8,866 results

1. pBA439

   Plasmid

   #85967

   Purpose

   Perturb-seq vector backbone

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-UTX-F

   Plasmid

   #17438

   Purpose

   Expression of UTX in mammalian cells

   Depositor

   Kai Ge

   Article

   Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

   Insert

   UTX (KDM6A Human)
   

   Tags

   6xMyc and Flag

   Expression

   Mammalian

   Available Since

   Feb. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.CAG.iGluSnFR3.v857.GPI

   Plasmid

   #178335

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV CAG iGluSnFR3 v857.GPI
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   GPI Anchor, IgK-chain, and Myc epi tag

   Expression

   Mammalian

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1099 pcDNA GFP FKHR AAA

   Plasmid

   #9023

   Depositor

   William Sellers

   Article

   Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

   Insert

   FKHR AAA (FOXO1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   T24A S256A S319A

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW-Sox10

   Plasmid

   #36978

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Sox10 (SOX10 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBOB-CAG-SARS-CoV2-Spike-HA

   Plasmid

   #141347

   Purpose

   3rd Generation lentiviral vector expressing the codon-optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

   Depositor

   Gerald Pao

   Article

   Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

   Insert

   SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   resynthesized with human codon optimization nucle…

   Promoter

   CAG

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15-UbE2D1

   Plasmid

   #15782

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
   

   Tags

   6x His, T7 tag, and Xpress tag (enterokinase clea…

   Expression

   Bacterial

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-EGFP-HaloTag2

   Plasmid

   #41742

   Depositor

   Craig Crews

   Article

   Neklesa et al Nat Chem Biol. 2011 Jul 3;7(8):538-43. d

   Insert

   Halotag 2
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-hPTGS2-2flag

   Plasmid

   #102498

   Purpose

   Mammalian expression of PTGS2

   Depositor

   Jun Yu

   Article

   Chen et al Oncogene. 2017 Aug;36(31):4415-4426. doi

   Insert

   PTGS2 (PTGS2 Human)
   

   Tags

   Flag-Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCuo CA Rac1

    Plasmid

    #84643

    Purpose

    constitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.

    Depositor

    Sanjay Kumar

    Article

    MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Constitutively Active, Q61L

    Promoter

    cumate-inducible promoter

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223-PFKFB3

    Plasmid

    #23668

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PFKFB3 (PFKFB3 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-mCherry-IRES-Flpo

    Plasmid

    #55634

    Purpose

    Expresses Flpo in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV1

    Inserts

    mCherry
    Flpo
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a and Ef1a/IRES

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. APEX2-csGBP

    Plasmid

    #108874

    Purpose

    Expression of APEX2 fused to a conditionally stable nanobody against GFP

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    APEX2-csGBP
    

    Expression

    Mammalian

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. LAMP1-GFP

    Plasmid

    #16290

    Depositor

    Ron Vale

    Article

    Minin et al J Cell Sci. 2006 Feb 15. 119(Pt 4):659-7

    Insert

    LAMP1/RE72002
    

    Tags

    EGFP

    Expression

    Insect

    Promoter

    Ac5

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHRdSV40-K560-24xGCN4_v4

    Plasmid

    #72229

    Purpose

    A fusion of the Kinesin-1 motor domain (amino acids 1-560) to the v4 SunTag peptide array

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    K560 kinesin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2-UTX-F-MT2

    Plasmid

    #40619

    Purpose

    Expression of enzyme-dead UTX in mammalian cells

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

    Insert

    UTX (KDM6A Human)
    

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Mutation

    changed Histidine 1146 and Glutamic Acid 1148 to …

    Promoter

    CMV

    Available Since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

17. 1098 pcDNA GFP FKHR

    Plasmid

    #9022

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR (FOXO1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLOVE-miR302/367

    Plasmid

    #31310

    Depositor

    Ed Morrisey

    Article

    Anokye-Danso et al Cell Stem Cell. 2011 Apr 8. 8(4):376-88.

    Insert

    mmu-miR302/367
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Oct. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pYESir2-puro

    Plasmid

    #1769

    Depositor

    Bob Weinberg

    Article

    Vaziri et al Cell 2001 Oct 19;107(2):149-59.

    Insert

    Sir2 (SIRT1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-DIO SwiChRca-TS-EYFP

    Plasmid

    #55631

    Purpose

    Cre-Dependent Chloride Channel

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Science. 2014 Apr 25;344(6182):420-4. do

    Insert

    iC1C2-TS-EYFP
    

    Use

    AAV and Cre/Lox

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C128A

    Promoter

    Ef1a

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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