179,521 results
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xCas9(3.7)-BE3
Plasmid#108380PurposeMammalian expression vector for xCas9(3.7)-BE3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertxCas9(3.7)-BE3
TagsNLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mTagBFP2-Lifeact-7
Plasmid#54602PurposeLocalization: Actin, Excitation: 399, Emission: 456DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLifeact-mTagBFP2
ExpressionMammalianPromoterCMVAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
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PKC alpha WT
Plasmid#21232DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPKC alpha (PRKCA Human)
TagsHAExpressionMammalianPromoterCMVAvailable sinceAug. 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PL-SIN-EOS-C(3+)-EiP
Plasmid#21313PurposeLentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistanceDepositorPublicationInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralExpressionMammalianPromoterEOS-C(3+) promoter regionAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDest-565
Plasmid#11520PurposeGateway destination vector for bacterial expression; His,GST tagDepositorTypeEmpty backboneUseGateway CloningTags6xHis and GSTExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV5B-TGFbeta receptor II wt
Plasmid#11766DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTGFbeta receptor II (TGFBR2 Human)
TagsHAExpressionMammalianAvailable sinceMay 12, 2006AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
dCas9-VPR_P2A_mCherry
Plasmid#154193PurposeLentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry markerDepositorInsertsp-dCas9-VPR_P2A_mCherry
UseLentiviralExpressionMammalianMutationD10A, H840APromoterEF1asAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCM-AAVS1-TO-hNGN2
Plasmid#105840PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuronDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseCRISPR and TALENPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pscAAV-CAG-GFP
Plasmid#83279Purposerecombinant AAV vector packaging self-complementary GFP under the CAG promoterDepositorInsertenhanced green fluorescent protein
UseAAVExpressionMammalianPromoterCAGAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH-EF1-Luc2-P2A-tdTomato
Plasmid#72486PurposeExpress Luc2 and tdTomato under EF1 promoterDepositorInsertLuc2-P2A-tdTomato
UseLentiviralExpressionMammalianPromoterEF1Available sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-sgRosa26-1_CBh-Cas9-T2A-BFP
Plasmid#64216PurposeExpression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptideDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
sgRNA targeting ROSA26-1
UseCRISPRTags3xFLAG, NLS, and T2A-BFPExpressionMammalianPromoterCBh and U6Available sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pdCas9
Plasmid#46569PurposeExpresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.DepositorPublicationInsertstracrRNA
dcas9
CRISPR array
UseCRISPRExpressionBacterialMutationD10A, H840AAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-GEM-GECO1
Plasmid#32442PurposeMammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGEM-GECO1
ExpressionMammalianMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
deltaNp63alpha-FLAG
Plasmid#26979DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertΔNP63alpha (TP63 Human)
TagsFLAGExpressionMammalianMutationdominant negative isoform of p63; aa 1–587Available sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
4xM67 pTATA TK-Luc
Plasmid#8688DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert4 repeated M67 sites
UseLuciferaseExpressionMammalianAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBABE-bleo IGF-1R
Plasmid#11212DepositorAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBABE-hygro p53 DD
Plasmid#9058DepositorPublicationInsertp53 DD (Trp53 Mouse)
UseRetroviralExpressionMammalianMutationDD. Dominant negative mutant carries deletion of…Available sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSFFV_mNG2(11)1-10
Plasmid#82610PurposeLentiviral vector of constitutive expression of mNeonGreen2(1-10)DepositorInsertmNeonGreen2(1-10)
UseLentiviralExpressionMammalianMutationK128M, S142T, R150M, G172V and K213MPromoterSFFVAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Ef1a-DIO eNpHR 3.0-EYFP
Plasmid#26966PurposeAAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibitionDepositorPublicationHas serviceAAV1, AAV5, AAV9, and AAV RetrogradeInserteNpHR 3.0
UseAAVTagsEYFPExpressionMammalianMutationER export at 3' end of gene. Trafficking sig…PromoterEF1alphaAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-myc3-CUL1
Plasmid#19896DepositorPublicationAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hBax C3-EGFP
Plasmid#19741DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBax (BAX Human)
TagsGFPExpressionMammalianMutationnoneAvailable sinceNov. 10, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBabe-Puro-IKBalpha-mut (super repressor)
Plasmid#15291DepositorPublicationInsertinhibitor of Kappa B alpha (NFKBIA Human)
UseRetroviralExpressionMammalianMutationS32A/S36A: “super-repressor” allele (resistant to…Available sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-rab11 DN
Plasmid#12678DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRAB11A (Homo sapiens) (RAB11A Human)
TagsGFP and HAExpressionMammalianMutationchanged serine 25 to asparagineAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro
Plasmid#141391PurposeLentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-N (N SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pX330A-FBL/PITCh
Plasmid#63671PurposeExpresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-TO-dCas9-3XmCherry
Plasmid#64108PurposeExpression of Sp dCas9-3XmCherry in mammalian cellsDepositorInsertSp dCas9
UseCRISPR and LentiviralTags3XmCherryExpressionMammalianPromoterCMV-TOAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLIK-Hygro
Plasmid#25737Purpose3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.DepositorTypeEmpty backboneUseLentiviral and RNAiExpressionMammalianAvailable sinceAug. 13, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR221_EGFP
Plasmid#25899PurposeGateway entry vector containing EGFP.DepositorInsertEGFP
UseGateway CloningAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA-HIF1alpha-wt-pBabe-puro
Plasmid#19365DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationwt human cDNAAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-C2-NLRP3
Plasmid#73955PurposeExpress EGFP-tagged human NLRP3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
TagsEGFPExpressionMammalianPromoterCMV iEAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQCXIH-Myc-YAP-5SA
Plasmid#33093DepositorInsertYAP (YAP1 Human)
UseRetroviralTagsMycExpressionMammalianMutationS61A, S109A, S127A, S164A, S381A (phosphorylation…PromoterCMV/MSVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP ZO1
Plasmid#30313DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertZonula Occludens-1 (TJP1 Human)
TagsGFPExpressionMammalianMutationUnique spliceform*Available sinceAug. 8, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR5'_ubi
Plasmid#27320PurposeGateway entry vector containing 3.5 kb ubi promoter fragment, with L4 and R1 sequences for MultiSite Gateway cloningDepositorInsertzebrafish ubiquitin promoter (ubb Zebrafish)
UseMultisite gateway 5' entry vectorAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUltra-hot
Plasmid#24130Purpose3rd generation Lentiviral vector for bi-cistronic expression of mCherry and the gene of interestDepositorTypeEmpty backboneUseLentiviralTagsmCherryExpressionMammalianAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pIRESneo-FLAG/HA Ago1
Plasmid#10820DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertArgonaute 1 (AGO1 Human)
TagsFLAG and HAExpressionMammalianAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCmini-iCAP-AAV.CAP-B10
Plasmid#175004Purposenon-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation systemDepositorInsertSynthetic construct isolate AAV.CAP-B10 VP1 gene
UseAAVExpressionMammalianMutation7 amino acid substitution between VP1 452 and VP1…Promoterp41Available sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lenti-sgRNA blast
Plasmid#104993PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by
