161,273 results
-
-
pBOB-CAG-SARS-CoV2-Spike-HA
Plasmid#141347Purpose3. Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M PaoDepositorInsertSARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
UseLentiviralTagsHAExpressionMutationresynthesized with human codon optimization nucle…PromoterCAGAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-HTNC
Plasmid#13763PurposeFor expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.DepositorInsertHis-TAT-NLS-Cre
UseBaculovirusTagsHis Tag, NLS, and TATExpressionBacterialMutationPromoterp10 promoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Au1-mTOR-Wild type
Plasmid#26036DepositorInsertmammalian target of rapamycin-wild type (Mtor Rat)
UseTagsAU1ExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Csy4-PE2
Plasmid#172667PurposeEnhanced prime editing system in mammalian cells. This plasmid is used by PE3 and PE3bDepositorInsertCsy4-PE2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1
Plasmid#159905PurposeMutagenesis of Slc32a1DepositorInsertSlc32a1 (Slc32a1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Paxillin-pEGFP
Plasmid#15233DepositorInsertpaxillin (PXN Chicken)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA-humanTRAF2 (1084)
Plasmid#66932PurposeMammalian Expression of TRAF2DepositorInsertTRAF2 (TRAF2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Synthetic_Human_H2A.1
Plasmid#42634DepositorInsertHuman H2A.1 (H2AC13 Human, Synthetic)
UseTagsUntaggedExpressionBacterialMutationCodon OptimizedPromoterT7Available sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJBM4
Plasmid#225291PurposeE. coli-Campylobacter shuttle vector; TetRDepositorInsertTet(O) and its putative promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-STAT1-mGFP-P2A-Puro
Plasmid#199345Purposeexpress murine STAT1 protein fused with mGFPDepositorInsertsignal transducer and activator of transcription 1 (Stat1 Mouse)
UseLentiviralTagsmGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-Mma PylRS
Plasmid#140009PurposePlasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationPromoterEF1Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-H2BC11
Plasmid#207755PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.DepositorInsertsgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-ABE8e(V106W)
Plasmid#198547PurposeExpresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-ABE8e(V106W)
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-MacroGreen
Plasmid#160665PurposeExpresses GFP fusion of a multisite mutant of the Af1521 proteinDepositorInsertAf1521 modified synthetic construct eGFP fusion
UseTagseGFP and hexahistidine_TEV recognitionExpressionBacterialMutationPromoterLacIAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SARS-CoV-2 M
Plasmid#158078PurposeExpresses SARS-CoV-2 membrane protein in mammalian cellsDepositorInsertSARS-CoV-2 matrix (M )
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK5 T204D
Plasmid#80877Purposemammalian expression of constitutively active ALK5 T204DDepositorInsertALK5 (TGFBR1 Human)
UseTagsHAExpressionMammalianMutationT204D (constitutively active)PromoterCMVAvailable sinceAug. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP
Plasmid#188767PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
tsPurple chromoprotein
Plasmid#117848PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coliDepositorInsertpromoter, RBS, tsPurple
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYT1 reporter
Plasmid#197275PurposeEncodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYT1 and HaloTag (Syt1 Rat)
UseLentiviralTagsFLAGExpressionMutationN/APromoterhSyn (human synapsin I promoter)Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21a-MetRS-His
Plasmid#124115PurposeBacterial expression plasmid for PURE system componentsDepositorInsertMethionine--tRNA ligase (metG Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
DRD2-DuET
Plasmid#213228PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertDRD2 (DRD2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_4D5-High-CAR-mCherry_PGK_4D5-Low-SynNotch_Gal4VP64_RHL133
Plasmid#164822PurposeLentiviral vector for Gal4 inducible AntiHER2 4D5-High CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic NotchDepositorInsertsAntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor
UseLentiviralTagsmCherryExpressionMutationPromoterminiCMV and pGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)
Plasmid#139996PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-GFP-HYGRO
Plasmid#115144PurposeSafe harbor site 231 knock-in vector with GFP-HygromycinR expression cassetteDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
PM-GFP
Plasmid#21213DepositorInsertPM-GFP
UseTagsGFPExpressionMammalianMutationPM targetting sequence is Lyn (MGCIKSKRKD) (Lab …PromoterAvailable sinceJuly 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-qmTQ2-cAMP
Plasmid#225250Purposemammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMPDepositorInsertqmTQ2-cAMP
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/Flag-KIAA1267
Plasmid#29758DepositorInsertKIAA1267 (KANSL1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor
Plasmid#156432PurposeVector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.DepositorInsertCTCF, rtta3G, TetO3G (Ctcf Mouse)
UseMouse TargetingTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-mCherry
Plasmid#73464PurposeExpresses mCherry for monosynaptic circuit mappingDepositorInsertmCherry
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRNA7-mGFP
Plasmid#62629Purposemammalian expression of human Alpha7 (CHRNA7) with mEGFP fused into M3-M4 loopDepositorInserthAlpha7 (CHRNA7 Human)
UseTagsmEGFP inserted in M3-M4 loopExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEvol-MjaYRS
Plasmid#153557PurposeAmber suppression for incorporating 3-aminotyrosine to proteins in E. coliDepositorInsertsMJaYRS (first copy)
MJaYRS (second copy)
amber suppression tRNA under proK promoter
UseTagsExpressionBacterialMutationPromoterglnS and pBADAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW57-GFP-P2A-MCS (Neo)
Plasmid#89181PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pISH-beta2-microglobulin
Plasmid#15883DepositorInsertbeta2-microglobulin (B2m Mouse)
UseTagsExpressionMutationPromoterAvailable sinceJan. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator
Plasmid#158585PurposeExpresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiaeDepositorInsertmembrane localization signal-tagBFP-wrmScarlet11
UseTagstagBFPExpressionYeastMutationGGGS linkerPromoterTEFAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadDE
Plasmid#193837PurposeExpress TadDE (with SpCas9) in mammalian cellsDepositorInsertTadA-DualEditor-Cas9D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2
Plasmid#164510PurposeGreen glucose sensor with mRuby2 fusion, cytosolicDepositorHas ServiceAAV9Insert(Cyto)iGlucoSnFR-mRuby2
UseAAVTagsExpressionMutationSynthetic ConstructPromoterSynapsinAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
fabp10a:GCaMP6s; cryaa:mCherry
Plasmid#230060PurposeExpression of genetically encoded calcium indicator in zebrafish hepatocytes.DepositorInsertGCaMP6s
UseZebrafish expressionTagsExpressionMutationPromoterfabp10aAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
