176,436 results

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PGKneoF2L2DTA

Plasmid

#13445

Depositor

Philippe Soriano

Publication

Hoch et al Development. 2006 Feb . 133(4):663-73.

Insert

PGKneoF2L2DTA

Use

Cre/Lox

Expression

Mammalian

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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NFAT/AP-1 3x luciferase

Plasmid

#11783

Depositor

Anjana Rao

Publication

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

NFAT/AP-1 3x (Il2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pCMV-PEmax-P2A-BSD

Plasmid

#174821

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-BSD

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRepCap6

Plasmid

#110770

Purpose

pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.

Depositor

David Russell

Publication

Rutledge et al J Virol. 1998 Jan;72(1):309-19.

Insert

AAV6 cap, AAV6 rep genes

Use

AAV

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pSBtet-Pur

Plasmid

#60507

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance gene

Depositor

Eric Kowarz

Publication

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

TCE & RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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pcDNA-Wnt3A

Plasmid

#35908

Depositor

Marian Waterman

Publication

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pCS2TAL3-RR

Plasmid

#37276

Purpose

TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)

Depositor

David Grunwald

Publication

Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

Type

Empty backbone

Tags

C terminal Tal From pTAL3, FokI RR, HA, and N-ter…

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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Dyn2-pmCherryN1

Plasmid

#27689

Depositor

Christien Merrifield

Publication

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Dynamin 2 (DNM2 Human)

Tags

mCherry

Expression

Mammalian

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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GFP-UtrCH

Plasmid

#26737

Depositor

William Bement

Publication

Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

Insert

Utrophin

Use

Reporter for f-actin

Tags

GFP

Expression

Mammalian

Mutation

Calponin homology domain of utrophin (aa# 1-261).…

Promoter

CMV IE94

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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pcDNA3.3_eGFP

Plasmid

#26822

Depositor

Derrick Rossi

Publication

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-eGFP-3'UTR

Expression

Mammalian

Available since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

12 Articles

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hIR-GFP

Plasmid

#22286

Depositor

Joseph Bass

Publication

Ramos et al Proc Natl Acad Sci U S A. 2007 Jun 19. 1

Insert

INSR insulin receptor (INSR Human)

Tags

GFP

Expression

Mammalian

Available since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pDEST53-LRRK2-G2019S

Plasmid

#25045

Depositor

Mark Cookson

Publication

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Glycine 2019 mutated to Serine

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pENTR1A-GFP-N2 (FR1)

Plasmid

#19364

Purpose

Gateway entry vector for C-term eGFP fusion, frame N2

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced Green Fluorescent Protein

Use

Gateway Cloning

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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pCDNA3-Flag MKK6(glu)

Plasmid

#13518

Depositor

Roger Davis

Publication

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK6(glu) (MAP2K6 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser-207Glu Thr-211Glu

Promoter

CMV

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

33 Articles

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SIRT4 Flag

Plasmid

#13815

Purpose

Mammalian expression of human SIRT4 with flag tag

Depositor

Eric Verdin

Publication

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT4 (SIRT4 Human)

Tags

flag

Expression

Mammalian

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pLV-tTRKRAB-red

Plasmid

#12250

Depositor

Didier Trono

Publication

Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

Insert

TetR-KRAB fusion and dsRED

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Mutation

dsRed added to backbone.

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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Ub-R-GFP

Plasmid

#11939

Depositor

Nico Dantuma

Publication

Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

Insert

Ub-R-GFP

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Arginine at…

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pcDNA3-SARS-CoV-2-S-RBD-8his

Plasmid

#145145

Purpose

Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

Depositor

Erik Procko

Publication

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)

Tags

8his tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pSIRV-NF-kB-eCFP

Plasmid

#118094

Purpose

Flourescent reporter for NF-kB activity

Depositor

Peter Steinberger

Publication

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

eCFP

Use

Retroviral

Promoter

minimal promoter with NF-kB responsive element

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AIO-GFP

Plasmid

#74119

Purpose

All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

Depositor

Steve Jackson

Publication

Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pCMV-BE2

Plasmid

#73020

Purpose

Expresses BE2 in mammalian cells

Depositor

David Liu

Publication

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE2

Expression

Mammalian

Promoter

CMV

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pDestTol2pA2-U6:gRNA

Plasmid

#63157

Purpose

Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.

Depositor

Leonard Zon

Publication

Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

Type

Empty backbone

Use

CRISPR; Tol2 destination vector for gateway

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAV-EF1a-Cre

Plasmid

#55636

Purpose

Expresses Cre in Mammalian Cells

Depositor

Karl Deisseroth

Publication

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has service

AAV11 and AAV Retrograde

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Ef1a

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

47 Articles

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hAAVS1 1L TALEN

Plasmid

#35431

Purpose

TALEN designed to target the human AAVS1 locus

Depositor

Feng Zhang

Publication

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

hAAVS1 1L TALEN

Expression

Mammalian

Available since

May 17, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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hAAVS1 1R TALEN

Plasmid

#35432

Purpose

TALEN designed to target the human AAVS1 locus

Depositor

Feng Zhang

Publication

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

hAAVS1 1R TALEN

Expression

Mammalian

Available since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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pCXLE-hOCT3/4

Plasmid

#27076

Purpose

Non-integrating (episomal) expression of human OCT3/4

Depositor

Shinya Yamanaka

Publication

Okita et al Nat Methods. 2011 May;8(5):409-12

Insert

OCT3/4 (POU5F1 Human)

Expression

Mammalian

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

53 Articles

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XBP-1 2: pFLAG.XBP1p.CMV2

Plasmid

#21833

Depositor

David Ron

Publication

Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

Insert

XBP-1 processed/spliced (Xbp1 Mouse)

Tags

Flag

Expression

Mammalian

Available since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pcDNA3-myc3-CUL4A

Plasmid

#19951

Depositor

Yue Xiong

Publication

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CUL4A (cullin 4A) (CUL4A Human)

Tags

myc3

Expression

Mammalian

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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HA-Akt DN (K179M)

Plasmid

#16243

Depositor

Mien-Chie Hung

Publication

Zhou et al J Biol Chem. 2000 Mar 17. 275(11):8027-3

Insert

Akt1 (Akt1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (K179M)

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pcDNA3-EGFP-Cdc42-T17N

Plasmid

#12976

Depositor

Gary Bokoch

Publication

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Cdc42 DN (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T17N Dominant negative

Promoter

CMV

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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