163,606 results

1. v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

   Plasmid

   #207860

   Purpose

   AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
   

   Use

   AAV

   Tags

   Expression

   Mutation

   See Manuscript

   Promoter

   Cbh

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1-FLAG-Ku80

   Plasmid

   #46958

   Purpose

   Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   Ku80 (XRCC5 Human)
   

   Use

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-VSVG

   Plasmid

   #64084

   Purpose

   Expression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein gene

   Depositor

   Ian Wickersham

   Insert

   VSV glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. MOM603

   Plasmid

   #133242

   Purpose

   expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

   Depositor

   Shinsuke Niwa

   Article

   Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

   Insert

   kif1a (KIF1A Human)
   

   Use

   Tags

   StrepII tag and mScarlet

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. EphA2-YFP

   Plasmid

   #108852

   Purpose

   Encodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

   Depositor

   Kalina Hristova

   Article

   Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

   Insert

   EPHA2 (EPHA2 Human)
   

   Use

   Tags

   Labeled with eYFP on the C-Terminus via a 15 amin…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA

   Plasmid

   #200641

   Purpose

   Expresses miniTurboID-FKBPF36V-2xHA in mammalian cells.

   Depositor

   Fleur Ferguson, Justin English

   Article

   Tao et al Nat Commun. 2023 Dec 4;14(1):8016. doi:

   Insert

   miniTurboID-6xGGSG linker-FKBPF36V-2xHA
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWN_U6-B2M-miniGag-Cas9

   Plasmid

   #228958

   Purpose

   miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. EF1a-L274GMmMetRS-T2A-mCherry

   Plasmid

   #220800

   Purpose

   Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance

   Depositor

   Elizabeth Kirby

   Article

   Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18

   Insert

   Mars1 (Mars1 Mouse)
   

   Use

   Lentiviral

   Tags

   2xFLAG and T2A-mCherry

   Expression

   Mutation

   mutation in MetRS to change aa 274 from L to G

   Promoter

   EF1a

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAB151

   Plasmid

   #101666

   Purpose

   Opto-T7RNAP*(563-F1)

   Depositor

   Mustafa Khammash

   Article

   Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

   Insert

   Opto-T7RNAP*(563-F1)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD24-sfGFPx1

    Plasmid

    #51558

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli

    Depositor

    Francisco Malagon, Sankar Adhya

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-LK03

    Plasmid

    #206512

    Purpose

    AAV packaging vector containing AAV2 rep and LK03 cap and partial AAV2 p5 promoter

    Depositor

    Mark Kay

    Article

    Lisowski et al Nature. 2014 Feb 20;506(7488):382-6. doi

    Insert

    LK03 capsid
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    p5

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLQ1651-sgTelomere(F+E)

    Plasmid

    #51024

    Purpose

    Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

    Depositor

    Bo Huang, Stanley Qi

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    Optimized sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. ZS006:eGFP-KRAS

    Plasmid

    #186433

    Purpose

    eGFP-KRAS

    Depositor

    Adam Cohen

    Article

    Shi et al Cell. 2018 Dec 13;175(7):1769-1779.e13.

    Insert

    eGFP-KRAS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC

    Plasmid

    #221357

    Purpose

    Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC; to be used with Anti-DYKDDDDK [IPI-4E11/M1] light chain (Plasmid 221358) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa

    Plasmid

    #221358

    Purpose

    Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa; to be used with Anti-DYKDDDDK [IPI-4E11/M1] heavy chain (Plasmid 221357) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pOsTIR1w/oGFP

    Plasmid

    #102883

    Purpose

    Expresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Insert

    OsTIR1
    

    Use

    Tags

    3x myc

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT3EF1aH-myr-Akt

    Plasmid

    #179909

    Purpose

    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

    Depositor

    Xin Chen

    Article

    Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMU-NUCr

    Plasmid

    #61168

    Purpose

    Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    NLS-mCherry-GUS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. CRY2olig-mCherry

    Plasmid

    #60032

    Purpose

    Expresses a fusion of CRY2PHR E490G with mCherry

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

    Insert

    CRY2PHR E490G (CRY2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E490G

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAB213

    Plasmid

    #101677

    Purpose

    mCherry under T7 promoter control

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    mCherry under T7 promoter control
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pNPTS138 Cm

    Plasmid

    #41891

    Depositor

    Howard Steinman

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector with sacb

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. pTP1122

    Plasmid

    #104159

    Purpose

    Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
    

    Use

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. eIF2a 1

    Plasmid

    #21807

    Depositor

    David Ron

    Insert

    eIF2a (EIF2S1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA3.1-SARS-CoV-2-S (Wuhan)

    Plasmid

    #215812

    Purpose

    Expresses SARS-CoV-2 Spike protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

    Plasmid

    #154786

    Purpose

    Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE

    Depositor

    Robert Singer

    Article

    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

    Insert

    Adenosine deaminse (Adar Fly, Synthetic)
    

    Use

    Lentiviral

    Tags

    GFP, V5, and mCherry

    Expression

    Mammalian

    Mutation

    Synonymized serine to prevent autoediting of ADAR…

    Promoter

    UBC

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA-iLACCO1.2

    Plasmid

    #218823

    Purpose

    Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    iLACCO1.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pMVA2RBS035

    Plasmid

    #121051

    Purpose

    Expresses recombinant MVA pathway in Escherichia coli

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    mvaE
    mvaS
    mvaK1
    mvaK2
    mvaD
    idi
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO-MCS

    Plasmid

    #185594

    Purpose

    Control lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOpen-DpnIR

    Plasmid

    #165529

    Purpose

    Used to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    DpnI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. p6599 MSCV-IP N-HAonly FOSL1

    Plasmid

    #34897

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    FOSL1 (FOSL1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available Since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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