We narrowed to 18,353 results for: Tec

1. pCY 3090-07

   Plasmid

   #36232

   Depositor

   Anne Robinson

   Article

   Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

   Insert

   Streptoalloteichus hindustanus bleomycin (Sh ble)
   

   Use

   Yeast cassette for pcr and homologous recombinati…

   Tags

   yEpolylinker-mCherry-Zeocin

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 6xHis-MBP-huHkCas12a

   Plasmid

   #174683

   Purpose

   Expresses 6xHis-MBP tagged HkCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huHkCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBT138b-proA

   Plasmid

   #138514

   Purpose

   P3g

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   T7-RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Cx55.5:rasGFP

   Plasmid

   #105939

   Purpose

   in vitro transcription

   Depositor

   Caren Norden

   Article

   Weber et al Cell Rep. 2014 Apr 24;7(2):386-97. doi:

   Insert

   Ras
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pASKt15C+SrtA

   Plasmid

   #64983

   Purpose

   Expresses the wild type of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

   Insert

   sortase A
   

   Use

   Tags

   His6 tag and S tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59

   Promoter

   tetracycline promoter/operator

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX330A_dCas9-1x4

   Plasmid

   #63598

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. tdEos-Talin-N-22

   Plasmid

   #57673

   Purpose

   Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

   Insert

   Talin
   

   Use

   Tags

   tdEos

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pDM_noro_S2_lacZA

   Plasmid

   #118816

   Purpose

   T7 RNAP-driven expression of norovirus sense orientation toehold switches (S2) with a lacZ alpha subunit reporter.

   Depositor

   Alexander Green

   Article

   Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

   Insert

   Norovirus toehold switch S2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pVH234-Tier1-PmPGK1-tTA

   Plasmid

   #169574

   Purpose

   Tier-1 vector encoding PmPGK1-driven tTA expression (PmPGK1-tTA-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled transactivator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BCJJ344

    Plasmid

    #117503

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. YSBE 596 : pNAT_mCherry

    Plasmid

    #92088

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, NAt selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    mCherry
    

    Use

    tagging proteins in cryptococcus neoforman

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GWF135

    Plasmid

    #133614

    Purpose

    ANR-ANR-BFP-CAAX

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    ANR-ANR-BFP-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTMS1

    Plasmid

    #87799

    Purpose

    For constructing MBP-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.

    Depositor

    Yu-Zhu Zhang

    Article

    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

    Type

    Empty backbone

    Use

    Tags

    6xHis and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pATD

    Plasmid

    #141012

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    HSV-TK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJ291_WT_med

    Plasmid

    #128856

    Purpose

    Medium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. tdEos-Talin-18

    Plasmid

    #57672

    Purpose

    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Talin
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pGEX-4T-1-p19-T7

    Plasmid

    #46306

    Depositor

    Judy Lieberman

    Article

    Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

    Inserts

    p19
    DNA-1 site
    linker
    DNA-2 site
    

    Use

    Pro-sirna

    Tags

    6XHis and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pCellFree_G03 USF2

    Plasmid

    #67069

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    USF2 (USF2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pDM_noro_S6_lacZA

    Plasmid

    #118817

    Purpose

    T7 RNAP-driven expression of norovirus sense orientation toehold switches (S6) with a lacZ alpha subunit reporter.

    Depositor

    Alexander Green

    Article

    Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

    Insert

    Norovirus toehold switch S6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFA6a-zeoCV

    Plasmid

    #73890

    Purpose

    fission yeast drug selection marker

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Insert

    zeoCV
    

    Use

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCellFree_G03 TNF

    Plasmid

    #67062

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TNF (TNF Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

22. tdEos-Paxillin-22

    Plasmid

    #57653

    Purpose

    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Paxillin
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

23. pCellFree_G03 VPS29

    Plasmid

    #67052

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    VPS29 (VPS29 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pLpC-L-IFP-F[1] (mammalian)

    Plasmid

    #52901

    Purpose

    Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    L-IFP-F[1]
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Unknown

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pLpC-L-IFP-F[2] (mammalian)

    Plasmid

    #52902

    Purpose

    Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    L-IFP-F[2]
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Unknown

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pJC175e-DddI

    Plasmid

    #179693

    Purpose

    Mutagenesis plasmid with constitutive bacterial expression of DddI immunity protein

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [SD8] gIII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRS416 Gal-RNQ1-YFP

    Plasmid

    #18685

    Depositor

    Susan Lindquist

    Article

    Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

    Insert

    Rnq1 (RNQ1 Budding Yeast)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

28. pCellFree_G03 NFX1

    Plasmid

    #67131

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    NFX1 (NFX1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pMS22H

    Plasmid

    #133904

    Purpose

    Expression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTK101

    Plasmid

    #59561

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) mKate-G8-L7Ae-PEST 4xFF4

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) mKate-G8-L7Ae-PEST 4xFF4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GGE020

    Plasmid

    #120777

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position1-3. When using the GG platform for expressing only one gene (T1-3_Lip2)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    T1-3_Lip2
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCfB4796

    Plasmid

    #106131

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBT100.110

    Plasmid

    #122602

    Purpose

    clone CDA1 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    CDA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUC19/N-eCFP

    Plasmid

    #183155

    Purpose

    Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eCFP

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRSET FLIPE-10u

    Plasmid

    #13538

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207R
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    A207R in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. YSBE 559 : pNAT_6xHA

    Plasmid

    #92087

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, NAT selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    6xHA
    

    Use

    tagging proteins in cryptococcus neoforman

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Id2-Halo donor

    Plasmid

    #113116

    Purpose

    Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Id2 donor region including homology arms and HaloTag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBW829_pCAG-pExpr-LUT

    Plasmid

    #87554

    Purpose

    Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    EGFP
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAG-TetR-Rad18UBD

    Plasmid

    #119010

    Purpose

    Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    TetR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFLIP38

    Plasmid

    #65616

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-edCit-attR1 and attR2-edCer-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pHCKan-yibDp-GST

    Plasmid

    #134592

    Purpose

    Low Phosphate inducible GST

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    Glutathione S-transferase
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. GGE252

    Plasmid

    #120791

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsDown_GSY-NotI
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. GGE253

    Plasmid

    #120790

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsUp_GSY-NotI
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. YIp211-P30-His-POL30-3'

    Plasmid

    #99546

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA); URA3 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    POL30

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pFLIP43

    Plasmid

    #65618

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-edAFPt9-attR1 and attR2-edCer-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. 7xNLS-pMJ915v2-sfGFP

    Plasmid

    #88922

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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47. pUC19/mlcR25-mEGFP

    Plasmid

    #154295

    Purpose

    Fluorescent reporter for conony screening without an inducer (e.g. IPTG); TED module (Plac-lacO-mlcR25-mEGFP)

    Depositor

    Shigehiko Yumura

    Article

    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

    Insert

    25-bp fragment of the 3′ end of mlcR (mlcR25); mEGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. JME4393

    Plasmid

    #129657

    Purpose

    LYS5ex_CrisprCas9-yl_RFP: CAS9 vector with LYS5ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCfB4132

    Plasmid

    #106127

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pRSET FLIPE-1m

    Plasmid

    #13540

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    A207W in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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