8,835 results

1. lentiCRISPR v2-Blast

   Plasmid

   #83480

   Purpose

   Mammalian expression of Cas9 and sgRNA scaffold

   Depositor

   Mohan Babu

   Insert

   Blasticidin S deaminase
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   EF-1α

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiGuide-Puro

   Plasmid

   #52963

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Cloning Grade DNA

   Inserts

   S. pyogenes sgRNA cassette
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Ef1-a and hU6

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-TALEN-L

   Plasmid

   #59025

   Purpose

   TALEN designed to target the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

   Insert

   AAVS1-TALEN-L
   

   Use

   AAV and TALEN

   Tags

   3xFlag and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-TALEN-R

   Plasmid

   #59026

   Purpose

   TALEN designed to target the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

   Insert

   AAVS1-TALEN-R
   

   Use

   AAV and TALEN

   Tags

   3xFlag and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-GfaABC1D-Cre-4x6T

   Plasmid

   #196410

   Purpose

   Astrocytic expression of Cre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Has Service

   AAV5

   Insert

   Cre
   

   Use

   AAV; Astrocyte-selective

   Expression

   Mammalian

   Promoter

   CAG and GfaABC1D

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pInducer20

   Plasmid

   #44012

   Purpose

   Tet-inducible lentiviral vector for ORF expression

   Depositor

   Stephen Elledge

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)Flag-His-ATM wt

   Plasmid

   #31985

   Purpose

   Mammalian expression of human ATM with Flag and His tags

   Depositor

   Michael Kastan

   Article

   Canman et al Science. 1998 Sep 11;281(5383):1677-9.

   Insert

   ATM wild-type (ATM Human)
   

   Tags

   Flag and HIS

   Expression

   Mammalian

   Mutation

   3' UTR of 400 nucl.

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSIRV-AP-1-mCherry

   Plasmid

   #118095

   Purpose

   Fluorescent reporter for AP-1 activation

   Depositor

   Peter Steinberger

   Article

   Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

   Insert

   mCherry
   

   Use

   Retroviral

   Promoter

   minimal promoter with AP-1 responsive element

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-PGK-Neo-PIP-FUCCI

   Plasmid

   #118616

   Purpose

   Lentiviral expression of the cell cycle marker PIP-FUCCI

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   PIP-FUCCI
   

   Use

   Lentiviral

   Tags

   mCherry and mVenus

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. LT3GEPIR

    Plasmid

    #111177

    Purpose

    Tet-ON miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUdeltaGW-rtTA

    Plasmid

    #19780

    Purpose

    3rd generation lentiviral vector

    Depositor

    Konrad Hochedlinger

    Article

    Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

    Insert

    reverse tetracycline transactivator
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCTcon2

    Plasmid

    #41843

    Depositor

    Dane Wittrup

    Article

    Chao et al Nat Protoc. 2006;1(2):755-68.

    Insert

    CD20
    

    Tags

    HA and c-myc

    Expression

    Yeast

    Available Since

    Jan. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTet-O-Ngn2-puro

    Plasmid

    #52047

    Purpose

    3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

    Depositor

    Marius Wernig

    Article

    Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

    Insert

    Ngn2 (Neurog2 Mouse)
    

    Use

    Lentiviral

    Tags

    T2A-and puromycin resistant cassette

    Expression

    Mammalian

    Promoter

    tet-on

    Available Since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVS1-tet-iCas9-BFP2

    Plasmid

    #125519

    Purpose

    all-in-one-inducible Cas9

    Depositor

    Jason Moffat

    Article

    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

    Insert

    AAVS1-TET-ON 3G-SpCas9-P2A-BFP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPRv2 blast

    Plasmid

    #98293

    Purpose

    Variant of lentiCRISPRv2 that confers blasticidin S resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    EF-1a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode

    Plasmid

    #170255

    Purpose

    Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    SV40 Large T Antigen
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-PE2

    Plasmid

    #132775

    Purpose

    Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE2
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PIEZO1-HaloTag

    Plasmid

    #207834

    Purpose

    PIEZO1 fusion for biochemistry and live imaging

    Depositor

    Charles Cox

    Article

    Zhou et al Science. 2023 Aug 18;381(6659):799-804.

    Insert

    PIEZO1 (PIEZO1 Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AbVec2.0-IGHG1

    Plasmid

    #80795

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid

    #140002

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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