159,238 results

1. pSL1-GFP

   Plasmid

   #180420

   Purpose

   Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.

   Depositor

   Nancy Moran

   Article

   Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-G-Flamp1

   Plasmid

   #188567

   Purpose

   green biosensor for cAMP in mammalian cells

   Depositor

   Jun Chu

   Article

   Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

   Insert

   G-Flamp1
   

   Use

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. EZ-Tet-pLKO-Blast

   Plasmid

   #85973

   Purpose

   Tet/Dox inducible shRNA lentivirus with blasticidin selection

   Depositor

   Cindy Miranti

   Article

   Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   H1 / TetO

   Available since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACYC-GroEL/ES-TF

   Plasmid

   #83923

   Purpose

   Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Gro EL/ES
   Trigger Factor
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-U6-gRNA scaffold-UbC-mCherry-PuroR

   Plasmid

   #232183

   Purpose

   Lentiviral plasmid for cloning an spCas9 gRNA with puromycin resistance and mCherry

   Depositor

   Charles Gersbach

   Article

   Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, hUbC

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS1C

   Plasmid

   #55168

   Purpose

   Empty vector, integration at amyE, ampr, cmr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-mitoDsRed

   Plasmid

   #44386

   Purpose

   3rd generation lentiviral vector

   Depositor

   Pantelis Tsoulfas

   Article

   Kitay et al Hum Mol Genet. 2013 Jan 24.

   Insert

   mitoDsRed
   

   Use

   Lentiviral

   Tags

   61 aa targeting sequence of the P1 isoform F1F0-A…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-CMV-CD19 puro

   Plasmid

   #196634

   Purpose

   Express CD19

   Depositor

   Nicolas Manel

   Article

   Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

   Insert

   CD19 (CD19 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MLM3636

   Plasmid

   #43860

   Purpose

   guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter

   Depositor

   Keith Joung

   Insert

   None
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR221_SLC1A4

    Plasmid

    #131962

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC1A4 (SLC1A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. H2B-GFP

    Plasmid

    #11680

    Depositor

    Geoff Wahl

    Article

    Kanda et al Curr Biol. 1998 Mar 26. 8(7):377-85.

    Insert

    Human histone H2B (H2BC11 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 TEV (full-length)

    Plasmid

    #64276

    Purpose

    Expresses tobacco etch virus protease (TEV)

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Tobacco etch virus (TEV) protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-sCAG-mBaoJin-oPRE

    Plasmid

    #218684

    Purpose

    AAV vector plasmid expressing mBaoJin under a short variant of the CAG (sCAG) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.

    Depositor

    Bart Nieuwenhuis

    Insert

    mBaoJin
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Short variant of the CAG promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221_SLC6A19

    Plasmid

    #132231

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC6A19 (SLC6A19 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. Ecoli PancACe

    Plasmid

    #215712

    Purpose

    Expresses acetyl-CoA biosensor in Ecoli

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    PancACe
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti-UPEmS-tevo

    Plasmid

    #228894

    Purpose

    Golden gate cloning (BsmBI) of pegRNAs with tevopreQ1 motif, and with constitutive RFP expression.

    Depositor

    Francisco J Sánchez-Rivera

    Article

    Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TETv4

    Plasmid

    #167983

    Purpose

    Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter

    Depositor

    Luke Gilbert

    Article

    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

    Insert

    TETv4 (TET1CD-XTEN80-dCas9)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    3xNLS, TET1 catalytic domain, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSFFV_Gal4-VP64-IRES-mCherry (pLZA139)

    Plasmid

    #203909

    Purpose

    Encodes wild type Gal4-VP64 transcription factor in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    Gal4-VP64-IRES-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST_EWSR1

    Plasmid

    #26377

    Depositor

    Thomas Tuschl

    Article

    Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

20. mCherry-GAL9

    Plasmid

    #166689

    Purpose

    For creating a stable mCherry-GAL9 cell line

    Depositor

    Alan Sabirsh

    Article

    Munson et al Commun Biol. 2021 Feb 16;4(1):211. doi:

    Insert

    LGALS9 (LGALS9 Human)
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONR221_SLC1A2

    Plasmid

    #131901

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC1A2 (SLC1A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. AcGFP1-C1

    Plasmid

    #54607

    Purpose

    Localization: C1 Cloning Vector, Excitation: 475, Emission: 505

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLVX neo PFKP-GFP

    Plasmid

    #116940

    Purpose

    Expression of PFKP-GFP fusion protein

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    PFKP (PFKP Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. cfSGFP2-anti-AlfaTag nanobody

    Plasmid

    #171818

    Purpose

    Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

    Depositor

    Ikuo Wada

    Article

    Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

    Insert

    cfSGFP2-anti-AlfaTag nanobody
    

    Use

    Tags

    6xHis and SEP tag (6xD)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGL3 Basic Vector

    Plasmid

    #212936

    Purpose

    Firefly luciferase vector for investigating regions controlling transcription

    Depositor

    Debrya Groskreutz

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pDONR221_SLC6A15

    Plasmid

    #131877

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC6A15 (SLC6A15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pSpCas9(BB)-2A-mCherry

    Plasmid

    #197420

    Purpose

    Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pk7-GR-GFP

    Plasmid

    #15534

    Depositor

    Ian Macara

    Article

    Carey et al J Cell Biol. 1996 Jun . 133(5):985-96.

    Insert

    Glucocorticoid receptor (NR3C1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The glucocorticoid receptor was cloned into the X…

    Promoter

    Available since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR221_SLC38A3

    Plasmid

    #132142

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC38A3 (SLC38A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pDONR221_SLC7A10

    Plasmid

    #132232

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A10 (SLC7A10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pDONR221_SLC38A8

    Plasmid

    #132082

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC38A8 (SLC38A8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pPath-Violacein-native

    Plasmid

    #200001

    Purpose

    Violacein pathway. Native promoter and CDS sequence

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Pathways
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. anti-FLAG M2 light chain

    Plasmid

    #175358

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 light chain

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 light chain
    

    Use

    Tags

    N-terminal secretion signal peptide (MEAPAQLLFLLL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBABE-zeo largeTgenomic

    Plasmid

    #1778

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T and small T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNLS-iRFP670

    Plasmid

    #45466

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. anti-FLAG M2 heavy chain

    Plasmid

    #175359

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 heavy chain
    

    Use

    Tags

    His8 tag and N-terminal secretion signal peptide …

    Expression

    Mammalian

    Mutation

    L51I (see paper)

    Promoter

    CMV

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLV_3XHA-ATP6V1B2-mNeonGreen

    Plasmid

    #228865

    Purpose

    Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V1B2 (ATP6V1B2 Human)
    

    Use

    Lentiviral

    Tags

    3xHA and mNeonGreen

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLV_3xFLAG-ATP6V0A3-mScarlet

    Plasmid

    #228867

    Purpose

    Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V0A3 (TCIRG1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG and mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLV-tetO-myc T58A

    Plasmid

    #19763

    Depositor

    Konrad Hochedlinger

    Article

    Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

    Insert

    c-myc (MYC Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAG-Cre

    Plasmid

    #13775

    Purpose

    Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre
    

    Use

    Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge