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We narrowed to 6,995 results for: tet

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Showing: 2501 - 2550 of 6995 results

  6. pFRT-DestFLAGHA_RNAseH1

    Plasmid
    #65783
    Purpose
    constitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    RNaseH1 (RNASEH1 Human, Zebrafish)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV, 2x Tet
    Available Since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65783-plasmid-map-sequence-id-151430

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  7. pAAV-BD-hSynTet-TRE-DIO-mNeonGreen-MYC

    Plasmid
    #242789
    Purpose
    Bidirectional AAV transfer plasmid expressing hSyn-tTA and TRE-FLEX-mNeonGreen-MYC in the other direction.
    Depositor
    Andrew Payne
    Inserts
    mNeonGreen
    tTA-Advanced
    Use
    AAV
    Tags
    MYC
    Promoter
    TRE and hSyn
    Available Since
    Sept. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    242789-plasmid-map-sequence-id-486971

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  10. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

    Plasmid
    #202571
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    LINE-1
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202571-plasmid-map-sequence-id-429226

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  11. pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai

    Plasmid
    #202568
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    LINE-1
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202568-plasmid-map-sequence-id-429225

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  14. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196082
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196082-plasmid-map-sequence-id-388978

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  15. IR904: pMVP (L3-L2) pA; CMV::eGFP-P2A-TETa-pA

    Plasmid
    #121799
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven GFP-P2A-TETa downstream of gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    polyA + CMV::eGFP-P2A-TETa-polyA
    Use
    Gateway Cloning and Synthetic Biology
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121799-plasmid-map-sequence-id-237303

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  21. plRL58

    Plasmid
    #166886
    Purpose
    Optimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)
    Depositor
    Jeremy Rock
    Article
    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Tet-On
    Available Since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    166886-plasmid-map-sequence-id-339556

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  22. pTS113 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-Alfa-P2A-TagBFP

    Plasmid
    #199366
    Purpose
    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.
    Depositor
    Rebecca Voorhees
    Article
    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    3C-SUMOEu-ALFA-P2A-TagBFP
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199366-plasmid-map-sequence-id-389910

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  26. pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid
    #119946
    Purpose
    Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18
    Insert
    Renilla luciferase
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Promoter
    TetCMV
    Available Since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    119946-plasmid-map-sequence-id-237566

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  29. pTon1(il11)

    Plasmid
    #233647
    Purpose
    Expression vector of X. laevis il11.L-P2A-AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.
    Depositor
    Takeo Kubo
    Article
    Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10
    Inserts
    interleukin-11.L (350-913) (il11.L Frog)
    target sequence of guide RNA #tyr (3905-3927) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
    Use
    Amphibian expression, tet-on
    Mutation
    Some synonymous substitutions were introduced in…
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233647-plasmid-map-sequence-id-468199

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  31. pDule-para-aminoPhe

    Plasmid
    #85502
    Purpose
    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.
    Insert
    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32T, E107T, D158P, I159L, L162A
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85502-plasmid-map-sequence-id-169860

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  34. pAH-CTX1-rhadCas9

    Plasmid
    #129391
    Purpose
    Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Burkholderia cenocepacia codon-optimized dCas9
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Entire dCas9 codon optimized for the GC-rich B. c…
    Promoter
    PrhaBAD
    Available Since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    129391-plasmid-map-sequence-id-273754

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  37. tetO-HyperTRIBE-adar_deaminase-V5-t2a-mCherry

    Plasmid
    #241314
    Purpose
    inducible mouse Adar Deaminase domain (HyperTRIBE) for expression in mammalian cells
    Depositor
    Stavros Lomvardas
    Insert
    Adar deaminase domain (Adar Mouse)
    Tags
    V5
    Expression
    Mammalian
    Mutation
    E957Q
    Promoter
    tetO and CMV
    Available Since
    Oct. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    241314-plasmid-map-sequence-id-478721

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  38. pAAV-BD-hSynTet-TRE-DIO-mNeonGreen-E2

    Plasmid
    #242784
    Purpose
    Bidirectional AAV transfer plasmid expressing hSyn-tTA and TRE-FLEX-mNeonGreen-E2 in the other direction.
    Depositor
    Andrew Payne
    Inserts
    mNeonGreen
    tTA-Advanced
    Use
    AAV
    Tags
    E2
    Promoter
    TRE and hSyn
    Available Since
    Sept. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    242784-plasmid-map-sequence-id-486990

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  39. pAAV-BD-hSynTet-TRE-DIO-mNeonGreen-HA

    Plasmid
    #242786
    Purpose
    Bidirectional AAV transfer plasmid expressing hSyn-tTA and TRE-FLEX-mNeonGreen-HA in the other direction.
    Depositor
    Andrew Payne
    Inserts
    mNeonGreen
    tTA-Advanced
    Use
    AAV
    Tags
    HA
    Promoter
    TRE and hSyn
    Available Since
    Sept. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    242786-plasmid-map-sequence-id-486974

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  40. pAAV-BD-hSynTet-TRE-DIO-mNeonGreen-S1

    Plasmid
    #242793
    Purpose
    Bidirectional AAV transfer plasmid expressing hSyn-tTA and TRE-FLEX-mNeonGreen-S1 in the other direction.
    Depositor
    Andrew Payne
    Inserts
    mNeonGreen
    tTA-Advanced
    Use
    AAV
    Tags
    S1
    Promoter
    TRE and hSyn
    Available Since
    Sept. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    242793-plasmid-map-sequence-id-486968

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  41. pAAV-BD-hSynTet-TRE-DIO-mNeonGreen-T7

    Plasmid
    #242796
    Purpose
    Bidirectional AAV transfer plasmid expressing hSyn-tTA and TRE-FLEX-mNeonGreen-T7 in the other direction.
    Depositor
    Andrew Payne
    Inserts
    mNeonGreen
    tTA-Advanced
    Use
    AAV
    Tags
    T7
    Promoter
    TRE and hSyn
    Available Since
    Sept. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    242796-plasmid-map-sequence-id-486965

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  47. pSBtet-Bla/PAG1-TurboID-GAI

    Plasmid
    #198217
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Eric Davis
    Insert
    PAG1 (PAG1 Human)
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198217-plasmid-map-sequence-id-389406

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Showing: 2501 - 2550 of 6995 results