4,167 results

1. pCA24N-ligase

   Plasmid

   #87741

   Purpose

   IPTG-inducible expression of T4 DNA ligase for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T5-lac

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCRIS-PITChv2-BSD-dTAG (BRD4)

   Plasmid

   #91792

   Purpose

   PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

   Depositor

   Behnam Nabet, James Bradner

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   BSD-P2A-2xHA-FKBP_F36V
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promoterless

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. T7-VEE-IRES-Puro

   Plasmid

   #58970

   Purpose

   noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Type

   Empty backbone

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Expression

   Mammalian

   Promoter

   26S subgenomic promoter

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET15b-AavLEA1

   Plasmid

   #53093

   Purpose

   AavLEA1 expression (in BL21 DE3 E. coli strain)

   Depositor

   Claude Férec

   Article

   Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

   Insert

   AavLEA1
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pgRNA-bacteria

   Plasmid

   #44251

   Purpose

   Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   guide RNA (bacteria)
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   BBa_J23119 (SpeI)

   Available Since

   April 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCS2+HyPer7-NES

   Plasmid

   #136467

   Purpose

   Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Tags

   Nuclear export signal from the HIV Rev protein

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE

   Plasmid

   #135391

   Purpose

   Cre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.

   Depositor

   Fan Wang

   Insert

   TeLC-P2A-EYFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CMV-mCherry-(F30-2xPepper)10

   Plasmid

   #129401

   Purpose

   mCherry mRNA reporter tagged with (F30-2xPepper)10

   Depositor

   Samie Jaffrey

   Article

   Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

   Insert

   mCherry-(F30-2xPepper)10
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. tau/pET29b

   Plasmid

   #16316

   Depositor

   Peter Klein

   Article

   Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

   Insert

   tau (MAPT Human)
   

   Expression

   Bacterial

   Available Since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDestTol2CG2-U6:gRNA

    Plasmid

    #63156

    Purpose

    Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Type

    Empty backbone

    Use

    CRISPR; Tol2 destination vector for gateway

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pscAAV-GFP

    Plasmid

    #32396

    Depositor

    John T Gray

    Article

    Gray et al Methods Mol Biol. 2011;807:25-46.

    Insert

    GFP
    

    Use

    AAV; Adeno-associated virus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PCS2+Cas9-mSA

    Plasmid

    #103882

    Purpose

    Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    CAS9-MSA
    

    Use

    CRISPR

    Tags

    MSA (monomeric streptavidin

    Mutation

    none

    Promoter

    SP6

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-MmPeg10rc4

    Plasmid

    #174858

    Purpose

    Expresses recoded mouse Peg10 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    recoded Mus musculus PEG10 (Peg10 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMXs-3XMyc-EGFP-OMP25

    Plasmid

    #83355

    Purpose

    For tagging mitochondria with Myc epitopes (Control-MITO construct)

    Depositor

    David Sabatini

    Article

    Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

    Insert

    3XMyc-EGFP-OMP25
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CRE recombinase

    Plasmid

    #62730

    Purpose

    Expresses Cre recombinase protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    Cre recombinase
    

    Use

    Cre/Lox

    Tags

    His tag and NLS

    Expression

    Bacterial

    Mutation

    Codon optimazed for e.coli

    Promoter

    T7

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti6.2_mTagBFP2

    Plasmid

    #113725

    Purpose

    Expresses the fluorescent protein mTagBFP2 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Article

    Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

    Insert

    mTagBFP2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LentiGuide-Hygro

    Plasmid

    #139462

    Purpose

    Lentiviral vector with gRNA scaffold and hygromycin selectable marker

    Depositor

    Caroline Goujon

    Insert

    no sgRNA inserted; resistance gene: hygroR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-mRFP

    Plasmid

    #13032

    Purpose

    Mammalian expression vector for cloning your gene fused to mRFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Insert

    Monomer Red Fluorescent Protein
    

    Expression

    Mammalian

    Available Since

    Nov. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENN.AAV.hSyn.Cre.WPRE.hGH

    Plasmid

    #105553

    Purpose

    AAV expression of Cre from hSyn promoter

    Depositor

    James M. Wilson

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCW57-MCS1-P2A-MCS2 (GFP)

    Plasmid

    #80924

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge