We narrowed to 15,350 results for: genome
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Plasmid#66590PurposeLox-STOP-Lox reporterDepositorInsertLSL-Tomato
UseTagsExpressionMutationPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x6
Plasmid#58770PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x5
Plasmid#58769PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMLS485
Plasmid#188890PurposeWorm Cas9 expression vector, Peft-3 promoterDepositorInsertPeft-3::Cas9
UseTagsExpressionWormMutationC. elegans codom optimization and intron additionPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR1056
Plasmid#71824PurposeExpress Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-beta2 TKIT donor
Plasmid#169436PurposeDonor DNA for TKIT knockin of SEP to Gabrb2 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
BSP611
Plasmid#122256Purposeexpression vector for mtagRFP-T under EFT3 promoterDepositorInsertmtagRFP-T
UseTagsExpressionWormMutationPromoterEFT3Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTaU6-esgRNA
Plasmid#115630Purposeexpression esgRNA in wheat protoplastsDepositorInsertTaU6p-esgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
SP6-sgRNA-scaffold
Plasmid#47912PurposeScaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injectionDepositorTypeEmpty backboneUseCRISPRTagssgRNA 3' endExpressionWormMutationPromoterSP6Available sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cip-pAct1-Mnase-SAT1
Plasmid#162557PurposeFor the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1DepositorInsert3FLAG-Mnase-SV40
UseTagsExpressionYeastMutationPromoterActineAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX458_CEBPB_1
Plasmid#64036PurposeExpresses gRNA against human CEBPB along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x7
Plasmid#58771PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
PX458_CEBPB_2
Plasmid#64047PurposeExpresses gRNA against human CEBPB along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC1876
Plasmid#158722PurposeHelper plasmid pYC1640 for C-terminal tagging with degradation tag ssrADepositorInsertssrA
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ148
Plasmid#69300PurposeGolden Gate recipient and Gateway entry vector; assembly of 8 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAEF5
Plasmid#136305PurposePlasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin FleissDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDCC5
Plasmid#59984PurposeBi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassetteDepositorInsertCas9-D10A
UseCRISPRTagsHAExpressionInsectMutationD10APromoterhsp70BbAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJP118
Plasmid#176494PurposeExpresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoterDepositorInsertLifeact peptide
UseCapsaspora owczarzaki expressionTagsExpressionMutationPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP S2TAG
Plasmid#68296PurposeS2TAG GFP reporterDepositorInsertGFP S2TAG
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDonorTp
Plasmid#211788PurposepDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpRDepositorInsertdhfr
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-nCas9n-A71
Plasmid#125644Purposemicro-injection of poly-adenylated nCas9n RNADepositorInsertnCas9n
UseRna in vitro transcription vectorTagsExpressionMutationPromoterT3Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-Fn-crRNA
Plasmid#78958PurposeCloning vector for expression of FnCpf1 crRNA. It contains BsmB1 site for cloning.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEVA43-GG
Plasmid#208014Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
UseTagsExpressionMutationPromoter14GAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGb_PMLlxP_EGFP
Plasmid#140283Purposea targeting plasmid for EGFP-PMLDepositorInsertEGFP
UseMouse TargetingTagsExpressionMutationPromoterendogenousAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anep8_Cas9
Plasmid#117169PurposeExpressed Cas9 in Aspergillus with LIC tags for easy gRNA cloningDepositorTypeEmpty backboneUseCRISPR and Synthetic Biology; Filamentous fungi e…TagsExpressionMutationPromoterPkiAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluA3 TKIT donor
Plasmid#169425PurposeDonor DNA for TKIT knockin of SEP to GluA3 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
EZH2_N_FS_v2_Donor
Plasmid#79901PurposeN-terminal tagging of human EZH2 with 3xFLAG_Twin_StrepDepositorTypeEmpty backboneUseCRISPR and TALENTags3xFLAG_Twin_StrepExpressionMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAK005
Plasmid#128218Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 5DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 5
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK003
Plasmid#128216Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 3DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 3
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK004
Plasmid#128217Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 4DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 4
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK006
Plasmid#128219Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 6DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 6
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK008
Plasmid#128225Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 2DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 2
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK009
Plasmid#128226Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 3DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 3
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet26
Plasmid#107448PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet26 (met26 Fission Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet16
Plasmid#107452PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet16 (met16 Fission Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAK002
Plasmid#128215Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 2DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 2
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1-PP7-boxB
Plasmid#75393PurposesgRNA1-PP7-boxBDepositorInsertsgRNA1-2XPP7-boxB
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-SAT1
Plasmid#162465PurposepFA-MNase-CaURA3 was double digested to remove the URA3 auxotrophy marker. The SAT1 marker was amplified from pFA-SAT1 and cloned into the AscI-SacI-digested pFA-MNaseDepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
BSP606
Plasmid#122251Purposeexpression vector for mECitrine under EFT3 promoterDepositorInsertmECitrine
UseTagsExpressionWormMutationPromoterEFT3Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
iGFP
Plasmid#66589Purposeinverted GFP reporterDepositorInsertGFP
UseTagsExpressionMutationPromoterCMVAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEVA231-CRISPR
Plasmid#138712PurposePlasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR ArrayDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJY3919
Plasmid#113332PurposeHermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoterDepositorInsertsbeta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICSL50005
Plasmid#117536PurposeLevel0 Golden Gate part: c-terminal tag, YFPDepositorInsertc-term tag YFP
UseSynthetic BiologyTagsExpressionMutationG65S, L68V, A72S, Y203T, L231HPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP S2TAG/E17TAG
Plasmid#68298PurposeS2TAG/E17TAG GFP reporterDepositorInsertGFP S2TAG/E17TAG
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYZ714
Plasmid#132955PurposecrRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombeDepositorInsertdFnCas12a
UseCRISPR and Synthetic BiologyTagsSV40 NLS and nucleoplasmin NLSExpressionMutationPromoteradh1Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSfi-HspLacZ
Plasmid#33351DepositorInsertHsp68 promoter (Hspa1a Mouse)
UseMouse TargetingTagsLacZExpressionMammalianMutationPromoterHsp68 (Hspa1a)Available sinceJan. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJYScr
Plasmid#132719PurposePlasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistanceDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_PA-Cre-Y324F
Plasmid#122961PurposeCatalytically inactive mutant of PA-CreDepositorInsertpcDNA3.1_PA-Cre-Y324F
UseCre/LoxTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-Blast-mU6
Plasmid#206806PurposepLentiCRISPRv2 variant with mU6 promoter driving the sgRNA, and a Blasticidin resistance cassetteDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Hulk-ubi-lacZ-to-Green
Plasmid#108641Purposeubiquitous promoter followed by a lacZ to GFP lineage tracing line. Cryst:venusGFPDepositorInsertubi-lacZ-to-GFP
UseTagsExpressionMutationPromoterAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only