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MSCVneo-HA-ER-Hoxb8

Plasmid

#222291

Purpose

The most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.

Depositor

Mark Kamps, David Sykes

Article

Wang et al Nat Methods. 2006 Apr;3(4):287-93.

Insert

ER-Hoxb8 (ESR1, Hoxb8 Human, Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

The estrogen receptor hormone binding domain cont…

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-bARGSer-AxeTxe

Plasmid

#192473

Purpose

Second-generation bacterial acoustic reporter gene derived from Serratia

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Inserts

bARGSer
AxeTxe

Expression

Bacterial

Mutation

the gene Ser39006_001280 was deleted from the clu…

Promoter

Native promoter from Enterococcus faecium and pBAD

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-Hsp-Fluc-PGK-Rluc-mCherry

Plasmid

#191863

Purpose

Heat inducible Fluc reporter with constitutive Rluc

Depositor

Yingxiao Wang

Article

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

Firefly luciferase Fluc
Renilla luciferase Rluc

Use

Lentiviral

Tags

P2A-mCherry

Expression

Mammalian

Promoter

HSPA7 promoter and PGK promoter

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-NLS-HA-Bxb1

Plasmid

#51271

Purpose

Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS-HA-Bxb1

Expression

Mammalian

Promoter

CAG

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pU6-tevopreq1-GG-acceptor

Plasmid

#174038

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pRL-SV40P

Plasmid

#27163

Purpose

Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments

Depositor

Ron Prywes

Article

Chen et al Mol Cell Biol. 1999 Jul . 19(7):4695-702

Insert

SV40 promoter

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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Tau (wt) -VC

Plasmid

#87369

Purpose

expresses hTau fused to the C-terminal part of Venus

Depositor

Tiago Outeiro

Article

Blum et al Hum Mol Genet. 2015 Jan 1;24(1):76-85. d

Insert

MAPT (MAPT Human)

Tags

c-terminal part of Venus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO eGFP Puro (w159-1)

Plasmid

#17481

Purpose

3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pUMVC

Plasmid

#8449

Purpose

Packaging plasmid for producing MuLV retroviral particles. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.

Depositor

Bob Weinberg

Article

Stewart et al RNA 2003 Apr;9(4):493-501.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only

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pEF1a-hMLH1dn

Plasmid

#174824

Purpose

Mammalian expression of human MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1dn (codon optimized)

Expression

Mammalian

Mutation

deletion of residues 754-756

Promoter

EF1a

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pscAAV-CAG-GFP

Plasmid

#83279

Purpose

recombinant AAV vector packaging self-complementary GFP under the CAG promoter

Depositor

Mark Kay

Article

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

enhanced green fluorescent protein

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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4787 TRV2-eTnpBc-HHreRNA4PDS

Plasmid

#251817

Purpose

Editing Photene desaturase (PDS) genes in Nicotiana benthamiana

Depositor

Savithramma Dinesh-Kumar

Article

Ugrappa et al Nat. Plants (2026). 20 February 2026.

Insert

Enhanced variant of ISDra2 eTnpBc with guide targeting NbPDS flanked by HH-HDV ribozymes

Tags

AtFDnls

Expression

Plant

Mutation

N4Y/R110K/V192L/L222I

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV4A

Plasmid

#224448

Purpose

Rep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYNSL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-M2rtTA

Plasmid

#20342

Purpose

Lentiviral plasmid expressing the reverse tetracycline transactivator

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

M2rtTA

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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AAVS1 pegRNA2 with trimmed attP

Plasmid

#222347

Purpose

pegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installation

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

AAVS1 attP-installation pegRNA2

Use

CRISPR

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2/2

Plasmid

#232199

Purpose

AAV packaging plasmid expressing AAV2 Rep and capsid proteins

Depositor

Melina Fan, Addgene Research Program

Insert

Rep2/Cap2

Use

AAV

Expression

Mammalian

Available Since

Sept. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-PEmax

Plasmid

#174820

Purpose

Mammalian expression of SpCas9 PEmax prime editor

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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XLone-GFP

Plasmid

#96930

Purpose

Tunable and temporal expression control of GFP

Depositor

Xiaojun Lian

Article

Randolph et al Sci Rep. 2017 May 8;7(1):1549. doi: 10.1

Insert

Green Fluorescent Protein

Expression

Mammalian

Promoter

TRE3GS

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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CRISPRoff-v2.1

Plasmid

#167981

Purpose

Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

Depositor

Luke Gilbert

Article

Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

Insert

CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)

Tags

2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

Expression

Mammalian

Promoter

CAG

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-U6-Chimeric_BB-CBh-hSpCas9

Plasmid

#42230

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Has Service

Cloning Grade DNA

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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