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163,606 results

Showing: 11751 - 11800 of 163606 results

  5. pFVK5

    Plasmid
    #167522
    Purpose
    Broad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, mKelly2, Gentamicin Resistance Marker.
    Depositor
    Christopher Reisch
    Article
    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629
    Insert
    VanRAM-mKelly2
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PVanCC
    Available Since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167522-plasmid-map-sequence-id-332934

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  6. TFORF0091

    Plasmid
    #142457
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZEB1 (ZEB1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142457-plasmid-map-sequence-id-397236

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  7. C3AR1-Tango

    Plasmid
    #66231
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    C3AR1 (C3AR1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. LiOn-CAG∞GFP

    Plasmid
    #154015
    Purpose
    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein EGFP from a CAG promoter upon action of the piggyBac transposase
    Depositor
    Jean Livet
    Article
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    Nov. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    154015-plasmid-map-sequence-id-317737

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  13. p663-UBC-miniTurbo-V5-CAMK1D_IDG-K

    Plasmid
    #158167
    Purpose
    Gateway destination clone of CAMK1D (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CAMK1D (CAMK1D Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available Since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    158167-plasmid-map-sequence-id-328029

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  16. TRUPATH Triple Ga15

    Plasmid
    #196060
    Purpose
    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196060-plasmid-map-sequence-id-385870

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  30. pMRX-IBU-pSu9-HaloTag7-SNAP

    Plasmid
    #217319
    Purpose
    Expresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.
    Depositor
    Noboru Mizushima
    Article
    Yang et al EMBO J. 2024 Nov;43(22):5613-5634. doi:
    Insert
    Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
    Use
    Retroviral
    Tags
    HaloTag and SNAP
    Expression
    Mutation
    Promoter
    CMV
    Available Since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    217319-plasmid-map-sequence-id-436845

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  36. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-WT

    Plasmid
    #218834
    Purpose
    Expresses N-terminal BirA*-tagged human wild type TLK1 variant 5 for BioID experiments.
    Depositor
    Travis Stracker
    Insert
    TLK1 (TLK1 Human)
    Use
    Tags
    FLAG-BirA*
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218834-plasmid-map-sequence-id-434423

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  37. pCSIIbleo-iRFP713(V256C)-P2A-EGFP

    Plasmid
    #198061
    Purpose
    The lentiviral vector for iRFP713(V256C) and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    iRFP713(V256C)
    Use
    Lentiviral
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    V256C mutation
    Promoter
    CAG
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198061-plasmid-map-sequence-id-389836

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  47. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid
    #112139
    Purpose
    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.
    Depositor
    Erik Sontheimer
    Article
    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:
    Inserts
    sgRNA scaffold
    Human-codon-optimized NmeCas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U1a and U6
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112139-plasmid-map-sequence-id-217823

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  50. pMega-MaPylRS

    Plasmid
    #200225
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    none
    Promoter
    proK-lacO and tacI
    Available Since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    200225-plasmid-map-sequence-id-400630

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Showing: 11751 - 11800 of 163606 results