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We narrowed to 6,296 results for: pcDNA 3.1

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Showing: 1401 - 1450 of 6296 results

  9. pcDNA3.1-CRISPR-Puro-sgRPL3-56

    Plasmid
    #208979
    Purpose
    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.
    Depositor
    Quanfu Ma
    Insert
    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Synthetic, Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV and U6 in tandem
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208979-plasmid-map-sequence-id-418375

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  10. pcDNA3.1+ hnRNPK myc HIS

    Plasmid
    #71662
    Purpose
    Mammalian expression of hnRNPK
    Depositor
    Gideon Dreyfuss
    Insert
    hnRNPK (HNRNPK Human)
    Tags
    HIS and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71662-plasmid-map-sequence-id-265916

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  13. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182287-plasmid-map-sequence-id-363047

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  20. pcDNA3.1-SARS2-Spike-HiBiT

    Plasmid
    #191212
    Purpose
    Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay
    Depositor
    Mohan Babu
    Article
    Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:
    Insert
    SARS-CoV-2 spike_WT (S SARS CoV-2)
    Tags
    HiBiT (high affinity small nanoluciferase fragmen…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191212-plasmid-map-sequence-id-437009

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  24. pcDNA3.1+CMV-Ins-GLuc

    Plasmid
    #89929
    Purpose
    Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Synthetic, Human)
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    CMV
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    89929-plasmid-map-sequence-id-255704

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  25. pcDNA3.1-Gαs 99-Venus (short)

    Plasmid
    #250711
    Purpose
    Expressing Venus funsed human Gαs R201C, short isoform
    Depositor
    Mikel Garcia-Marcos
    Insert
    G_s R201C-Venus (short isoform)
    Expression
    Mammalian
    Mutation
    R201C in G_s sequence
    Promoter
    CMV
    Available Since
    March 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250711-plasmid-map-sequence-id-501643

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  27. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid
    #71657
    Purpose
    mammalian expression of eiF4A1
    Depositor
    Gideon Dreyfuss
    Insert
    eiF4A1 (EIF4A1 Human)
    Tags
    HIS and myc
    Expression
    Mammalian
    Mutation
    aa403 from Ala to Arg
    Promoter
    CMV
    Available Since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71657-plasmid-map-sequence-id-422687

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  40. pcDNA3.1(-) FMC-63 scFv-Fc (huFc)

    Plasmid
    #183252
    Purpose
    Soluble expression of the murine anti-human CD19 scFv-Fc (clone FMC-63). Human IgG1 Fc.
    Depositor
    Alexander McLellan
    Insert
    anti-CD19 scFvFc (FMC-63)
    Expression
    Mammalian
    Available Since
    Oct. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183252-plasmid-map-sequence-id-369715

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Showing: 1401 - 1450 of 6296 results