8,789 results

1. pMV306G13+Lux

   Plasmid

   #26160

   Depositor

   Brian Robertson, Siouxsie Wiles

   Article

   Andreu et al PLoS One. 2010 May 24;5(5):e10777.

   Insert

   G13 promoter + Bacterial luciferase operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   It contains a gram-positive enhanced translation …

   Promoter

   Available Since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX307 P2A GFP Luciferase

   Plasmid

   #193674

   Purpose

   Constitutive lentiviral expression of Luciferase

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti_dCas9-KRAB-MeCP2

   Plasmid

   #122205

   Purpose

   Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   Lentiviral

   Tags

   KRAB-MeCP2

   Expression

   Mammalian

   Mutation

   Cas9 (D10A, H840A)

   Promoter

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-ASH1L

   Plasmid

   #65369

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   ASH1L (ASH1L Human)
   

   Use

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-Foxo1ADA (pCMV5)

   Plasmid

   #12143

   Depositor

   Domenico Accili

   Article

   Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

   Insert

   Foxo1 ADA (Foxo1 Mouse)
   

   Use

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Phosphorylation-deficient, also referred to as co…

   Promoter

   CMV

   Available Since

   Aug. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWPXL-Sox9

   Plasmid

   #36979

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Sox9 (Sox9 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2C-3XtbGFP-PEST

   Plasmid

   #69072

   Purpose

   Expresses 3XturboGFP-NLS-PEST under MERVL_LTR

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

   Insert

   turboGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLNCX2 ER:ras

   Plasmid

   #67844

   Purpose

   4-hydroxytamoxifen inducible ER:ras fusion protein

   Depositor

   Masashi Narita

   Article

   Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

   Insert

   ER1a H-RasG12V fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G525R renders the HBD largely insensitive to 17ß‐…

   Promoter

   CMV

   Available Since

   Aug. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGB3alpha2_35S:P19:Tnos (GB1203)

   Plasmid

   #68214

   Purpose

   Transcriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   P19 silencing suppressor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   35S

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. SBE-Luc

    Plasmid

    #16527

    Depositor

    Bert Vogelstein

    Article

    Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

    Insert

    Smad binding element
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

11. EF.PGK.GFP

    Plasmid

    #17618

    Depositor

    Linzhao Cheng

    Article

    Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_PGK

    Plasmid

    #79120

    Purpose

    Lentiviral vector for constitutive expression of genes (PGK promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 APEX2-NES

    Plasmid

    #49386

    Purpose

    Expresses APEX2 in the cytosol of mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Flag - APEX2 - NES
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available Since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV5-Smad7-HA

    Plasmid

    #11733

    Depositor

    Jeff Wrana

    Article

    Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

    Insert

    SMAD7 (SMAD7 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA3.1-3xFLAG-TEV-UBE2O

    Plasmid

    #105718

    Purpose

    Expresses WT full length human N- terminally tagged UBE2O

    Depositor

    Ramanujan Hegde

    Article

    Yanagitani et al Science. 2017 Aug 4;357(6350):472-475. d

    Insert

    UBE2O (UBE2O Human)
    

    Use

    Tags

    3xFLAG-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (from vector)

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-DsRed2

    Plasmid

    #15777

    Depositor

    Edward Callaway

    Article

    Wickersham et al Neuron. 2007 Mar 1. 53(5):639-47.

    Insert

    DsRed2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a-Streptavidin-Alive

    Plasmid

    #20860

    Purpose

    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Alive
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Wt gene

    Promoter

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. Luciferase-pcDNA3

    Plasmid

    #18964

    Depositor

    William Kaelin

    Article

    Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

    Insert

    Firefly Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGIP

    Plasmid

    #26777

    Purpose

    Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    EF1a-GFP-IRES-Puro (gfp )
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 2C-EGFP

    Plasmid

    #69071

    Purpose

    Expresses EGFP under MERVL_LTR

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. Str-KDEL_neomycin

    Plasmid

    #65306

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
    

    Use

    Tags

    KDEL motif and Signal Sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET28-MBP-TEV

    Plasmid

    #69929

    Purpose

    bacterial expression of MBP-TEV n-terminal fusions

    Depositor

    Zita Balklava, Thomas Wassmer

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    MBP and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Jan. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid

    #73796

    Purpose

    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA

    Depositor

    Ronald Davis

    Article

    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1

    Inserts

    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    

    Use

    CRISPR

    Tags

    Mxi1 and NLS

    Expression

    Yeast

    Mutation

    D10A, H840A

    Promoter

    pGPM1, pRPR1(TetO), and pTef1

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKSI-1

    Plasmid

    #51725

    Purpose

    cloning backbone

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBb(RSF1010)1k-GFPuv

    Plasmid

    #106395

    Purpose

    IPTG-inducible trc promoter expressing gfpuv on RSF1010 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-myc3-NEDD8

    Plasmid

    #19943

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    NEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

27. GFP-TREX1(D18N)

    Plasmid

    #27220

    Depositor

    Judy Lieberman

    Article

    Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    D18N sequence 52-GAC changed to AAC, resulting i…

    Promoter

    Available Since

    Jan. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CMV-mito-R-GECO1

    Plasmid

    #46021

    Purpose

    Red intensiometric genetically encoded Ca2+-indicators for optical imaging

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    R-GECO1
    

    Use

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    CMV

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mito-dendra2

    Plasmid

    #55796

    Purpose

    photo-activatable mitochondrial marker for expression in mammalian cells

    Depositor

    David Chan

    Article

    Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

    Insert

    mito-Dendra2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Lenti-Hb9-RFP

    Plasmid

    #37081

    Depositor

    Fred Gage

    Article

    Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

    Insert

    Hb9 RFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Hb9

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSFFV_mNG2(11)1-10

    Plasmid

    #82610

    Purpose

    Lentiviral vector of constitutive expression of mNeonGreen2(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNeonGreen2(1-10)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    K128M, S142T, R150M, G172V and K213M

    Promoter

    SFFV

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP

    Plasmid

    #55645

    Purpose

    Cre-on/Flp-on ChR2-EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde, AAV5, AAV8, and AAV9

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-15F11-HA-mEGFP

    Plasmid

    #129590

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pPM47 (UPR-RFP CEN/ARS URA3)

    Plasmid

    #20132

    Depositor

    Feroz Papa

    Article

    Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

    Insert

    UPR-RFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p65-mNeonGreen

    Plasmid

    #127172

    Purpose

    Fluorescently tagged p65 reporter

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Insert

    GFP-RelA (RELA Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167935

    Purpose

    Inducible CRISPRi expression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    KRAB-dCas9-DHFR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    tetON

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pet30b-7M SrtA

    Plasmid

    #51141

    Purpose

    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Insert

    S.aureus SrtA 7M
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, E105K, E108Q, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCI-Neo HA-hSnoN

    Plasmid

    #10908

    Depositor

    Bob Weinberg

    Article

    Sun et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

    Insert

    SnoN (SKIL Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

39. pRDA_479

    Plasmid

    #179099

    Purpose

    BE Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    ABE8e (SpG)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. human EB1 in pEGFP N1 to express EB1-GFP (JB131)

    Plasmid

    #39299

    Depositor

    Jennifer Tirnauer, Tim Mitchison

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge