We narrowed to 115,116 results for: CIL

1. pRPF144

   Plasmid

   #106372

   Purpose

   C. difficile constitutive expression system. Shuttle plasmid containing constitutive Pcwp2 -gusA

   Depositor

   Robert Fagan, Neil Fairweather

   Article

   Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

   Insert

   Pcwp2-gusA (gusA )
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   codon optimised for C. difficile

   Promoter

   Pcwp2

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKM197

   Plasmid

   #132665

   Purpose

   pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.

   Depositor

   Joseph Sorg

   Article

   Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:

   Insert

   Upstream & Downstream pyrE deletion region
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-ciBAR1

   Plasmid

   #200440

   Purpose

   Expresses Flag-tagged human ciBAR1 in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Article

   Li et al Mol Cell Biol. 2016 Oct 13;36(21):2668-2

   Insert

   ciBAR1
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-ciBAR2

   Plasmid

   #200441

   Purpose

   Expresses Flag-tagged human ciBAR2 in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Article

   Li et al Mol Cell Biol. 2016 Oct 13;36(21):2668-2

   Insert

   ciBAR2
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK040 (pET-PurQ-A128T)

   Plasmid

   #73840

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

   Depositor

   T. J. Kappock, JoAnne Stubbe

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A128T mutant

   Promoter

   T7

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK044 (pET-PurQ-wt)

   Plasmid

   #73839

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

   Depositor

   T. J. Kappock, JoAnne Stubbe

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. phtsResA

   Plasmid

   #25914

   Depositor

   Johann Deisenhofer

   Article

   Colbert et al Proc Natl Acad Sci U S A. 2006 Mar 21. 1

   Insert

   His-tagged soluble ResA (resA Bacillus subtilis)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Start at Serine 37 to remove n-terminal transmemb…

   Promoter

   Available since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

8. BS.YxiN.Twin1.a207-368.wt

   Plasmid

   #13531

   Depositor

   David McKay

   Article

   Caruthers et al Acta Crystallograph Sect F Struct Biol C

   Insert

   B. subtilis DEAD-box helicase YxiN second domain
   

   Use

   Tags

   intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   deleted amino acid residues 1-206 and 369-479

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRPF215

   Plasmid

   #106377

   Purpose

   C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

   Depositor

   Robert Fagan, Neil Fairweather

   Article

   Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

   Insert

   Ptet-himar1-Ter(slpA)-transposon
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   Codon optimised for C. difficile

   Promoter

   Ptet

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPCK

    Plasmid

    #133128

    Purpose

    a recombinant plasmid, expressing pckA PEP-carboxykinase) gene

    Depositor

    Gregg Beckham

    Article

    Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    PEP-carboxykinase gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PEP-carboxykinase promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMDH

    Plasmid

    #133129

    Purpose

    A recombinant plasmid expressing mdh (malate dehydrogenase) gene

    Depositor

    Gregg Beckham

    Article

    Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    malate dehydrogenase gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    malate dehydrogenase promoter

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-tag

    Plasmid

    #55182

    Purpose

    HA-tag

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    HA tag
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-tag

    Plasmid

    #55180

    Purpose

    FLAG-tag

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    FLAG
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-C2gamma

    Plasmid

    #21215

    Depositor

    Tobias Meyer

    Article

    Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

    Insert

    GFP-pcDNA3-PKCgamma-c2 (Prkcg Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The C2-domain from PKCgamma (aa170-260) plus flan…

    Promoter

    Available since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. NavBp

    Plasmid

    #100014

    Purpose

    Electrophysiology in mammalian cells: Mammalian expression of a voltage gated ion channel and GFP

    Depositor

    David Clapham, Paul DeCaen

    Article

    DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

    Insert

    NavBp
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NsvBa

    Plasmid

    #100015

    Purpose

    Electrophysiology in mammalian cells: Mammalian expression of a non-selective voltage gated ion channel and GFP

    Depositor

    David Clapham, Paul DeCaen

    Article

    DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

    Insert

    NsvBa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. StrepII-tag

    Plasmid

    #55181

    Purpose

    Streptactin-tag

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    StrepII tag
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. GST-RP2

    Plasmid

    #86072

    Purpose

    A bacterial expression plasmid encoding retinitis pigmentosa 2 (RP2) with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    RP2 (RP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. GL2 shRNA

    Plasmid

    #86083

    Purpose

    A lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    control shRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. His10-tag

    Plasmid

    #55179

    Purpose

    10xHis-tag

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    His10
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge