)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE2-TetOminiCMV-KSM

    Plasmid

    #136553

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Klf4, Sox2, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Klf4-IRES-Sox2-E2A-cMyc (Klf4, Sox2, Myc Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHAGE2-TetOminiCMV-OSM

    Plasmid

    #136555

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-P2A-Sox2-E2A-cMyc (Myc, Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB513B-1/TRE-hHNF1β-hHNF4α-hHNF6-EF1α-Bla

    Plasmid

    #199551

    Purpose

    PiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1β-hHNF4α-hHNF6) under control of the TRE/PCMVmin promoter

    Depositor

    Masamichi Kamihira

    Article

    Kitano et al Cells. 2022 Apr 1;11(7):1194. doi: 10.33

    Insert

    hHNF1β-hHNF4α-hHNF6 (ONECUT1, HNF4A, HNF1B Human)
    

    Use

    Piggybac transposon vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE+CMVmin promoter

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV_BiPVe4_ChR2_mCherry

    Plasmid

    #213937

    Purpose

    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_ChR2_mCherry

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Has Service

    AAV PHP.eB and AAV1

    Insert

    Channelrhodopsin 2-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV_BiPVe3_ChR2_mCherry

    Plasmid

    #213941

    Purpose

    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer. Alias: AAV_WDP0003_ChR2_mCherry

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Has Service

    AAV PHP.eB and AAV1

    Insert

    Channelrhodopsin 2-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLX_lenti_osTir1_9Myc_P2A_Bsd

    Plasmid

    #139746

    Purpose

    lentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection marker

    Depositor

    Robert Tjian

    Article

    Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

    Insert

    rice E3 ligase Tir1 (osTir1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-flag-YTHDF2

    Plasmid

    #52300

    Purpose

    full length YTHDF2 with N-terminal flag tag for mammalian cell expression

    Depositor

    Chuan He

    Article

    Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GFP-cdc42palm

    Plasmid

    #20141

    Depositor

    Alaa El-Husseini

    Article

    Kang et al Nature. 2008 Dec 18. 456(7224):904-9.

    Insert

    cell division cycle 42 isoform 2 (CDC42 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

22. iSM_PPL2-IP

    Plasmid

    #235989

    Purpose

    Expresses iSM_PPL2 in mammalian cells

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

    Insert

    iSM_PPL2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    replacement of 5 amino acids of Smac-Cas9 PL2 wit…

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pYO1124

    Plasmid

    #235725

    Purpose

    Expression of human UVRAG (1-464) fused to human ATG14L BATS (413-492) in mammalian cells

    Depositor

    Roger Williams

    Article

    Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

    Insert

    UVRAG(1-464) fused to ATG14L BATS domain (413-492)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOD2045-intControl

    Plasmid

    #100005

    Purpose

    MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2044-intDEG. Expression is controlled by Pelt-2 (intestine specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Inserts

    zif-1 (zif-1 Nematode)
    unc-119
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDH-puro-Bcl2

    Plasmid

    #46971

    Purpose

    lentiviral expression of Bcl2

    Depositor

    Jialiang Wang

    Article

    Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

    Insert

    Bcl2 (BCL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-FLAG-puro-PTPIP51(1-470)

    Plasmid

    #170536

    Purpose

    Expresses PTPIP51(1-470) in human HeLa cells after transfection

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S

    Plasmid

    #166794

    Purpose

    Mammalian cell expression vector for Flag-tagged DPP9S

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6

    Plasmid

    #177849

    Purpose

    Bacterial Expression of PNR

    Depositor

    Karsten Melcher

    Article

    Tan et al PLoS One. 2013 Sep 12;8(9):e74359. doi:

    Insert

    photoreceptor cell-specific nuclear receptor (NR2E3 Human)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-HA-eEF2K

    Plasmid

    #110160

    Purpose

    Expression of human eEF2K in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Kruiswijk et al Sci Signal. 2012 Jun 5;5(227):ra40. doi:

    Insert

    eEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBC2T-LC3B

    Plasmid

    #112026

    Purpose

    expresses BC2-tag-LC3B in mammalian cells

    Depositor

    Ulrich Rothbauer

    Article

    Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Tags

    BC2-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNAZeo(-) LAMP2C

    Plasmid

    #89342

    Purpose

    Expresses human LAMP-2C in mammalian cells

    Depositor

    Janice Blum

    Article

    Perez et al J Immunol. 2016 Mar 15;196(6):2457-65. d

    Insert

    Lysosomal-associated membrane protein 2C (LAMP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pClneoFLAG human Dendrin

    Plasmid

    #47934

    Purpose

    expresses FLAG tagged human Dendrin in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Kawata et al J Biochem. 2006 May;139(5):931-9.

    Insert

    Dendrin-1 (DDN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    contains amino acids 2-711 of human Dendrin

    Promoter

    CMV

    Available Since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

33. pQCXIP GFP-MOSPD2 RD/LD

    Plasmid

    #186468

    Purpose

    Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to EGFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R404D/L406D (mutant unable to bind FFAT motifs)

    Promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e

    Plasmid

    #90010

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Cdc25C WT
    NBirA*-14-3-3e
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1 LIC 6A HIS-TEV-DPP9S

    Plasmid

    #166791

    Purpose

    Mammalian cell expression vector for His-tagged DPP9S

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pX330-NL-DHFR-SpCas9

    Plasmid

    #124522

    Purpose

    Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

    Insert

    NL-DHFR-SpCas9
    

    Use

    AAV

    Tags

    Destabilized domain of E.coli dihydrofolate reduc…

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBC2T-lamin

    Plasmid

    #108972

    Purpose

    expresses BC2-tag-lamin in mammalian cells

    Depositor

    Ulrich Rothbauer

    Article

    Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

    Insert

    lamin B1 (LMNB1 Human)
    

    Use

    Tags

    BC2-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFastBac HTB DPP9L

    Plasmid

    #166788

    Purpose

    Insect cell expression vector for DPP9L (protein purification)

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFastBac HTB DPP9S

    Plasmid

    #166787

    Purpose

    Insect cell expression vector for DPP9S (protein purification)

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFastBac HTB DPP9S S730A

    Plasmid

    #169946

    Purpose

    Purification of catalytically-dead DPP9S from insect cells

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Insect

    Mutation

    S730A

    Promoter

    polyhedrin

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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41. pFastBac HTB DPP9S D808N

    Plasmid

    #169947

    Purpose

    Purification of catalytically-dead DPP9S from insect cells

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Insect

    Mutation

    D808N

    Promoter

    polyhedrin

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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42. pCSIIbleo-PIF3-mEGFP

    Plasmid

    #139483

    Purpose

    Expresses PIF3-mEGFP in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    PIF3 (PIF3 Mustard Weed)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    PIF3 1-100 aa

    Promoter

    Human EF1a promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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43. pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

    Plasmid

    #90011

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Cdc25C S216A
    NBirA*-14-3-3e
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Ser 216 to Ala

    Promoter

    CMV

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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44. eSpCas9-EF-5Usp5

    Plasmid

    #174873

    Purpose

    CRISPR vector for generating Usp5 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Usp5 (Usp5 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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45. eSpCas9-EF-5Wnk1

    Plasmid

    #174874

    Purpose

    CRISPR vector for generating Wnk1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Wnk1 (Wnk1 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pcDNA-FLAG-Co-NF-kB-Cterm

    Plasmid

    #180199

    Purpose

    Expresses FLAG-tagged Capsaspora NF-kB C-terminal sequences in mammalian cells

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    Co-NF-kBCterm
    

    Use

    Tags

    FLAG-tagged fusion protein

    Expression

    Mammalian

    Mutation

    aa 542-1224

    Promoter

    CMV

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. Halo-WIPI2B H85E K88E I92E

    Plasmid

    #175033

    Purpose

    Expresses WIPI2B (H85E K88E I92E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

    Depositor

    Andrea Stavoe

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2B (H85E K88E I92E) (WIPI2 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Histadine 85 to Glutamate (CAC to GAG); Lysine 88…

    Promoter

    CMV

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. FLAG-HA-RNF68 [dRING]

    Plasmid

    #126549

    Purpose

    Expresses FLAG-HA-tagged RNF68 [dRING mutant] in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RNF68 (PCGF1 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    RING domain deletion mutant [dRING mutation is a …

    Promoter

    LTR

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pcDNA4/TO h-Stx3S-2xMyc/His

    Plasmid

    #99744

    Purpose

    Expresses human Stx3S with a C-term myc-myc-his tag in mammalian cells.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

    Insert

    Stx3S (STX3 Human)
    

    Use

    Tags

    myc-myc-his

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. pMCh-1539

    Plasmid

    #82432

    Purpose

    Plasmid for expression of GST-tagged human-Nematostella GW182 chimera in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    hs-nvGW182 chimera
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …

    Promoter

    EF1A

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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