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179,521 results

Showing: 11001 - 11040 of 179521 results

  2. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    182287-plasmid-map-sequence-id-363047

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  10. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid
    #170977
    Purpose
    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.
    Depositor
    Miki Ebisuya
    Publication
    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CShroom3 (Shroom3 Mouse)
    Use
    Gateway Cloning
    Tags
    SspB and mCherry
    Mutation
    Isoform X2, deleted aminoacids 1 - 1388
    Available since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170977-plasmid-map-sequence-id-336791

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  12. pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

    Plasmid
    #161926
    Purpose
    Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG
    Depositor
    Michael Bassik, Lacra Bintu
    Publication
    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315
    Insert
    rTetR(SE-G72P)
    Use
    Lentiviral and Synthetic Biology
    Tags
    3XFLAG
    Expression
    Mammalian
    Promoter
    pEF
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    161926-plasmid-map-sequence-id-324346

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  14. H2B-mGreenLantern

    Plasmid
    #164464
    Purpose
    Localization of mGreenLantern fluorescent protein to histone 2B
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mGreenLantern
    Tags
    Human Histone 2B (D26G and V119I mutations)
    Expression
    Mammalian
    Mutation
    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…
    Promoter
    CMV
    Available since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    164464-plasmid-map-sequence-id-322386

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  24. pGBW-m4137384

    Plasmid
    #149543
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike) for VSV pseudotyping
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    H. sapiens recode
    Promoter
    CMV
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    149543-plasmid-map-sequence-id-306267

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  25. pGBW-m4046828

    Plasmid
    #145742
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    145742-plasmid-map-sequence-id-298346

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  27. pBluescript-PB

    Plasmid
    #133885
    Purpose
    piggyBac ITR's in high copy number pBluescript backbone plasmid
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Piggybac
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    133885-plasmid-map-sequence-id-274419

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  28. pAAVS1-TRE-MA4-GFP

    Plasmid
    #114380
    Purpose
    Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.
    Depositor
    Yuet Wai Kan
    Publication
    Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MLL-AF4 fusion gene (AFF1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    TRE
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    114380-plasmid-map-sequence-id-220669

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  35. TOM20*-SBP-GFP

    Plasmid
    #120173
    Purpose
    Expresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)
    Depositor
    Juan Bonifacino
    Publication
    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)
    Tags
    SBP and eGFP
    Expression
    Mammalian
    Mutation
    Truncated TOM20: 1-30 aa
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120173-plasmid-map-sequence-id-247521

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  40. SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

    Plasmid
    #123615
    Purpose
    CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3(R33A/K34A)-P2A-EGFP
    Expression
    Mammalian
    Mutation
    R33A/K34A in rAPOBEC1, D10A in SpCas9
    Promoter
    CAG
    Available since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    123615-plasmid-map-sequence-id-239343

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Showing: 11001 - 11040 of 179521 results