176,436 results
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Plasmid#141347Purpose3rd Generation lentiviral vector expressing the codon-optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M PaoDepositorInsertSARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
UseLentiviralTagsHAMutationresynthesized with human codon optimization nucle…PromoterCAGAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-T7-EGFP (BPK1098)
Plasmid#133962PurposeCMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcriptionDepositorInsertEGFP
ExpressionMammalianPromoterCMV and T7Available sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_PGK_antiCD19_synNotch_TetRVP64
Plasmid#79126PurposeLentiviral vector for constitutive expression of the anti-CD19 scFv synNotch TetRVP64 Receptor (PGK promoter)DepositorInsertantiCD19_synNotch_TetRVP64
UseLentiviralAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCIS GEM-CEPIA1er
Plasmid#58217PurposeRatiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorInsertGEM-CEPIA1er
TagsmycExpressionMammalianMutationGEM-GECO1 E298D F359W D400E E407DPromoterCAGAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hprtSAGFP
Plasmid#41594PurposeIn vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequencesDepositorInsertSA-GFP reporter
UseMouse Targeting; Dsb repair reporter substrateExpressionMammalianAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SlugMyc_pcDNA3
Plasmid#31698DepositorAvailable sinceOct. 7, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCVhygro-F-Ezh2
Plasmid#24926DepositorAvailable sinceJune 27, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWZL Blast GFP
Plasmid#12269DepositorPublicationInsertEGFP
UseRetroviralExpressionMammalianAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
STOP-eGFP-ROSA26TV
Plasmid#11739DepositorPublicationInsertSTOP-eGFP-ROSA26TV
UseCre/Lox; /stopAvailable sinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV5B-Flag-Smurf1 wt
Plasmid#11752DepositorPublicationAvailable sinceMay 12, 2006AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pBABE-hygro CDK4 R24C
Plasmid#11254DepositorPublicationAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-PE7 for IVT
Plasmid#214813PurposeTemplate for in vitro transcription of PE7DepositorInsertPE7
UseTemplate for in vitro transcriptionTagsSV40 bpNLS and c-Myc NLSPromoterT7 (inactivated)Available sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSETB/mStayGold
Plasmid#212017PurposemStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.DepositorTypeEmpty backboneTags6xHis, T7 tag, Xpress tag, mStayGoldExpressionBacterialPromoterT7Available sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
Plasmid#179105PurposepCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINTDepositorInsertpCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
UseCRISPRExpressionMammalianAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Fuw-dCas9-Tet1CD_IM
Plasmid#84479PurposeLentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)DepositorInsertdCas9-Tet1CD_IM
UseLentiviralExpressionMammalianAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby
Plasmid#71760PurposemGFP and Synaptophysin-mRuby expression from Cre-expressing neuronsDepositorHas serviceAAV1InsertsmGFP
Synaptophysin
UseAAV and Cre/LoxTagsPalmitoylation signal and mRubyAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-C1 F-tractin-EGFP
Plasmid#58473PurposeExpresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoterDepositorPublicationAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Proinsulin-NanoLuc in pLX304
Plasmid#62057PurposeLuminescent reporter of insulin secretionDepositorInsertProinsulin-NanoLuc (Ins2 Mouse, Synthetic)
UseLentiviral and LuciferaseExpressionMammalianMutationInserted NanoLuc into C-peptide of mouse Ins2 cod…PromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-DIO-mCherry
Plasmid#50459PurposeDouble floxed mCherry under the control of human synapsin promoterDepositorHas serviceAAV1, AAV2, AAV5, AAV8, AAV9, and AAV RetrogradeInsertmCherry
UseAAVTagsN/APromoterhuman Synapsin 1Available sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHD-DsRed
Plasmid#51434PurposeVector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.DepositorInsertLoxP-3xP3-DsRed-LoxP
Promoter3xP3Available sinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)
Plasmid#47868PurposeThis plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.DepositorPublicationInsertsNmCas9
U6pr-tracrRNA
UseCRISPRTagsHA and NLSExpressionMammalianPromoterEF1a and U6Available sinceSept. 30, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pINDUCER11 (miR-RUG)
Plasmid#44363PurposeInducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interestDepositorInsertPheS Gly294
UseLentiviralAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK-ATF3
Plasmid#26115DepositorAvailable sinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
eIF2a 3
Plasmid#21809DepositorAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
aMHC-mCherry-Rex-Blasticidin
Plasmid#21228DepositorPublicationInsertalpha myosin heavy chain promoter
UseLentiviralTagsmCherryAvailable sinceJune 30, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LRRK2-WT
Plasmid#17609DepositorAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAC-BA-lacZ
Plasmid#13423DepositorInsertBA
UseCre/LoxExpressionBacterialAvailable sinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti6-DEST PINK1-V5 WT
Plasmid#13320DepositorAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMKO.1 puro GFP shRNA
Plasmid#10675PurposeNegative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.DepositorInsertGFP shRNA
UseRNAi and RetroviralExpressionMammalianAvailable sinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBABE-FLAG-LKB1
Plasmid#8592DepositorAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by