19,445 results

1. Str-KDEL_ST-SBP-mCherry

   Plasmid

   #65265

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOSIP-KO (KanR, 186)

   Plasmid

   #45985

   Depositor

   Drew Endy, Keith Shearwin

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBA559

   Plasmid

   #186235

   Purpose

   pTet-LbuCas13a crRNA entry vector

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pVE13300

   Plasmid

   #137715

   Purpose

   Expresses impLbCas12a in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   impLbCas12a
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-EGFP

   Plasmid

   #89684

   Purpose

   pCAG promoter driving EGFP

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-mTET2

   Plasmid

   #89735

   Purpose

   Expresses wildtype mouse TET2 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   TET2 (Tet2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. phage UbiC G3BP1-GFP-GFP

   Plasmid

   #119950

   Purpose

   Lentiviral vector expressing G3BP1-GFP fusion protein

   Depositor

   Jeffrey Chao

   Article

   Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   UbiC

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4-Vps34-Flag

   Plasmid

   #24398

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Vps34 (PIK3C3 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTRKH2

   Plasmid

   #71312

   Purpose

   Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

   Depositor

   Todd Klaenhammer, Rodolphe Barrangou

   Article

   O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

   Type

   Empty backbone

   Use

   E. coli-gram positive bacteria shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. CoV2-Spike-D614G

    Plasmid

    #177960

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCF806-shRen.713

    Plasmid

    #186712

    Purpose

    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

    Depositor

    Christof Fellmann

    Article

    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

    Insert

    shRen.713
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Human ICAM-1

    Plasmid

    #8632

    Depositor

    Timothy Springer

    Article

    Staunton et al Cell 1988 Mar 25;52(6):925-33.

    Insert

    ICAM-1 (ICAM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Str-Ii_VSVG-SBP-EGFP

    Plasmid

    #65300

    Purpose

    synchronize trafficking of VSVG from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    VSV-G
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC-BETAipi

    Plasmid

    #53277

    Purpose

    Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

    Insert

    idi
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoter

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Str-Golgin84_VSVG-SBP-EGFP

    Plasmid

    #65305

    Purpose

    synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Golgin-84 (GOLGA5 Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CaV2.1 (pcDNA6)

    Plasmid

    #26578

    Depositor

    Diane Lipscombe

    Article

    Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

    Insert

    Cacna1a (Cacna1a Mouse)
    

    Use

    Tags

    His6 and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO-p53-shRNA-941

    Plasmid

    #25637

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    p53-shRNA-941 (TP53 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. phage UbiC tagRFP-T-DDX6

    Plasmid

    #119947

    Purpose

    Lentiviral vector expressing tagRFP-T-DDX6 fusion protein

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    DDX6 (DDX6 Human)
    

    Use

    Lentiviral

    Tags

    tagRFP-T

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    UbiC

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBA707

    Plasmid

    #186238

    Purpose

    KanR-BBR1 entry vector for HR donor

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1-puro U6 sgRNA BfuAI stuffer

    Plasmid

    #50920

    Purpose

    U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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