We narrowed to 15,350 results for: genome
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Plasmid#169431PurposeDonor DNA for TKIT knockin of SEP to NR2B in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-NS
Plasmid#149546PurposeExpression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.DepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterU6Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluA2 TKIT donor
Plasmid#169421PurposeDonor DNA for TKIT knockin of SEP to GluA2 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKIKOlacZKm
Plasmid#46767PurposeE.coli genomic integration at lacZDepositorTypeEmpty backboneUseE.coli genomic integrationTagsExpressionMutationPromoterAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
PNCA-GFP
Plasmid#67789PurposeUse together with pCMV-intron and VSV-G to package MMLV reporter virusDepositorInsertMMLV-LTR-GFP
UseTagsExpressionMutationPromoterAvailable sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDHt/sk-CC
Plasmid#92127PurposeExpression of codon-optimized Cas9 with Pcbh1 promoter in Trichoderma reeseiDepositorInsertCas9
UseCRISPR; Trichoderma reesei expressionTagsExpressionMutationPromoterPcbh1Available sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAR-Ec318
Plasmid#130871PurposeA nuclear plasmid encoding the WT TP-DNAP1 for OrthoRepDepositorInsertTP-DNAP1
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluN1 TKIT donor
Plasmid#169427PurposeDonor DNA for TKIT knockin of SEP to NR1 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330S-4
Plasmid#58780PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141C
Plasmid#69292PurposeGateway entry vector; single gRNA under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTOX5
Plasmid#127450PurposeEncodes MqsR toxin and amilCP chromoprotein. CAM-RDepositorInsertsmqsR toxin
amilCP chromoprotein
UseTagsExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
His_CL7_2NLS_iCas12a-4NLS
Plasmid#222971PurposeBacterial expression of NLS-rich iCas12a constructDepositorInsertHis_CL7_2NLS_iCas12a-4NLS
UseCRISPRTagsCL7 and HisExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cas9m4
Plasmid#47316PurposeCas9 D10A+D839A+H840A+N863ADepositorInsertCas9m4
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRN10
Plasmid#84454Purposeshuttle plasmid for sarA P1-mKate expressionDepositorInsertmKate2
UseShuttle vector e.coli-s.aureus, expression of sgf…TagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
VKG1-gRNA-pX330
Plasmid#108671PurposeCleavage of VKG1 sequence by CRISPR/Cas9DepositorInsertgRNA for VKG1 sequence
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
LT2_12_GlyRNA
Plasmid#177710PurposePlasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)DepositorInsertspTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p201B Cas9
Plasmid#59177PurposeCas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
BAR
UseCRISPRTagsExpressionMutationPromoter2x35SAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVKG1-Puro
Plasmid#108670PurposePuromycin resistant cassette for knock-in by VIKING methodDepositorInsertPURO
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBA562
Plasmid#186237PurposepTet-RfxCas13d crRNA entry vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEVA13-GG
Plasmid#208002Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
UseTagsExpressionMutationPromoter14GAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B6/7deltaE3
Plasmid#175748PurposeA block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6deltaE3 and pAd5-B7.DepositorInsertAdenovirus 5 genomic region 25043-35938
UseAdenoviral and Synthetic BiologyTagsExpressionMutationAdenovirus sequences 27859-30803 replaced with Ba…PromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA
Plasmid#108549PurposeBacterial Target-AID vectorDepositorInsertdCas9-PmCDA1
UseTagsFlagExpressionBacterialMutationD10A and H840A for SpCas9Promoterlambda ORAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x3
Plasmid#58767PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP E17TAG
Plasmid#68295PurposeE17TAG GFP reporterDepositorInsertE17TAG GFP
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-PCP-3XNeon-nls
Plasmid#75386PurposePCP-3XNeon-nlsDepositorInsertPCP
UseLentiviralTags3XmNeonGreenExpressionMammalianMutationPromoterEFSAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas9m4-VP64
Plasmid#47319PurposeCas9m4 ActivatorDepositorInsertCas9m4-VP64
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKIKOrbsARCm
Plasmid#46765PurposeE.coli genomic integration rbsAR locusDepositorTypeEmpty backboneUseE.coli genomic integrationTagsExpressionMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTH2
Plasmid#84452Purposeshuttle plasmid for sarA P1-CFP expressionDepositorInsertCFP Cerulean
UseShuttle vector e.coli-s.aureus, expression of sgf…TagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330S-5
Plasmid#58781PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRN12
Plasmid#84456Purposeshuttle plasmid for sarA P1-mAmetrine expressionDepositorInsertmAmetrine
UseShuttle vector e.coli-s.aureus, expression of sgf…TagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1-2XboxB
Plasmid#75391PurposesgRNA1-2XboxBDepositorInsertsgRNA1-2XboxB
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ146
Plasmid#69298PurposeGolden Gate recipient and Gateway entry vector; assembly of 6 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKIKOrbsARKm
Plasmid#46768PurposeE.coli genomic integration rbsAR locusDepositorTypeEmpty backboneUseE.coli genomic integrationTagsExpressionMutationPromoterAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination
Plasmid#40132PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).DepositorTypeEmpty backboneUseTagsFokI nuclease (ELD)ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination
Plasmid#40131PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).DepositorTypeEmpty backboneUseTagsFokI nuclease (KKR)ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly
Plasmid#71499PurposeflySTARR-seq screening vector with DSCP core promoter.DepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectMutationPromoterAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLSL
Plasmid#51493PurposeT-DNA destination plasmid. Contains the bean yellow dwarf virus cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. Contains attR1 and attR2 sites between the LIR and SIR sequence.DepositorTypeEmpty backboneUsePlant t-dna plasmidTagsExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-MCP-3XBFPnls
Plasmid#75383PurposeMCP-3XBFPnlsDepositorInsertMCP
UseLentiviralTags3XBFPExpressionMammalianMutationPromoterCMV-TOAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
p177bp-LacOx162-Hyg
Plasmid#210215PurposeIntegrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.DepositorInsert162xLacO repeats derived from: PMID: 12864855.
UseTagsExpressionMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-Destination
Plasmid#37184PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.DepositorTypeEmpty backboneUseTagsFokI nucleaseExpressionMammalianMutationPromoterCAGAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTarget12f1
Plasmid#171183PurposeConstitutive expression of sgRNA without donor DNADepositorInsertsgRNA scaffold in the CRISPR/Cas12f1 system
UseCRISPRTagsExpressionBacterialMutationPromoterJ23119(SpeI) promoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKDR-EF1a
Plasmid#183567PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated EF1a promoter)DepositorInsertUnattenuated
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV-EF1s HA.NLS.Sce(opt)
Plasmid#31479DepositorInsertSFFV/Ef1s HA NLS I-SceI
UseLentiviralTagsHA and NLSExpressionMutation"opt" indicates the coding sequence has…PromoterAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHFS:SEP-rGluA1 TKIT donor
Plasmid#169438PurposeDonor DNA for TKIT knockin of SEP to GluA1 in ratDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRGE31
Plasmid#50929PurposeExpressing sgRNA and Cas9 in plants. The sgRNA was controlled by rice snoRNA U3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRice snoRNA U3 and dual 35S promoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
PX458_RAD21_1
Plasmid#64057PurposeExpresses gRNA against human RAD21 along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDD285
Plasmid#66826PurposemKate2^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmKate2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized mKate2ExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pREP
Plasmid#51491PurposepMDC32 with Rep/RepA coding sequence from the mild bean yellow dwarf virusDepositorInsertRep/RepA
UsePlant expression t-dnaTagsExpressionMutationnonePromoter2x35SAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBTK1024 (pDS-Def1)
Plasmid#183125PurposeExpresses double-stranded RNA targeting the Def1 gene from Apis melliferaDepositorInsertFragment of Def1 gene
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330S-6
Plasmid#58782PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only