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Addgene

We narrowed to 15,350 results for: genome

Showing: 3051 - 3100 of 15350 results
  1. SP6-sgRNA-scaffold

    Plasmid
    #47912
    Purpose
    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    sgRNA 3' end
    Expression
    Worm
    Mutation
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
  2. pAEF5

    Plasmid
    #136305
    Purpose
    Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pJP118

    Plasmid
    #176494
    Purpose
    Expresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter
    Depositor
    Insert
    Lifeact peptide
    Use
    Capsaspora owczarzaki expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pFA-MNase-SAT1

    Plasmid
    #162465
    Purpose
    pFA-MNase-CaURA3 was double digested to remove the URA3 auxotrophy marker. The SAT1 marker was amplified from pFA-SAT1 and cloned into the AscI-SacI-digested pFA-MNase
    Depositor
    Insert
    3FLAG-Mnase
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pYZ714

    Plasmid
    #132955
    Purpose
    crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombe
    Depositor
    Insert
    dFnCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS and nucleoplasmin NLS
    Expression
    Mutation
    Promoter
    adh1
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pJYScr

    Plasmid
    #132719
    Purpose
    Plasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistance
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 3051 - 3100 of 15350 results