19,728 results

1. pAAV.CAG.iGluSnFR3.v857.GPI

   Plasmid

   #178335

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV CAG iGluSnFR3 v857.GPI
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   GPI Anchor, IgK-chain, and Myc epi tag

   Expression

   Mammalian

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-C1ab-Prkd1

   Plasmid

   #139314

   Purpose

   Diacylglycerol biosensor

   Depositor

   Gerry Hammond

   Article

   Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   mCherry:Mus musculus Prkd1(138-343) (Prkd1 Mouse)
   

   Expression

   Mammalian

   Mutation

   T208A, N313D

   Available Since

   April 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1099 pcDNA GFP FKHR AAA

   Plasmid

   #9023

   Depositor

   William Sellers

   Article

   Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

   Insert

   FKHR AAA (FOXO1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   T24A S256A S319A

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUW-Sox10

   Plasmid

   #36978

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Sox10 (SOX10 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHJ12: CadC-VHH-Caffeine (No linker)

   Plasmid

   #108244

   Purpose

   Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

   Depositor

   Jerome Bonnet

   Article

   Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

   Insert

   Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
   

   Use

   Synthetic Biology

   Promoter

   pLacO1

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-P(Per2)-DIO-intron2-Venus-NLS-D2

   Plasmid

   #110057

   Purpose

   Per2 transcription fluorescence reporter

   Depositor

   Eric Erquan Zhang

   Article

   Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

   Insert

   Per2 promoter and Venus (Per2 Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   NLS-D2

   Expression

   Mammalian

   Promoter

   Per2

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBOB-CAG-SARS-CoV2-Spike-HA

   Plasmid

   #141347

   Purpose

   3rd Generation lentiviral vector expressing the codon-optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

   Depositor

   Gerald Pao

   Article

   Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

   Insert

   SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   resynthesized with human codon optimization nucle…

   Promoter

   CAG

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOPINS-AREL1

   Plasmid

   #66710

   Purpose

   Expresses AREL1 HECT domain in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Insert

   KIAA0317 (AREL1 )
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pET15-UbE2D1

   Plasmid

   #15782

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
   

   Tags

   6x His, T7 tag, and Xpress tag (enterokinase clea…

   Expression

   Bacterial

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-EGFP-HaloTag2

    Plasmid

    #41742

    Depositor

    Craig Crews

    Article

    Neklesa et al Nat Chem Biol. 2011 Jul 3;7(8):538-43. d

    Insert

    Halotag 2
    

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-hPTGS2-2flag

    Plasmid

    #102498

    Purpose

    Mammalian expression of PTGS2

    Depositor

    Jun Yu

    Article

    Chen et al Oncogene. 2017 Aug;36(31):4415-4426. doi

    Insert

    PTGS2 (PTGS2 Human)
    

    Tags

    Flag-Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCuo CA Rac1

    Plasmid

    #84643

    Purpose

    constitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.

    Depositor

    Sanjay Kumar

    Article

    MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Constitutively Active, Q61L

    Promoter

    cumate-inducible promoter

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. hFLT1

    Plasmid

    #83435

    Purpose

    human VEGFR1 with HA and FLAG tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Exp Cell Res. 2013 Oct 15;319(17):2578-8

    Insert

    human VEGFR1 (FLT1 Human)
    

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    HA-tag was inserted 30 AA downstream of the FLT1 …

    Promoter

    CMV

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-DIO mScarlet

    Plasmid

    #131002

    Purpose

    Double floxed mScarlet under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV1 and AAV5

    Insert

    mScarlet
    

    Use

    AAV

    Promoter

    Ef1a

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-PFKFB3

    Plasmid

    #23668

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PFKFB3 (PFKFB3 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. mito-mScarlet

    Plasmid

    #141153

    Purpose

    Fluorescent labeling of the mitochondrial matrix in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    COX4 (COX4 Budding Yeast)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1a-mCherry-IRES-Flpo

    Plasmid

    #55634

    Purpose

    Expresses Flpo in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV1

    Inserts

    mCherry
    Flpo
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a and Ef1a/IRES

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pComb3

    Plasmid

    #63888

    Purpose

    For phage display of antibody libraries by fusion to modified geneIII.

    Depositor

    Carlos Barbas

    Article

    Barbas CF et al Proc Natl Acad Sci U S A. 1991 Sep 15;88

    Type

    Empty backbone

    Use

    Phage display

    Tags

    geneIII

    Expression

    Bacterial

    Promoter

    lacZ

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. APEX2-csGBP

    Plasmid

    #108874

    Purpose

    Expression of APEX2 fused to a conditionally stable nanobody against GFP

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    APEX2-csGBP
    

    Expression

    Mammalian

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. LAMP1-GFP

    Plasmid

    #16290

    Depositor

    Ron Vale

    Article

    Minin et al J Cell Sci. 2006 Feb 15. 119(Pt 4):659-7

    Insert

    LAMP1/RE72002
    

    Tags

    EGFP

    Expression

    Insect

    Promoter

    Ac5

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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