8,843 results

1. Human ICAM-1

   Plasmid

   #8632

   Depositor

   Timothy Springer

   Article

   Staunton et al Cell 1988 Mar 25;52(6):925-33.

   Insert

   ICAM-1 (ICAM1 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-hM3D(Gq)-mCherry

   Plasmid

   #50474

   Purpose

   Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM3D(Gq)-mCherry (CHRM3 Human)
   

   Use

   AAV

   Tags

   mCherry

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Eco

   Plasmid

   #35617

   Depositor

   Arthur Nienhuis, Patrick Salmon

   Insert

   Ecotropic MLV envelope
   

   Expression

   Mammalian

   Available Since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRT043-CLYBL-DDdcas9-VPH

   Plasmid

   #158091

   Purpose

   Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

   Depositor

   Martin Kampmann

   Article

   Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

   Insert

   CLYBL-CAG-DDdCas9VPH-T2A-GFP
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64

   Plasmid

   #203348

   Purpose

   Template for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

   Depositor

   Christopher Grigsby, Molly Stevens

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Template for t7-mediated in vitro transcription o…

   Tags

   Flanked by 5' and 3' UTRs from Xenopus …

   Promoter

   T7

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tet-pLKO-puro-Scrambled

   Plasmid

   #47541

   Purpose

   Tet inducible scrambled shRNA.

   Depositor

   Charles Rudin

   Article

   Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

   Insert

   Scrambled shRNA
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lact-C2-GFP

   Plasmid

   #22852

   Depositor

   Sergio Grinstein

   Article

   Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

   Insert

   Lactadherin-C2 Domain (MFGE8 Bovine)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Jan. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-SFFV-GFP1-10

   Plasmid

   #80409

   Purpose

   Expresses GFP1-10 in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP1-10
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-UbC-rtTA-Ngn2:2A:EGFP

   Plasmid

   #127288

   Purpose

   all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

   Depositor

   Fred Gage

   Article

   Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

   Insert

   Ngn2:2A:Ascl1 (NEUROG2 Human)
   

   Use

   Lentiviral

   Tags

   Ngn2 and EGFP linked via 2A

   Expression

   Mammalian

   Available Since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV_BE4max

    Plasmid

    #112093

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    BE4max
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Laconic/pcDNA3.1(-)

    Plasmid

    #44238

    Purpose

    a genetically-encoded sensor for lactate concentration, transport and metabolic flux

    Depositor

    Luis Felipe Barros

    Article

    San Martin et al PLoS One. 2013;8(2):e57712. doi: 10.1371

    Insert

    Laconic (LACtate Optical Nano Indicator from CECs)
    

    Use

    Lactate biosensor

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBKT7-GW

    Plasmid

    #61703

    Purpose

    Gateway compatible Y2H vector - BD

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-NT*-HRV3CP

    Plasmid

    #162795

    Purpose

    Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

    Depositor

    Gottfried Otting

    Article

    Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

    Insert

    Human rhinovirus 14 3C protease
    

    Tags

    NT* solubility tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGADT7-GW

    Plasmid

    #61702

    Purpose

    Gateway compatible Y2H vector - AD

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. PIEZO1-HaloTag

    Plasmid

    #207834

    Purpose

    PIEZO1 fusion for biochemistry and live imaging

    Depositor

    Charles Cox

    Article

    Zhou et al Science. 2023 Aug 18;381(6659):799-804.

    Insert

    PIEZO1 (PIEZO1 Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PE6c

    Plasmid

    #207853

    Purpose

    Mammalian expression of PE6c prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6c
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTetON-EGFP

    Plasmid

    #89453

    Purpose

    Tet/Dox-inducible expression of EGFP in mammalian cells (control)

    Depositor

    Brad Zuchero

    Article

    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-EF1a-dCas9-VPR-Puro

    Plasmid

    #99373

    Purpose

    3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    (Sp)dCas9-VPR-P2A-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEvol-pAcFRS.2.t1

    Plasmid

    #73544

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAcFRS.2.t1
    pAcFRS.2.t1
    

    Expression

    Bacterial

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-pegRNA-GG-acceptor

    Plasmid

    #132777

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    Exchangeable cassette
    

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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