161,641 results
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Plasmid#72322PurposeBacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.DepositorInsertpET28a-SnoopCatcher
UseTagsHis6 tagExpressionBacterialMutationchanged G842T and D848G of S. pneumoniae adhesin …PromoterT7Available sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag beta-1-adrenergic-receptor
Plasmid#14698DepositorInsertB1AR (ADRB1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptSKII+ U6-sgRNA(F+E) empty
Plasmid#74707PurposeEncodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequenceDepositorInsertU6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV_AncBE4max_P2A_GFP
Plasmid#112100PurposeC:G-to-T:A base editingDepositorInsertAncBE4max_P2A_GFP
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetO-hNGN2-Puro
Plasmid#79049PurposeExpresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.DepositorInserthNGN2-P2A-PuroR (NEUROG2 Human)
UseLentiviralTagsExpressionMutationPromoterTetOAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a-BirA
Plasmid#20857DepositorInsertBirA
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA
Plasmid#71409PurposeThere is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBIDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
MBP-Benzonase
Plasmid#218192PurposeBacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001DepositorInsertnucA
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceApril 24, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSpliceExpress
Plasmid#32485DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP(C3)-Nup153
Plasmid#64268PurposeGFP-tagged mammalian expression of Nup153DepositorInsertNuP153 (NUP153 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pLKO5.sgRNA.EFS.GFP
Plasmid#57822Purpose3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
eGFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin
Plasmid#187952PurposeFKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertFKBP12_F36V-CasRx-tagBFP
UseCRISPR and Synthetic BiologyTagsGGS linker, HA-tag, and NLSExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NLS-iPASnFR-H-mScarlet3-3xFLAG
Plasmid#225245PurposeMammalian expression of iPASnFR-H in the nucleusDepositorInsertiPASnFR-H
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-pAG-Hia5
Plasmid#174373PurposePlasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)DepositorInsertprotein AG - hia5
UseTags6xHis and Protein A/GExpressionBacterialMutationPromoterAvailable sinceSept. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM5-WT PHGDH
Plasmid#83901Purposestable overexpressionDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCI HA NEDD4
Plasmid#27002DepositorInsertNEDD4 (NEDD4 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core
Plasmid#61357Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICH41331::pU6cm-pegR::EURb7
Plasmid#227695PurposeGolden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).DepositorInsertAltered epegRNA backbone with cloning sites for gRNA and RTT.
UseCRISPRTagsExpressionPlantMutationPromoterU6 compositeAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Rab11a-7
Plasmid#55124PurposeLocalization: Ras GTPase, Excitation: 587, Emission: 610DepositorInsertRab11a (RAB11A Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
p6XHis-T7(P266L)
Plasmid#174866PurposeExpresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.DepositorInsertT7 RNA polymerase
UseTags6XHis-tagExpressionBacterialMutationP266LPromoterlac promoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO SV40 small + Large T (w612-1)
Plasmid#22298DepositorInsertSV40 small + Large T antigen
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
SF-SON IRES Puro
Plasmid#214402PurposeExpresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)DepositorUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
XLone-GFP
Plasmid#96930PurposeTunable and temporal expression control of GFPDepositorInsertGreen Fluorescent Protein
UseTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Hygro (656-4)
Plasmid#17446Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, HygroDepositorHas ServiceLentiviral PrepInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
gag-PE
Plasmid#211371PurposePackaging plasmid for v3 PE-eVLP productionDepositorInsertMMLVgag-PE
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry
Plasmid#114472PurposemCherry ControlDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertmCherry
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT3-N90-beta-catenin
Plasmid#31785DepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsMycExpressionMammalianMutationDeletion of aa 1-90- Constitutitvely ActivePromoterEF1alphaAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pXR002: EF1a-dCasRx-2A-EGFP
Plasmid#109050PurposeCatalytically inactive dCasRx with 2A-EGFPDepositorInsertdCasRx
UseCRISPR and LentiviralTags2A-eGFP, HA, and NLSExpressionMammalianMutationR239A/H244A/R858A/H863APromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003-sfGFP
Plasmid#133449PurposeExpresses SpyCatcher003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyCatcher003-sfGFP
UseTagsHis6 and TEV siteExpressionBacterialMutationPromoterT5Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-gp32-H6
Plasmid#163912PurposeExpresses T4 gp32 for bacterial expression and affinity purificationDepositorInsertgp32
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP12558 - pAAV-AiE0390m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2558)
Plasmid#208141PurposeAiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytesInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterBeta Globin minimal promoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pED9x (dCas9-KRAB-mCherry)
Plasmid#163956PurposeLentiviral expression plasmid of sgRNA with mCherryDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS TIR-2 sfGFP
Plasmid#154464PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7pol
Plasmid#59926PurposeExpression vector for T7 RNA polymerase geneDepositorInsertT7 RNA polymerase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV enAsCas12f-HKRA-sgRNA_v7
Plasmid#208357PurposeExpression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cellsDepositorInsertenAsCas12f-HKRA
UseTagsExpressionMammalianMutationI123H/D195K/D208R/V232APromoterCMVAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSIRV-NFAT-eGFP
Plasmid#118031PurposeFluorescent reporter for NFAT activationDepositorInserteGFP
UseRetroviralTagsExpressionMutationPromoterminimal promoter with NFAT binding siteAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.0-TERT WT
Plasmid#84924Purposeluciferase reporter for TERT promoterDepositorInsertTERT promoter (TERT Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterTERTAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
PA-GFP-α-Tubulin
Plasmid#213900PurposeImaging of Tubulin using Photoactivatable GFPDepositorInsertPA-GFP-α-Tubulin
UseTagsPhotoactivatable GFPExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only