159,258 results

1. WDV-Echistatin

   Plasmid

   #226985

   Purpose

   Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

   Depositor

   Wendy Gordon

   Article

   Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

   Inserts

   WDV (repA )
   Echistatin
   

   Use

   Tags

   6xHis and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQE81XN-msYFP

   Plasmid

   #218960

   Purpose

   Expresses Hisx6-Tagged msYFP in bacteria

   Depositor

   Benjamin Glick

   Insert

   msYFP
   

   Use

   Tags

   6X-His

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PRS_8-GCaMP8m

   Plasmid

   #210400

   Purpose

   AAV-vector for fluorescent calcium measurements in the green spectrum from noradrenergic neurons

   Depositor

   Simon Wiegert

   Article

   Wissing et al bioRxiv 2022.01.22.477348

   Insert

   GCaMP8m
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PRSx8

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSpCas9(BB)-2A-Puro-C9orf72

   Plasmid

   #83439

   Purpose

   Expresses Cas9 and a gRNA targeting C9orf72

   Depositor

   Shawn Ferguson

   Article

   Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

   Insert

   gRNA targeting human C9orf72 (C9orf72 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPRKCZ-C1-EGFP (C-PRKCZ-C1)

   Plasmid

   #217759

   Purpose

   Fluorescent reporter for ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Protein kinase C zeta (PRKCZ Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C1 domain (aa 123-193)

   Promoter

   CMV

   Available since

   June 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. CXCR1-DuET

   Plasmid

   #213218

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CXCR1 (CXCR1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CCR6-DuET

   Plasmid

   #213202

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CCR6 (CCR6 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CCR3-DuET

   Plasmid

   #213199

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CCR3 (CCR3 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CCR4-DuET

   Plasmid

   #213200

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CCR4 (CCR4 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKD013

    Plasmid

    #207931

    Purpose

    Premade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M, for braiding

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

    Plasmid

    #174899

    Purpose

    Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PJEBAN3

    Plasmid

    #203682

    Purpose

    Expresses Yellow Fluorescent Protein (YFP) in Listeria monocytogenes

    Depositor

    Tine Rask Licht

    Article

    Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

    Insert

    Yellow Fluorescent Protein (YFP)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. iCAP-BI-48-KanR

    Plasmid

    #203533

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    AAV-BI48 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. iCAP-BI-62-KanR

    Plasmid

    #203535

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    AAV-BI62 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCh-alpha-tubulin

    Plasmid

    #49149

    Purpose

    expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

    Depositor

    Gia Voeltz

    Article

    Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

    Insert

    alpha tubulin (TUBA1B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-EFS-LSL-nlsGFP-shRNA

    Plasmid

    #175584

    Purpose

    Conditional express shRNA with control of LoxP

    Depositor

    Christian Wolfrum

    Article

    Sun et al Nature. 2020 Nov;587(7832):98-102. doi:

    Type

    Empty backbone

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pINDUCER20_mGFP

    Plasmid

    #190027

    Purpose

    Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal mEGFP

    Depositor

    Andrew Tutt

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB-U6insert-EF1puro

    Plasmid

    #104537

    Purpose

    A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

    Depositor

    George Church

    Article

    Kalhor et al Science. 2018 Aug 9. pii: science.aat980

    Type

    Empty backbone

    Use

    CRISPR, Mouse Targeting, and Synthetic Biology ; …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSBFP2-C1

    Plasmid

    #22880

    Depositor

    Dorus Gadella

    Article

    Kremers et al Biochemistry. 2007 Mar 27. 46(12):3775-8

    Insert

    SBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLY098-RetroEFS-BCMABBz-WPRE

    Plasmid

    #192199

    Purpose

    Retro-BCMACAR

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Retro-BCMACAR (TNFRSF17 Synthetic)
    

    Use

    Retroviral; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSCM001

    Plasmid

    #124605

    Purpose

    Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH

    Depositor

    David Summers

    Article

    Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1

    Insert

    mCherry-pHluorin
    

    Use

    Tags

    mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…

    Expression

    Bacterial

    Mutation

    Promoter

    pDAB

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CMV-mito-GEM-GECO1

    Plasmid

    #32461

    Purpose

    Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEM-GECO
    

    Use

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

    Promoter

    CMV

    Available since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1 His-NLRP1 codon-optimized

    Plasmid

    #166833

    Purpose

    Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tags

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-HA-H-Ras_wt

    Plasmid

    #39503

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS (HRAS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3/Myc-DNMT3A

    Plasmid

    #35521

    Purpose

    Mammalian expression of human DNMT3A with myc tag

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3A (DNMT3A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-nEFCas9

    Plasmid

    #87115

    Purpose

    SpCas9 expression AAV backbone plasmid under control of nEF promoter

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    nEF-Cas9
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDuRCC-K

    Plasmid

    #81193

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below., Please view…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO-shp38

    Plasmid

    #52920

    Purpose

    expresses a hairpin specific for human p38MAPK

    Depositor

    Sheila Stewart

    Article

    Alspach et al Cancer Discov. 2014 Mar 26.

    Insert

    shRNA targeting mitogen-activated protein kinase p38 (MAPK14 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-Duet1-His-TEV-mCherry-p62

    Plasmid

    #199780

    Purpose

    Used for the expression of mCherry labelled p62 (SMC Internal No. 391).

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    p62 (NUP62 Human)
    

    Use

    Tags

    6x His, TEV, mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EF.PGK.GFP

    Plasmid

    #17618

    Depositor

    Linzhao Cheng

    Article

    Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDIRE

    Plasmid

    #26745

    Depositor

    Rolf Zeller

    Article

    Osterwalder et al Nat Methods. 2010 Oct 17

    Insert

    iCre expression cassette
    

    Use

    Cre/Lox; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

    Plasmid

    #122151

    Purpose

    Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    dCas9 fused to SunTag(24x)
    

    Use

    Lentiviral

    Tags

    2xNLS-P2A-BFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti6.2_mCherry_roGFP2_Orp1

    Plasmid

    #155043

    Purpose

    Fluorescent redox reporter

    Depositor

    Vanessa LaPointe

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. HA-EGFP-HaloTag2

    Plasmid

    #41742

    Depositor

    Craig Crews

    Article

    Neklesa et al Nat Chem Biol. 2011 Jul 3;7(8):538-43. d

    Insert

    Halotag 2
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. NLS-mCherry

    Plasmid

    #221216

    Purpose

    control plasmid-part B of SPARK-OFF

    Depositor

    Xiaokun Shu

    Article

    Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

    Insert

    NLS-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pS648∙CsR

    Plasmid

    #197860

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Gm resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PEM7

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHelp

    Plasmid

    #227583

    Purpose

    Accessory plasmid with a sgRNA cassette for Cas9, and ampicillin resistance

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Insert

    guide RNA scaffold
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCI NL4-3 Nef-HA-WT

    Plasmid

    #24162

    Depositor

    Warner Greene

    Article

    Geleziunas et al Nature. 2001 Apr 12. 410(6830):834-8.

    Insert

    Nef (nef HIV)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

39. LCV2 STING KO

    Plasmid

    #217445

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human STING

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    sgRNA targeting STING (STING1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. 6xAP-1_RE::Renilla

    Plasmid

    #178317

    Purpose

    AP-1 Renilla luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    AP-1 Renilla Reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge