159,333 results

1. pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

   Plasmid

   #85006

   Purpose

   For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   EGFP-ires-tTA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHR-SFFV-dCas9-BFP

   Plasmid

   #46910

   Purpose

   Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP

   Depositor

   Jonathan Weissman, Stanley Qi

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-BFP fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2xNLS, BFP, and HA

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pR-mKate2

   Plasmid

   #228610

   Purpose

   Mammalian expression of mKate2, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

   Insert

   mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC19-hAPOE-R-loop

   Plasmid

   #134901

   Purpose

   Generation and purification of R-loops for in vitro biochemistry

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Insert

   APOE promoter (APOE Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC19-hSNRPN-R-loop

   Plasmid

   #134902

   Purpose

   Generation and purification of R-loops for in vitro biochemistry

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Insert

   hSNRPN promoter (SNRPN Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. KRphi-mRFP

   Plasmid

   #17276

   Depositor

   Sergio Grinstein

   Article

   Yeung et al Science. 2006 Jul 21. 313(5785):347-51.

   Insert

   KR phi
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pGST2-GST-TEV-OPTN

   Plasmid

   #198428

   Purpose

   Used for the expression and purification of OPTN (WT) (SMC Internal No. 1587)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   optineurin (OPTN Human)
   

   Use

   Tags

   GST and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEF6-Acly

   Plasmid

   #70765

   Purpose

   Expresses mouse ATP-citrate lyase in mammalian cells

   Depositor

   Kathryn Wellen

   Article

   Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

   Insert

   ATP Citrate Lyase (Acly Mouse)
   

   Use

   Tags

   myc-epitope

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBlueKan+cysMPro

   Plasmid

   #187879

   Purpose

   Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.

   Depositor

   Gaylen Uhlich

   Article

   Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CysM

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

    Plasmid

    #92390

    Purpose

    expresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cells

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHMR1A

    Plasmid

    #102957

    Purpose

    gene cloning of MR1A

    Depositor

    Gennaro De Libero, Lucia Mori

    Article

    Lepore et al Nat Commun. 2014 May 15;5:3866. doi: 10.

    Insert

    MR1A cDNA (MR1 Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Stop codon has been removed by HindIII site

    Promoter

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDual_dsCas9_Blast

    Plasmid

    #214681

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites

    Depositor

    Kevin Janes

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMaCTag-P13

    Plasmid

    #120024

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry
    

    Use

    Pcr template

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcKv10.1

    Plasmid

    #85703

    Purpose

    mammalian expression Kv10.1

    Depositor

    Luis Pardo

    Article

    Chen et al PLoS One. 2011 May 3;6(5):e19257. doi: 1

    Insert

    KCNH1 (KCNH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. UKNP2.4.1

    Plasmid

    #98212

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-GFP11x7-HygR

    Plasmid

    #223676

    Purpose

    To check the split GFP signals in mitochondria (2)

    Depositor

    Juan Melero-Martin

    Article

    Lin et al Nature. 2024 May;629(8012):660-668. doi:

    Insert

    GFP11x7
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. ERM-APEX2

    Plasmid

    #79055

    Purpose

    Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    APEX2 anchored to the cytosolic face of the ER membrane
    

    Use

    Lentiviral

    Tags

    Residues 1-27 of P450 oxidase 2C1 and V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV immearly promotor

    Available since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. qTAG-N-Puro-dTAG

    Plasmid

    #207694

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. phOCT4-Luc

    Plasmid

    #17221

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

    Insert

    Upstream region of human OCT4 (POU5F1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMC-1-StUbi-6

    Plasmid

    #197710

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    StUbi promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pC1-HyPerRed-mito

    Plasmid

    #60247

    Purpose

    Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HyPerRed-mito
    

    Use

    Tags

    Mitochondrial targeting signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mIFP-N1

    Plasmid

    #54620

    Purpose

    Localization: N1 Cloning Vector, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson, Xiaokun Shu

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Type

    Empty backbone

    Use

    Tags

    mIFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pBAMD1-6

    Plasmid

    #61566

    Purpose

    Standardized mini-Tn5 delivery plasmid, gentamicin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9

    Plasmid

    #175523

    Purpose

    Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    LAMP1-HaloTag11 and Tomm20-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

    Plasmid

    #216156

    Purpose

    Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mScarlet with C-terminal FLAG tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Flamindo2

    Plasmid

    #73938

    Purpose

    Expresses biosensor for cAMP in mammalian cells

    Depositor

    Tetsuya Kitaguchi

    Article

    Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

    Insert

    Flamindo2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LeGO-hEµMAR-G2

    Plasmid

    #52633

    Purpose

    3rd generation lentiviral vector for enhanced eGFP expression/marking in primary B cells and B-cell lines

    Depositor

    Boris Fehse

    Article

    Mock et al Hum Gene Ther Methods. 2012 Dec;23(6):40

    Insert

    hEµMAR-SFFV; eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Human LFA-1 alpha

    Plasmid

    #8630

    Depositor

    Timothy Springer

    Article

    Larson et al J Cell Biol 1989 Feb;108(2):703-12.

    Insert

    LFA1 alpha (ITGAL Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

29. pDONRPEX18Tp-SceI-pheS

    Plasmid

    #111608

    Purpose

    Gateway compatible allelic exchange vector for Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Type

    Empty backbone

    Use

    Allelic exchange in burkholderia sp.

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Flag-FOXM1

    Plasmid

    #153136

    Purpose

    Expression of Flag tagged human FOXM1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXM1 (FOXM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLenti CMV/TO GFP-Zeo DEST (719-1)

    Plasmid

    #17431

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-Zeo

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. mu-GM-CSF-pCIneo

    Plasmid

    #163518

    Purpose

    Expresses murine GM-CSF in mammalian cells

    Depositor

    Thomas Weber

    Insert

    murine GM-CSF
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA

    Plasmid

    #160141

    Purpose

    Cre- dependent expression of Sun1GFP for nuclei isolation

    Depositor

    Andreas Pfenning

    Article

    Lawler et al J Neurosci. 2020 Nov 10. pii: JNEUROSCI.

    Insert

    Sun1-GFP (Sun1 Mouse)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N-truncated: removed amino acids 1-207, retained …

    Promoter

    Ef1a

    Available since

    March 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p16sheLL

    Plasmid

    #37320

    Depositor

    John Schiller

    Article

    Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

    Insert

    HPV16L1+L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA

    Plasmid

    #37090

    Purpose

    Expresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    hChr2(H134R)-mCherry
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGEX SH3(2)-4R

    Plasmid

    #112090

    Purpose

    Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3(2)-4R (NCK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. tdTomato-pBAD

    Plasmid

    #54856

    Purpose

    Localization: Protein Expression Vector , Excitation: 554, Emission: 581

    Depositor

    Michael Davidson, Roger Tsien, Nathan Shaner

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Type

    Empty backbone

    Use

    Tags

    6xHis and tdTomato

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. eGFP-ova-C1

    Plasmid

    #163524

    Purpose

    Expresses both eGFP and chicken ovalbumin in mammalian cells

    Depositor

    Thomas Weber

    Insert

    enhanced-GFP-chicken ovalbumin tandem
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRAM29

    Plasmid

    #234315

    Purpose

    G264A Cat-RNA U1367, Catalytically dead ribozyme control

    Depositor

    James Chappell

    Article

    Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

    Insert

    Splicing-deficient mutant of ribozyme
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    P. CymRC

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDONR221_SLC2A2

    Plasmid

    #132126

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC2A2 (SLC2A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge