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Showing: 2851 - 2900 of 18928 results

  1. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

    Plasmid
    #227245
    Purpose
    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell
    Depositor
    Jae Seong Lee
    Article
    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.
    Inserts
    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α and pTRE3G
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    227245-plasmid-map-sequence-id-450568

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  9. pUASTLOTattB_VHH-GFP4::CD8::mCherry

    Plasmid
    #163917
    Purpose
    Expression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancers
    Depositor
    Markus Affolter
    Article
    Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do
    Insert
    Fusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
    Use
    Cre/Lox
    Tags
    mCherry
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163917-plasmid-map-sequence-id-323453

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  15. pJS145

    Plasmid
    #179159
    Purpose
    Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.
    Depositor
    Iva Greenwald
    Article
    Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11
    Insert
    rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Flexon replacing 2nd intron of 2xNLS::GFP
    Promoter
    rps-27p
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    179159-plasmid-map-sequence-id-352801

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  21. pBV65

    Plasmid
    #108363
    Purpose
    A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.
    Depositor
    Scott Moye-Rowley
    Article
    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0
    Insert
    loxP-natMX6-CgMET3prom-cre-loxP
    Use
    Gene disruption marker for candida glabrata
    Tags
    Expression
    Mutation
    Promoter
    C. glabrata MET3
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    108363-plasmid-map-sequence-id-209915

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  23. pTC-ApoE-Tet-YAP

    Plasmid
    #85580
    Purpose
    Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (ApoE.HCR.hAAT promoter) in mouse liver
    Depositor
    Ursula Ehmer
    Article
    Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg
    Inserts
    CreER
    YAP
    Use
    Cre/Lox
    Tags
    Flag (2x)
    Expression
    Mammalian
    Mutation
    Promoter
    ApoE.HCR.hAAT and TRE
    Available since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85580-plasmid-map-sequence-id-169837

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  30. pCAG-lox-rox-STOP-rox-lynScarlet-lox-Lyn-NeonGreen

    Plasmid
    #175259
    Purpose
    dual plasmid labelling membrane target protein of different neuronal population with different flurophore
    Depositor
    Frank Bradke, Sebastián Dupraz
    Article
    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37
    Insert
    Lox-rox-STOP-rox-lyn-mScarlet-pA-lox
    Use
    Cre/Lox; Dre/rox
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    pCAG
    Available since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    175259-plasmid-map-sequence-id-375583

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  36. pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)

    Plasmid
    #129538
    Purpose
    modified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selection
    Depositor
    Arneet Saltzman
    Article
    Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1
    Type
    Empty backbone
    Use
    Cre/Lox; Mos1-mediated single copy insertion (mos…
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    129538-plasmid-map-sequence-id-258304

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  41. pMJB1-RPS22B-Cl

    Plasmid
    #160431
    Purpose
    Expresses "cre-less" yeast RPS22B tagged with mCherry under the GAL1 promoter
    Depositor
    Gary Stormo
    Article
    Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:
    Insert
    RPS22B (RPS22B Budding Yeast)
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Removed introns, shuffled codons to change nucleo…
    Promoter
    GAL1 and S. cerevisiae GAL1
    Available since
    Nov. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    160431-plasmid-map-sequence-id-317512

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  45. Double UP mNeon to mScarlet Rac1-V12

    Plasmid
    #125136
    Purpose
    Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-V12 (Constitutively Active).
    Depositor
    Erik Dent
    Article
    Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d
    Insert
    mNeon/mScarletP2A Rac1-V12
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Removed Not1 site in mScarlet. 12th AA in Rac1 ch…
    Promoter
    CAG
    Available since
    May 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    125136-plasmid-map-sequence-id-244760

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  50. Double UP mNeon to mScarlet Rac1-N17

    Plasmid
    #125137
    Purpose
    Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-N17 (Dominant Negative).
    Depositor
    Erik Dent
    Article
    Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d
    Insert
    mNeon/mScarletP2A Rac1-N17
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Removed Not1 site in mScarlet. 17th AA in Rac1 ch…
    Promoter
    CAG
    Available since
    May 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    125137-plasmid-map-sequence-id-244754

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Showing: 2851 - 2900 of 18928 results