166,041 results

1. pAAV-hSyn-GRAB_VIP1.0

   Plasmid

   #208681

   Purpose

   Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTn7-SCOUT11

   Plasmid

   #200991

   Purpose

   pLv1-pUC18R6KT-miniTn7-Gm, GmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. hHSF1 RNAi resistant

   Plasmid

   #118347

   Purpose

   This plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).

   Depositor

   Lea Sistonen

   Article

   Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

   Insert

   hHSF1 (HSF1 Human)
   

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   RNAi resistance mutations (does not change amino …

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MT-AvrXa10-FT

   Plasmid

   #234373

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus

   Depositor

   Michael Smanski

   Article

   Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202

   Inserts

   NbGP41-GFP-AvrXa10-GB1
   dCas9-24XGP41
   gRNAs targeting FT locus
   RFP
   

   Use

   Synthetic Biology

   Tags

   GB1, GFP, and GP41

   Expression

   Plant

   Promoter

   Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. opto-Itsn1

   Plasmid

   #174509

   Purpose

   Expresses opto-Itsn1 (Itsn1DH-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   opto-Itsn1-mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. (SUMO)10-(SIM)5

   Plasmid

   #126866

   Purpose

   Bacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
   

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Plasmid

   #176534

   Purpose

   Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associated IscB (Delaware Bay metagenome)
   

   Tags

   14xHis-MBP-bdSUMO and TwinStrep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSII406

   Plasmid

   #35442

   Depositor

   Steven Haase

   Article

   Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

   Type

   Empty backbone

   Use

   Yeast integrating vector

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. BcLOV4 fusion tools screening plasmid #1

   Plasmid

   #174511

   Purpose

   Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)Cy-

    Plasmid

    #191390

    Purpose

    Donor for targeted integration of hCdt1(1-100)Cy- probe to the hROSA26 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hCdt1(1-100)Cy-
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRX-INU-FLAG-FAM134Bs

    Plasmid

    #128261

    Purpose

    Expresses FAM134Bs in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    FAM134Bs (RETREG1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. opto-Tiam1

    Plasmid

    #174508

    Purpose

    Expresses opto-Tiam1 (BcLOV4-GGGSx2-Tiam1DH-GGGSx2-mCherry) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    opto-Tiam1-mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-Miro1v4

    Plasmid

    #174111

    Purpose

    Expresses the peroxisomal variant (splice variant 4) of Miro with an N-terminal EGFP.

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Miro1 (var4, peroxisomal splice variant)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGMβ1

    Plasmid

    #216202

    Purpose

    Chromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.

    Depositor

    Yasuko Sasaki

    Article

    Iwamoto et al Biosci Microbiota Food Health. 2022;41(1

    Type

    Empty backbone

    Use

    Shuttle vector, conjugal plasmid, theta type rep…

    Expression

    Bacterial

    Promoter

    lac promoter in pGEM-Teasy,　Promoters in pAMbeta1…

    Available Since

    Jan. 21, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pBad-HisB-GRvT

    Plasmid

    #87363

    Purpose

    Expresses GRvT, a tandem heterodimer green and red fluorescent protein

    Depositor

    Robert Campbell

    Article

    Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

    Insert

    GRvT
    

    Tags

    His-Tag

    Expression

    Bacterial

    Promoter

    AraBad

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-Lck-FRB-mTurquoise2

    Plasmid

    #176130

    Purpose

    Plasma membrane localized FRB, rapamycin heterodimerization

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    FRB
    

    Use

    Lentiviral

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3 Flag MKK7b1(glu)

    Plasmid

    #14673

    Depositor

    Roger Davis

    Article

    Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

    Insert

    MKK7b1(Glu) (Map2k7 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser-269Glu Thr-273Glu

    Available Since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

18. BcLOV4 fusion tools screening plasmid #2

    Plasmid

    #174512

    Purpose

    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
    

    Tags

    3xFLAG and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. SSpB-iRFP-p63DH

    Plasmid

    #176120

    Purpose

    Heterodimerization with iLID, RhoA activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    RHOGEF p63 (ARHGEF25 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFRT-DestFLAGHA_HuD

    Plasmid

    #65760

    Purpose

    constitutively expresses FLAG-HA-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pMSCV2.2-IRES- GFP-Opa1

    Plasmid

    #191971

    Purpose

    Plasmid for cloning reaction, containing OPA1 coding sequence.

    Depositor

    Luca Scorrano

    Insert

    GFP-Opa1
    

    Use

    Retroviral

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. Lenti-sgCdkn2a/Cre

    Plasmid

    #89644

    Purpose

    Expresses a Cdkn2a-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Cdkn2a (Cdkn2a Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. SspB-iRFP-DHPH-TIAM1

    Plasmid

    #176118

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJUMP18-Uac

    Plasmid

    #126959

    Purpose

    Universal Acceptor Plasmid. The fusion sites of the basic part generated are introduced during domestication. sfGFP reporter gene. High copy number oriV #8.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV_SEPT9_i3-msfGFP

    Plasmid

    #180322

    Purpose

    mammalian expression of human SEPT9_i3 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v3 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pBa.TfR-Halo

    Plasmid

    #162716

    Purpose

    Mammalian expression of human TFRC

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    TfR (TFRC Human) (TFRC Human)
    

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta actin

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pTn7-SCOUT13

    Plasmid

    #200993

    Purpose

    pLv1-pUC18R6KT-miniTn7-Tc, TcR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTn7-SCOUT12

    Plasmid

    #200992

    Purpose

    pLv1-pUC18R6KT-miniTn7-Km, KmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKM492-Myc-His

    Plasmid

    #109283

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with Myc-His tag

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    attB-Myc-His
    

    Use

    Orbit integrating plasmid

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-hSyn-GRAB_CCK1.0

    Plasmid

    #208673

    Purpose

    Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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31. rpoDs366C

    Plasmid

    #129688

    Purpose

    Expression of Sigma 70 S366C

    Depositor

    Jeff Gelles

    Article

    Mumm et al bioRxiv 2020

    Insert

    rpoD (rpoD E. coli)
    

    Tags

    His6/thrombin

    Expression

    Bacterial

    Mutation

    C132S C291S C295S S366C

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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32. CL20_mEGFP_UBXN4_R3HDM1

    Plasmid

    #205950

    Purpose

    Express mEGFP-tagged fusion protein, UBXN4_R3HDM1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    UBXN4_R3HDM1 (UBXN4, R3HDM1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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33. p3E-EEA1

    Plasmid

    #109560

    Purpose

    Multisite gateway vector for 5' tagging with early endosome associated 1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    early endosome associated 1 (eea1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHAGE pGK hNLRP3-mNG

    Plasmid

    #218643

    Purpose

    Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak pGK promoter with blast selection

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    pGK

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTR-177 pUC19-CLTA-N-mCherry

    Plasmid

    #186128

    Purpose

    knockin of mCherry to CLTA target

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CLTA (CLTA Human)
    

    Use

    CRISPR

    Mutation

    WT with mCherry sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. SRM

    Plasmid

    #25490

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SRM (SRM Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

37. pEBG-MKK4-S255D/T259E (GST)

    Plasmid

    #21566

    Depositor

    Leonard Zon

    Article

    Sanchez et al Nature. . 372(6508):794-8.

    Insert

    mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Serine 255 to Aspartic Acid, Threonine 259 to Gl…

    Available Since

    July 17, 2009

    Availability

    Academic Institutions and Nonprofits only

38. pUBQ10-YFP-GW

    Plasmid

    #209362

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter driving expression of N-terminal YFP fusion protein.

    Depositor

    Lucia Strader

    Article

    Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. VAPB_Halo_C_allele

    Plasmid

    #178174

    Purpose

    Donor vector for endogenous tagging of human VAPB at the C-terminus with halotag

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Halotag (VAPB Human)
    

    Use

    Donor vector

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLV-mCherry-FKBP-TIAM1(DHPH)

    Plasmid

    #176138

    Purpose

    Heterodimerization with FRB (rapamycin), Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge