161,488 results

1. pENTR5'_ubi:loxP-EGFP-loxP

   Plasmid

   #27322

   Depositor

   Leonard Zon

   Article

   Mosimann et al Development. 2011 Jan . 138(1):169-77.

   Insert

   ubiquitin:loxP-EGFP-loxP
   

   Use

   Multisite gateway 5' entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-Tag3B-CCre

   Plasmid

   #106369

   Purpose

   C-terminal part of the split-Cre System

   Depositor

   Johannes Hirrlinger

   Article

   Hirrlinger et al PLoS One. 2009;4(1):e4286. doi: 10.1371/

   Insert

   CCre
   

   Use

   Cre/Lox

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA flag PPAR gamma

   Plasmid

   #8895

   Purpose

   Mammalian expression of flag-tagged PPAR gamma

   Depositor

   Bruce Spiegelman

   Article

   Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

   Insert

   PPAR gamma (Pparg Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHAGE-FLT4

   Plasmid

   #116745

   Purpose

   Lentiviral expression of FLT4

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   FLT4 (FLT4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pT3-Neo-EF1a-GFP

   Plasmid

   #69134

   Purpose

   Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

   Depositor

   Martin Bonamino

   Article

   Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

   Type

   Empty backbone

   Use

   Sleeping beauty transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U3MSCV

   Available since

   Jan. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. hMLKL NB(1-182)-2xFv-Venus

   Plasmid

   #106077

   Purpose

   expression in mammalian cells

   Depositor

   Douglas Green

   Article

   Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

   Insert

   human MLKL NB(1-182)-2xFv-Venus (MLKL Human)
   

   Use

   Retroviral; Tet-on retrovirus

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   amino acids 1-182

   Promoter

   Dox-inducible

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. hSYN-mito-dsRED2

   Plasmid

   #173069

   Purpose

   mitochondria targeted dsRED2 expressed off of human Synapsin I promoter.

   Depositor

   Thomas Schwarz

   Insert

   Mito
   

   Use

   Lentiviral

   Tags

   dsRED

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin 1

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pC014 - LwCas13a-msfGFP

   Plasmid

   #91902

   Purpose

   Expresses active LwCas13a for mammalian RNA targeting

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a
   

   Use

   CRISPR

   Tags

   NLS and msfGFP-NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSEED-sfGFP

   Plasmid

   #219639

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   sfGFP SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PrimeRoot.v2C-FLP

    Plasmid

    #201740

    Purpose

    For DNA insertion test in protoplast

    Depositor

    Caixia Gao

    Article

    Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

    Insert

    ePPE,FLP
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBUN421

    Plasmid

    #62204

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    TaU3 and Ubi

    Available since

    Feb. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLZ12Km2-P23R:TA:Xen5

    Plasmid

    #88902

    Purpose

    Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

    Depositor

    Thomas Proft

    Article

    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

    Insert

    toxin-antitoxin + LuxABCDE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

13. pDONRp5-p2 GFP-NShroom3-iLID

    Plasmid

    #170976

    Purpose

    N-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.

    Depositor

    Miki Ebisuya

    Article

    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

    Insert

    NShroom3 (Shroom3 Mouse)
    

    Use

    Gateway cloning

    Tags

    eGFP and iLID

    Expression

    Mutation

    From isoform 2, deleted amino acids 1389 - 1805,…

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid

    #170977

    Purpose

    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

    Depositor

    Miki Ebisuya

    Article

    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

    Insert

    CShroom3 (Shroom3 Mouse)
    

    Use

    Gateway cloning

    Tags

    SspB and mCherry

    Expression

    Mutation

    Isoform X2, deleted aminoacids 1 - 1388

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNR230

    Plasmid

    #79546

    Purpose

    BxbI attB-attP. GFP will express if sites recombine.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Insert

    GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGBSedit Sham

    Plasmid

    #223200

    Purpose

    Group B Streptococcus Efficient Genome Editing with Modular crRNA

    Depositor

    Thomas Hooven

    Insert

    N/A
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGBScrispri Sham

    Plasmid

    #223201

    Purpose

    Group B Streptococcus inducible dCas12 CRISPRi with Modular crRNA

    Depositor

    Thomas Hooven

    Insert

    N/A
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-Frb-EGFP-HOTag6

    Plasmid

    #106919

    Purpose

    Express Frb-EGFP-HOTag6 fusion in mammalian cells.

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    Frb-EGFP-HOTag6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLG166

    Plasmid

    #124366

    Purpose

    Gfpmut2 constitutively expressed using the Ppen promoter for expression in B. subtilis

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    gfpmut2 (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppen

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMLS7-egfp

    Plasmid

    #112917

    Purpose

    Fluorescence microscopy

    Depositor

    Miguel Valvano

    Article

    Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PS7 promoter and Ps7

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSBinit

    Plasmid

    #110100

    Purpose

    E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTag

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Type

    Empty backbone

    Use

    Tags

    myc, His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPP089

    Plasmid

    #158796

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-GAL4

    Plasmid

    #24345

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    GAL4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pISH-beta2-microglobulin

    Plasmid

    #15883

    Depositor

    Peter Mombaerts

    Article

    Ishii et al Curr Biol. 2003 Mar 4. 13(5):394-400.

    Insert

    beta2-microglobulin (B2m Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pUASTattB-GFP-Msp300KASH

    Plasmid

    #170806

    Purpose

    Expresses Msp300 KASH domain fused to GFP under Gal4/UAS control for Drosophila transgenesis

    Depositor

    Vikki Weake

    Article

    Jauregui-Lozano et al Genetics. 2021 May 22. pii: 6281219. doi

    Insert

    GFP-Msp300KASH
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    5xUAS + hsp70 minimal promoter

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRN3P-HA-Suv4-20h1mut

    Plasmid

    #86690

    Purpose

    For transcription of of Suv4-20h1mut mRNA preceded by an HA-tag in 5'

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

    Insert

    histone-lysine N-methyltransferase KMT5B mutant (Kmt5b Mouse)
    

    Use

    Tags

    HA

    Expression

    Mutation

    mutated region NHDC (asparagine 273 to cysteine 2…

    Promoter

    T3

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJW1821

    Plasmid

    #163092

    Purpose

    CRISPR repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    mScarlet^SEC (Lox511I)^::3xMyc
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p323-L10A-PATagRFP

    Plasmid

    #74172

    Purpose

    Fluorescent reporter for single ribosome tracking

    Depositor

    Robert Singer

    Article

    Katz et al Elife. 2016 Jan 13;5. pii: e10415. doi:

    Insert

    ribosomal protein L10A (Rpl10a Rat)
    

    Use

    Lentiviral

    Tags

    PATagRFP

    Expression

    Mutation

    Promoter

    UbC

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAcCas12nHS_ABE_D3_empty

    Plasmid

    #203809

    Purpose

    Plasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    ABE_D3
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-CAG-HcKCR1-EYFP

    Plasmid

    #182021

    Purpose

    Express light-gated potassium channel HcKCR1-EYFP in mammalian cells

    Depositor

    Mingshan Xue

    Article

    Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

    Insert

    HcKCR1
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pNR276

    Plasmid

    #79577

    Purpose

    TP901 attB-attP. GFP will express if sites recombine.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Insert

    GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pNR239

    Plasmid

    #79576

    Purpose

    A118 attB-attP. GFP will express if sites recombine.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Insert

    GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Lamp1-mCherry-FRB

    Plasmid

    #186576

    Purpose

    Expression of mCherry-FRB at the outer surface of the lysosome using LAMP1

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Tags

    mCherry-FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBS185 CMV-Cre

    Plasmid

    #11916

    Purpose

    Mammalian expression of Cre recombinase driven by the human cytomegalovirus IE promoter

    Depositor

    Brian Sauer

    Article

    Sauer et al New Biol. 1990 May . 2(5):441-9.

    Insert

    cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. TfR-pHuji

    Plasmid

    #61505

    Purpose

    Human transferrin receptor tagged with pH sensitive red fluorescent protein pHuji

    Depositor

    David Perrais

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    Transferrin receptor (TFRC Human)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLenti-TagGFP2

    Plasmid

    #121426

    Purpose

    TAgGFP2 was amplified from pLenti-Origene-Nrf21 and inserted into XhoI and EcoRI digested pLenti-Origene-Nrf21.

    Depositor

    Ovijit Chaudhuri, Stanley Qi

    Article

    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

    Insert

    empty TagGFP2
    

    Use

    Lentiviral

    Tags

    TagGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid

    #124536

    Purpose

    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Inserts

    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. RRM1 E2.3 gRNA

    Plasmid

    #90881

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RRM1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RRM1 (Guide Designation E2.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCSIIbsr-Eevee-ROCK-NES

    Plasmid

    #209918

    Purpose

    A FRET biosensor for ROCK activity

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    Eevee-ROCK-NES
    

    Use

    Lentiviral

    Tags

    ECFP and YPet

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA-FLAG-Hu-Trip6

    Plasmid

    #27255

    Depositor

    Thomas Gilmore

    Article

    Zhao et al Gene Expr. 1999 . 8(4):207-17.

    Insert

    Trip6 (TRIP6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

41. pCAGGS 3xFLAG Mbnl2

    Plasmid

    #217032

    Purpose

    Overexpression of Mbnl2 in mammalian cells

    Depositor

    Chaolin Zhang

    Article

    Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

    Insert

    Mbnl2 (Mbnl2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pOD_009

    Plasmid

    #159520

    Purpose

    Rifampicin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

    Depositor

    Elisabeth Raleigh

    Article

    Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. pFsynW KDEL Hook

    Plasmid

    #197277

    Purpose

    Encodes ER-targeted streptavidin-KDEL hook (RUSH system).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    Streptavidin
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pKM7

    Plasmid

    #154164

    Purpose

    mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pMC-6-Ruby-9

    Plasmid

    #197740

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    Ruby reporter tricistronic CDS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR

    Plasmid

    #210523

    Purpose

    AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    GFP-KASH
    U6-gRNA-directcapture
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pHDR_DDX4-T2A-tdTomato-puroTK

    Plasmid

    #222519

    Purpose

    Homology directed repair template for knocking in tdTomato reporter to DDX4.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    tdTomato
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pDAC627

    Plasmid

    #195240

    Purpose

    Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. H2B-GFP-Aurora B WT

    Plasmid

    #184042

    Purpose

    Expresses H2B-GFP fused to Aurora B WT

    Depositor

    Lienhard Schmitz

    Article

    Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

    Insert

    AURKB (AURKB Human)
    

    Use

    Tags

    EGFP and H2B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pBUN250

    Plasmid

    #60672

    Purpose

    overexpression of M. smegmatis ddl gene in Mycobacterium

    Depositor

    Raul Barletta

    Article

    Feng et al Antimicrob Agents Chemother. 2003 Jan;47

    Insert

    ddl
    

    Use

    E.coli-mycobacterium shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    BCG Hsp60

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only