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pAAV2/1

Plasmid

#112862

Purpose

AAV packaging plasmid expressing Rep/Cap genes

Depositor

James M. Wilson

Insert

Rep2/Cap1

Use

AAV

Promoter

Truncated P5

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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phU6-gRNA

Plasmid

#53188

Purpose

Expresses the S. pyogenes sgRNA from the human U6 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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35S:RUBY

Plasmid

#160908

Purpose

RUBY under the control of CaMV 35S promoter/marker for transformation

Depositor

Yunde Zhao

Article

He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

Insert

RUBY and CaMV 35S promoter

Expression

Plant

Promoter

CaMV 35S

Available Since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pScaf

Plasmid

#111401

Purpose

Phagemid for producing DNA origami scaffolds, derived from pUC18 and M13mp18

Depositor

Shawn Douglas

Article

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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phH1-gRNA

Plasmid

#53186

Purpose

Expresses the S. pyogenes sgRNA from the H1 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human H1

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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v5 ABE-eVLP

Plasmid

#228467

Purpose

Expresses MMLVgag(C507V)–ABE8e for producing v5 ABE-eVLPs

Depositor

David Liu

Article

Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

Insert

MMLVgag(C507V)–ABE8e

Tags

FLAG

Expression

Mammalian

Mutation

MMLVgag(C507V)

Promoter

CMV

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pmU6-gRNA

Plasmid

#53187

Purpose

Expresses the S. pyogenes sgRNA from the mouse U6 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

mouse U6

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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ph7SK-gRNA

Plasmid

#53189

Purpose

Expresses the S. pyogenes sgRNA from the 7SK promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human 7SK

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-ccdB

Plasmid

#14155

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pLV GG hUbC-dsRED

Plasmid

#84034

Purpose

Contains Golden Gate cassette to express up to four gRNAs and dsRED

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pRL-SV40P

Plasmid

#27163

Purpose

Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments

Depositor

Ron Prywes

Article

Chen et al Mol Cell Biol. 1999 Jul . 19(7):4695-702

Insert

SV40 promoter

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pU6-tevopreq1-GG-acceptor

Plasmid

#174038

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-OVA

Plasmid

#64599

Purpose

Expression of chicken ovalbumin as model antigen for study of MHC class I and class II presentation

Depositor

Sandra Diebold, Martin Zenke

Article

Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Insert

Ovalbumin

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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evoBxb1

Plasmid

#222338

Purpose

Plasmid for expressing evolved Bxb1 in the mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved Bxb1

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSIJ8

Plasmid

#68122

Purpose

Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

Depositor

Alex Nielsen

Article

Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

Inserts

flp recombinase
rhaS
rhaR

Expression

Bacterial

Promoter

rhamnose

Available Since

Dec. 8, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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lentiCRISPR v2

Plasmid

#52961

Purpose

Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Inserts

Cas9
Puromycin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS

Available Since

May 8, 2014

Availability

Academic Institutions and Nonprofits only

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HP17_KO7

Plasmid

#120346

Purpose

Helper plasmid for production of phagemid ssDNA, based on M13KO7

Depositor

Hendrik Dietz

Article

Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

Insert

Coding part of the M13KO7 helper phage genome

Expression

Bacterial

Promoter

native phage promoters

Available Since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pmiRFP680-N1

Plasmid

#136557

Purpose

A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP680 (another name miRFP713V254C)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

miRFP680

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GenEPi

Plasmid

#140236

Purpose

Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

Depositor

Periklis Pantazis

Article

Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

Insert

GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)

Tags

GCaMP 6s RS1 EF4

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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lenti U6-sgRNA/EF1a-mCherry

Plasmid

#114199

Purpose

Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

sgRNA scaffold

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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