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175,573 results

Showing: 3151 - 3200 of 175573 results

  1. pCAG-T7-TALEN(Sangamo)-Destination

    Plasmid
    #37184
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Tags
    FokI nuclease
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    July 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    37184-plasmid-map-sequence-id-46727

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  15. pRK5-myc-Rac1-T17N

    Plasmid
    #12984
    Depositor
    Gary Bokoch
    Insert
    Rac1 DN (RAC1 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    T17N Dominant negative
    Available Since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
    12984-plasmid-map-sequence-id-223318

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  19. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid
    #140002
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140002-plasmid-map-sequence-id-275516

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  21. pLenti6.2_miRFP670

    Plasmid
    #113726
    Purpose
    Expresses the fluorescent protein miRFP670 in a lentiviral backbone
    Depositor
    Vanessa LaPointe
    Insert
    miRFP670
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    113726-plasmid-map-sequence-id-218247

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  23. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid
    #90085
    Purpose
    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Article
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90085-plasmid-map-sequence-id-182691

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  27. EBFP2-H2B-6

    Plasmid
    #55243
    Purpose
    Localization: Nucleus/Histones, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55243-plasmid-map-sequence-id-159436

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  30. mApple-LAMP1-phLuorin-N-8

    Plasmid
    #54918
    Purpose
    Localization: Lysosome Membrane, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    LAMP1 phLuorin
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Lysosomes-20 double labeled with phLuorin
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    54918-plasmid-map-sequence-id-372787

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  32. AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato

    Plasmid
    #51082
    Purpose
    Cre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV1
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Promoter
    hEF1a
    Available Since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51082-plasmid-map-sequence-id-175187

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Showing: 3151 - 3200 of 175573 results