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PB-CA-rtTA Adv

Plasmid

#20910

Purpose

piggyBac vector for expression of reverse tetracycline transactivator from a CAG constitutive promoter

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

rtTA-ON-Adv

Use

piggybac

Expression

Mammalian

Available Since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-CUL2

Plasmid

#19892

Depositor

Yue Xiong

Article

Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

Insert

cullin 2 (CUL2 Human)

Tags

myc3

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pHA-eIF4E

Plasmid

#17343

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

eIF4E (EIF4E Human)

Tags

HA

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pGEX-4T MDM2 WT

Plasmid

#16237

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Tags

GST

Expression

Bacterial

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pCEP4YPet-MAMM

Plasmid

#14032

Depositor

Patrick Daugherty

Article

Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

Insert

YPet

Expression

Mammalian

Mutation

Mammalian optimized YPet

Available Since

Feb. 14, 2007

Availability

Academic Institutions and Nonprofits only

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GFP-Ub KO

Plasmid

#11934

Depositor

Nico Dantuma

Article

Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

Insert

Ubiquitin KO (RBX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

All 7 lysines of ubiquitin mutated to arginines.

Available Since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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WT NFAT2

Plasmid

#11101

Depositor

Anjana Rao

Article

Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

Insert

NFAT2 (Nfatc1 Mouse)

Use

Retroviral

Tags

3xHA and GFP

Expression

Mammalian

Available Since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

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3336 pcDNA3 Bcl-2

Plasmid

#8768

Depositor

Stanley Korsmeyer

Article

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-BI30

Plasmid

#183749

Purpose

AAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with tropism for CNS endothelial cells.

Depositor

Ben Deverman

Article

Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

Insert

AAV9 modified with 7mer insertion between amino acids 588 and 589 of VP1

Use

AAV

Mutation

K449R, and NNSTRGG inserted between 588 and 589 o…

Promoter

p41

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pT4/HB

Plasmid

#108352

Purpose

Optimized Sleeping Beauty Transposon Vector with MCS

Depositor

Wolfgang Uckert

Article

Wang et al Nucleic Acids Res. 2017 Jan 9;45(1):311-

Type

Empty backbone

Use

Transposon

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry Paxillin

Plasmid

#50526

Depositor

Kenneth Yamada

Insert

PXN paxillin (PXN Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pKOV

Plasmid

#25769

Depositor

George Church

Article

Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 3, 2010

Availability

Academic Institutions and Nonprofits only

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hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

Plasmid

#24334

Depositor

Didier Stainier

Article

Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

Insert

H2B

Use

Cre/Lox

Tags

GFP

Expression

Worm

Mutation

mCherry driven by an HSP70 promoterCerulean drive…

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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FUtdTW

Plasmid

#22478

Depositor

Connie Cepko

Article

Rompani et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

tandem dimer tomato

Use

Lentiviral

Expression

Mammalian

Promoter

UbC

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 R248W

Plasmid

#16437

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 R248W (TP53 Human)

Expression

Mammalian

Mutation

R248W

Available Since

Aug. 4, 2008

Availability

Academic Institutions and Nonprofits only

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HOP-flash

Plasmid

#83467

Purpose

TEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with minimal promoter plus luciferase reporter gene

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

Multimerized TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)

Use

Luciferase

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pME pHluorin2

Plasmid

#73794

Purpose

ratiometric pHluorin2 sequence in a middle entry clone

Depositor

David Raible

Article

Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

Insert

ratiometric pHluorin2

Use

Middle entry clone

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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CRE recombinase

Plasmid

#62730

Purpose

Expresses Cre recombinase protein in bacterial cells

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

Cre recombinase

Use

Cre/Lox

Tags

His tag and NLS

Expression

Bacterial

Mutation

Codon optimazed for e.coli

Promoter

T7

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

Plasmid

#40132

Purpose

Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).

Depositor

Pawel Pelczar

Article

Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

Type

Empty backbone

Tags

FokI nuclease (ELD)

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pTRKH3-ermGFP

Plasmid

#27169

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5

Expression

Bacterial

Mutation

presence of an additional 183-bp region at N term…

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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Tailless VinculinTS

Plasmid

#26020

Depositor

Martin Schwartz

Article

Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

Insert

tailless vinculinTS

Expression

Mammalian

Available Since

Sept. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Hygro DEST (693-2)

Plasmid

#17291

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pYPet-His

Plasmid

#14031

Depositor

Patrick Daugherty

Article

Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

Insert

YPet

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 14, 2007

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER Short P2A

Plasmid

#164108

Purpose

Detect the short Mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Mt-ER Short P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_ORI vector

Plasmid

#99296

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pC001 - huLshC2C2-MBP for bacterial expression

Plasmid

#79150

Purpose

Expresses human codon-optimized LshC2c2 for purification in E. coli

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

C2c2

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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Pyronic/pcDNA3.1(-)

Plasmid

#51308

Purpose

Pyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.

Depositor

Luis Felipe Barros

Article

San Martin et al PLoS One. 2014 Jan 21;9(1):e85780. doi:

Insert

Pyronic

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomTagBFP2-Kan

Plasmid

#44899

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mTagBFP2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomTagBFP2

Expression

Yeast

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pTOM20-ddGFP-B

Plasmid

#40291

Depositor

Robert Campbell

Article

Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

Insert

TOMM20 (TOMM20 Human)

Tags

ddGFP-B

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pMJ841

Plasmid

#39318

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A) and Histidine 840 …

Promoter

T7

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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