8,834 results

1. MLM3636

   Plasmid

   #43860

   Purpose

   guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter

   Depositor

   Keith Joung

   Insert

   None
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW57.1

   Plasmid

   #41393

   Purpose

   3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro

   Depositor

   David Root

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEVOL-pBpF

   Plasmid

   #31190

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.

   Depositor

   Peter Schultz

   Article

   Chin et al Proc Natl Acad Sci U S A. 2002 Aug 20;99

   Insert

   M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
   

   Expression

   Bacterial

   Mutation

   Y32G, E107P, D158T, I159S

   Available Since

   Dec. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMIG-FLAG-MLL-AF9

   Plasmid

   #71443

   Purpose

   Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFP

   Depositor

   Daisuke Nakada

   Article

   Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

   Insert

   MLL-Af9 (KMT2A Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-EF1-Luc2-P2A-tdTomato

   Plasmid

   #72486

   Purpose

   Express Luc2 and tdTomato under EF1 promoter

   Depositor

   Kazuhiro Oka

   Insert

   Luc2-P2A-tdTomato
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. CRISPRoff-v2.1

   Plasmid

   #167981

   Purpose

   Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
   

   Tags

   2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSETB/mStayGold

   Plasmid

   #212017

   Purpose

   mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   mStayGold
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. P4-PE

   Plasmid

   #211375

   Purpose

   Packaging plasmid for v3b PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   P4-PE
   

   Expression

   Mammalian

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-GFP(1-10)

   Plasmid

   #70219

   Purpose

   Expresses GFP(1-10) in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Has Service

   Cloning Grade DNA

   Insert

   GFP(1-10)
   

   Expression

   Mammalian

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti-EF1a-dCas9-VPR-Puro

    Plasmid

    #99373

    Purpose

    3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    (Sp)dCas9-VPR-P2A-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJC175e

    Plasmid

    #79219

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PPSP gIII, luxAB
    

    Expression

    Bacterial

    Promoter

    PPSP

    Available Since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-dCas9-KRAB-blast

    Plasmid

    #89567

    Purpose

    Plasmid expression dCas9 protein in fusion with KRAB domain

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    Blasticidin resistance gene and KRAB

    Mutation

    D10A and H840A

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHCMV_Bless

    Plasmid

    #207255

    Purpose

    Mammalian expression of Baboon Endogenous Retrovirus env protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    BaboonEndogenousRetrovirus_Rless_env
    

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-MAPPER

    Plasmid

    #117721

    Purpose

    MAPPER Selectively Labels ER-PM Junctions

    Depositor

    Jen Liou

    Article

    Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

    Insert

    MAPPER
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-GalT

    Plasmid

    #11929

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Cole et al Science. 1996 Aug 9. 273(5276):797-801.

    Insert

    GalT (GALT Human)
    

    Expression

    Mammalian

    Mutation

    Amino acids 1-60 of the human galactosyltransfera…

    Available Since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. SIRT6 Flag

    Plasmid

    #13817

    Purpose

    Mammalian expression of human SIRT6 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT6 (SIRT6 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-Lifeact-7

    Plasmid

    #54491

    Purpose

    Localization: Actin, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    mCherry-Lifeact
    

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS-CMV-gagpol

    Plasmid

    #35614

    Depositor

    Patrick Salmon

    Insert

    MLV gag and pol genes
    

    Expression

    Mammalian

    Available Since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. gag-COM-pol

    Plasmid

    #211373

    Purpose

    Packaging plasmid for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MMLVgag-COM-pol
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPRia-v2

    Plasmid

    #84832

    Purpose

    CRISPRi/a V2 library parental plasmid

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Inserts

    sgRNA
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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