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We narrowed to 6,692 results for: GFP expression plasmids

Showing: 2201 - 2250 of 6692 results

  3. pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)

    Plasmid
    #140004
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140004-plasmid-map-sequence-id-275398

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  4. GFP-C1-PLCdelta-PH

    Plasmid
    #21179
    Purpose
    encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids
    Depositor
    Tobias Meyer
    Article
    Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.
    Insert
    PLCdelta (PLCD1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    PH domain of human PLC delta, amino acids Met1–Il…
    Promoter
    Available since
    July 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21179-plasmid-map-sequence-id-212104

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  7. EGFP–COSMO–PB

    Plasmid
    #169684
    Purpose
    COSMO responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Use
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y104W
    Promoter
    CMV
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    169684-plasmid-map-sequence-id-338138

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  9. pWPT-mEGFP

    Plasmid
    #190606
    Purpose
    Obtained by creating a deletion inside the mCherry coding sequence in pWPT-/GCCACC-mEGFP-IRES-mCherry (Addgene #49235)
    Depositor
    Alessandro Quattrone
    Article
    Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    a deletion was created in mCherry CDS impairing i…
    Promoter
    EIF1-short
    Available since
    Oct. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    190606-plasmid-map-sequence-id-375284

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  10. EGFP–acVHH–PB

    Plasmid
    #169685
    Purpose
    An anti-caffeine nanobody responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody
    Use
    Tags
    modified STIM1-PB tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    169685-plasmid-map-sequence-id-338140

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  13. KRD2_HBA1(HBA1reg-UbC-GFP)

    Plasmid
    #232401
    Purpose
    AAV production plasmid for HBA1 WGR vector from Fig. 1 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBA1 UTRs flank UbC-GFP cassette; GFP is followed by BGH polyA. HAs are ~400bp each.
    Depositor
    Kyle Cromer, Matthew Porteus
    Article
    Cromer et al Nat Med. 2021 Apr;27(4):677-687. doi: 10
    Insert
    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232401-plasmid-map-sequence-id-462745

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  14. pGRG36-Kn-PA1-GFP

    Plasmid
    #79088
    Purpose
    pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)
    Depositor
    Frank Rosenzweig
    Article
    Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)
    Insert
    GFP
    Use
    Bacterial chromosome integration and expression o…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PA1
    Available since
    July 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    79088-plasmid-map-sequence-id-182383

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  18. FUGW-d2GFP-200

    Plasmid
    #79602
    Purpose
    FUGW lentiviral vector with constiutively expressed destabilized GFP (d2GFP) regulated by 5 miR-200 family binding sites
    Depositor
    Jeffrey Rosen
    Article
    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.
    Insert
    d2GFP + miR-200 family targets
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    79602-plasmid-map-sequence-id-152507

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  19. pBbB8k-csg-NbGFP

    Plasmid
    #166858
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469
    Insert
    csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166858-plasmid-map-sequence-id-329027

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  34. ADEPT-pTarget-GFP

    Plasmid
    #238041
    Purpose
    Encodes sfGFP under lac promoter. Expresses a single guide RNA (under Rha promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.
    Depositor
    Lingchong You
    Article
    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/
    Insert
    sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    lac
    Available since
    June 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    238041-plasmid-map-sequence-id-476072

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  40. pAAV/L7-6-GFP-WPRE

    Plasmid
    #126462
    Purpose
    An AAV vector that expresses GFP under the control of the L7-6 promoter. The L7-6 promoter is the short size (0.8-kb) and has highly specificity to Purkinje cells.
    Depositor
    Hirokazu Hirai
    Article
    Nitta et al Mol Ther Methods Clin Dev. 2017 Jul 27;6
    Insert
    L7-6, Purkinje cell specific promoter
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    126462-plasmid-map-sequence-id-244965

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  41. scFLARE1-p2A-eGFP

    Plasmid
    #158699
    Purpose
    Mammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection marker
    Depositor
    Alice Ting
    Article
    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p
    Insert
    scFLARE1-p2A-eGFP
    Use
    AAV
    Tags
    Flag, V5. eGFP
    Expression
    Mammalian
    Mutation
    Truncated form of TEV protease (220-242 amino aci…
    Promoter
    CMV
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158699-plasmid-map-sequence-id-315073

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  49. pMTS-hFluc-HA-GFP11

    Plasmid
    #177726
    Purpose
    Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting with an HA tag and the eleventh β-strand of GFP added to the C-terminus
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    hFluc
    Use
    Tags
    GFP11, HA, and Mitochondrial Targeting Sequence
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177726-plasmid-map-sequence-id-352620

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  50. CD8a-f-CTS-EGFP

    Plasmid
    #86063
    Purpose
    A mammalian expression plasmid encoding fibrocystin mimentic clone CD8a-f-CTS (CD8a luminal and transmembrane domains, fibrocystin ciliary targeting signal domains) with C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Fibrocystin (Pkhd1 Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains CD8a luminal and transmembrane domains a…
    Promoter
    CMV
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86063-plasmid-map-sequence-id-177944

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Showing: 2201 - 2250 of 6692 results