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165,688 results

Showing: 3151 - 3200 of 165688 results

  6. pBFC0984

    Plasmid
    #231992
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with 2xBsaI spacer
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and pTet
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231992-plasmid-map-sequence-id-467618

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  12. TRUPATH Triple Gaq

    Plasmid
    #196058
    Purpose
    Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaQ-RLuc8 (GNAQ Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196058-plasmid-map-sequence-id-385868

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  16. pENN.AAV.cTNT.PI.eGFP.WPRE.rBG

    Plasmid
    #105543
    Purpose
    AAV expression of EGFP from cTNT promoter
    Depositor
    James M. Wilson
    Has Service
    AAV9
    Insert
    cTNT-PI-EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    cTNT
    Available Since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105543-plasmid-map-sequence-id-202437

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  25. pRRLSIN.cPPT.PGK-GFP.WPRE

    Plasmid
    #12252
    Purpose
    3rd generation lentiviral backbone
    Depositor
    Didier Trono
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    March 27, 2007
    Availability
    Academic Institutions and Nonprofits only
    12252-plasmid-map-sequence-id-444115

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  28. pRGEB32

    Plasmid
    #63142
    Purpose
    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.
    Depositor
    Yinong Yang
    Article
    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    Rice snoRNA U3 promoter for gRNA/PTG expression a…
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    63142-plasmid-map-sequence-id-339931

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  33. pLenti-CMV-eGFP-hTau P301L-WPRE

    Plasmid
    #233043
    Purpose
    To Express a GFP human Tau P301L fusion protein from a CMV promoter
    Depositor
    Jonathan Ploski
    Insert
    eGFP-hTau P301L fusion (MAPT Human, Synthetic)
    Use
    Lentiviral
    Tags
    EGFP
    Available Since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233043-plasmid-map-sequence-id-463782

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  38. GLP1R-DuET

    Plasmid
    #213253
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GLP1R (GLP1R Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213253-plasmid-map-sequence-id-430803

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  39. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid
    #208942
    Purpose
    The CE1 construct, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208942-plasmid-map-sequence-id-415667

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  44. pHrD-IRES-Fluc

    Plasmid
    #194250
    Purpose
    Human RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!
    Depositor
    Samson Jacob
    Article
    McCool et al RNA. 2022 Nov 2. pii: rna.079149.122. do
    Insert
    Firefly luciferase
    Use
    Luciferase
    Promoter
    human rDNA promoter and beginning of 5' ETS …
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    194250-plasmid-map-sequence-id-391338

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Showing: 3151 - 3200 of 165688 results