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163,570 results

Showing: 1001 - 1040 of 163570 results

  1. 1a. pETM11-SUMO-SNCA-GFP

    Plasmid
    #107292
    Purpose
    Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility
    Depositor
    Dmytro Yushchenko
    Article
    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191
    Insert
    SNCA-GFP (SNCA Human)
    Use
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    107292-plasmid-map-sequence-id-209472

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  3. SRPK1 (SRPK1A-c100)

    Plasmid
    #98246
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SRPK1A (SRPK1 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    98246-plasmid-map-sequence-id-193287

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  8. pB1H2 UV5 Zif268-cons (2-hybrid bait plasmid)

    Plasmid
    #128164
    Purpose
    UV5 promoter drives expression of Zif268-consensus peptide fusion. Pair with pGHUC w-ERBIN as a positive control (turns on HIS3/GFP).
    Depositor
    Marcus Noyes
    Article
    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928
    Insert
    10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
    Use
    Synthetic Biology
    Tags
    FLAG and zif268
    Expression
    Bacterial
    Mutation
    Promoter
    UV5
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    128164-plasmid-map-sequence-id-267570

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  10. pGHUC w-ERBIN (2-hybrid prey plasmid)

    Plasmid
    #128163
    Purpose
    UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).
    Depositor
    Marcus Noyes
    Article
    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928
    Insert
    ERBIN PDZ domain (ERBIN Synthetic)
    Use
    Synthetic Biology
    Tags
    FLAG and Omega subunit of RNA polymerase
    Expression
    Bacterial
    Mutation
    Promoter
    UV5
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    128163-plasmid-map-sequence-id-267568

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  11. pDONR221-SPNS1_STOP

    Plasmid
    #161439
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SPNS1 (SPNS1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161439-plasmid-map-sequence-id-355837

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  13. pCAG-MCS-EGFP

    Plasmid
    #164092
    Purpose
    expression of EGFP-tagged proteins under CAG promoter
    Depositor
    Maik Hintze
    Insert
    EGFP
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    164092-plasmid-map-sequence-id-322571

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  16. pCAGG-NFIB2

    Plasmid
    #112700
    Purpose
    Expression of NFIB2 protein and GFP
    Depositor
    Richard Gronostajski
    Insert
    Nuclear Factor I B2 (Nfib Mouse)
    Use
    Tags
    GFP and HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112700-plasmid-map-sequence-id-212390

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  17. pAAV-TRE-DIO-ChR2-EYFP

    Plasmid
    #183765
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The ChR2-EYFP expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Yu et al Science. 2022 Jul;377(6601):63-72. doi:
    Insert
    AAV transgene -teto promoter-flex/dio-ChR2-EYFP
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    ChR2-EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    tetO promoter
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183765-plasmid-map-sequence-id-361682

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  19. CLYBL hOPM

    Plasmid
    #112499
    Purpose
    Homologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in CLYBL locus
    Depositor
    Bruce Conklin
    Insert
    OTX2, PAX6, MITF (PAX6, MITF, OTX2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    112499-plasmid-map-sequence-id-223597

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  21. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

    Plasmid
    #107581
    Purpose
    B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    mCherry
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pdh promoter Bacillus megaterium DSM319
    Available Since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    107581-plasmid-map-sequence-id-209155

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  24. pMito-MtrxRFP2

    Plasmid
    #172324
    Purpose
    fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)
    Depositor
    Huiwang Ai
    Article
    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.
    Insert
    mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
    Use
    Tags
    His-tag and Mitochondria localization sequence
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, SP6
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172324-plasmid-map-sequence-id-431104

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  25. pKSJ376

    Plasmid
    #103065
    Purpose
    Expresses colicin E1 with R domain deletion and ImmE1
    Depositor
    Karen Jakes, Alan Finkelstein
    Article
    Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0
    Inserts
    colicin E1 immunity protein (pColE1_26, imm E. coli)
    cea/colicin E1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    R (receptor-binding domain) deletion in colicin E…
    Promoter
    T7 lac (from pET) and T7 lac (from pET52-b)
    Available Since
    Dec. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    103065-plasmid-map-sequence-id-200637

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  28. pCMV-iGlu3Fast

    Plasmid
    #226500
    Purpose
    Ultrafast-decay iGluSnFR3 variant for tracking high frequency synaptic glutamate release
    Depositor
    Katalin Torok
    Article
    Tran et al bioRxiv, August 30th, 2024
    Insert
    iGluSnFR3 S72T
    Use
    Tags
    IgK, Myc tag, and PDGFR Beta TM domain
    Expression
    Mammalian
    Mutation
    S72T
    Promoter
    CMV
    Available Since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    226500-plasmid-map-sequence-id-448688

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  30. pDIV313

    Plasmid
    #204956
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus fumigatus U3 promoter, Aspergillus ni…
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204956-plasmid-map-sequence-id-415598

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  31. HPSE-bio-His

    Plasmid
    #53407
    Purpose
    Expresses full-length Heparanase precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    HPSE (HPSE Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53407-plasmid-map-sequence-id-83186

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  34. pHBintT

    Plasmid
    #48134
    Purpose
    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vector
    Depositor
    Dieter Jahn
    Article
    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    48134-plasmid-map-sequence-id-69938

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  35. BII-tdT-inv-GFP

    Plasmid
    #133393
    Purpose
    Piggybac vector for bidirectional promoter reporter assay
    Depositor
    Blair Madison
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    No promoter
    Available Since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133393-plasmid-map-sequence-id-268036

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Showing: 1001 - 1040 of 163570 results