165,688 results

1. pAAVone-AAV9-CMV-eGFP

   Plasmid

   #230928

   Purpose

   pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system.

   Depositor

   Qizhao Wang

   Article

   Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

   Insert

   eGFP
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.Syn.GCaMP6f.WPRE.SV40

   Plasmid

   #100837

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

   Promoter

   Synapsin

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMBP-LbCas12a

   Plasmid

   #113431

   Purpose

   Expresses 10xHis-MBP fused to LbCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Has Service

   Cloning Grade DNA

   Insert

   LbCas12a
   

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. murine TCR OTI-2A.pMIG II

   Plasmid

   #52111

   Purpose

   retroviral expression of both TCRalpha and TCRbeta using a single vector

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Inserts

   T cell receptor alpha chain (Tcra Mouse)
   T cell receptor beta chain (Tcrb Mouse)
   

   Use

   Retroviral

   Tags

   T2A (from Thosea asigna virus)

   Expression

   Mammalian

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 HA-Sumo2 WT

   Plasmid

   #48967

   Purpose

   Expresses HA SUMO2 Wild Type in mammalian cells

   Depositor

   Guy Salvesen

   Article

   Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

   Insert

   SUMO2 (SUMO2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-alphasynuclein-A53T

   Plasmid

   #40823

   Depositor

   David Rubinsztein

   Article

   Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

   Insert

   SNCA (SNCA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   A53T (aggregates more rapidly than the wild-type …

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas

   Plasmid

   #62225

   Purpose

   Constitutive expression of cas9 and inducible expression of lambda RED and sgR

   Depositor

   Sheng Yang

   Article

   Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

   Has Service

   Cloning Grade DNA

   Insert

   cas9
   

   Use

   CRISPR; Lambda red recombinase

   Expression

   Bacterial

   Promoter

   native cas9 promoter

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. EZ-Tet-pLKO-Blast

   Plasmid

   #85973

   Purpose

   Tet/Dox inducible shRNA lentivirus with blasticidin selection

   Depositor

   Cindy Miranti

   Article

   Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Promoter

   H1 / TetO

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Alpha-Synuclein-His

   Plasmid

   #186802

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   Alpha-Synuclein-His (SNCA Human)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYC-N

    Plasmid

    #207113

    Purpose

    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

    Depositor

    Yifan Cheng

    Article

    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. H2B-mCherry

    Plasmid

    #20972

    Purpose

    H2B fused to mCherry for chromatin visualization

    Depositor

    Robert Benezra

    Article

    Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

    Insert

    Histone cluster 1, H2bb (H2BC3 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    May 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-CMV-Blast-PIP-FUCCI

    Plasmid

    #138715

    Purpose

    Lentiviral expression of the cell cycle marker PIP-FUCCI

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-FUCCI
    

    Use

    Lentiviral

    Tags

    mCherry and mVenus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWM_12x601_30bpLinker

    Plasmid

    #157789

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_30bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pemiRFP670-tubulin

    Plasmid

    #136573

    Purpose

    Human alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    emiRFP670-tubulin
    

    Tags

    emiRFP670

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. px330-Pten-2

    Plasmid

    #162552

    Purpose

    sgRNA targeting Pten

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Pten (Pten Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-CMV-NES-jRGECO1a

    Plasmid

    #61563

    Purpose

    Red fluorescent calcium sensor protein

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    NES-jRGECO1a
    

    Tags

    nuclear export signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV

    Plasmid

    #24828

    Depositor

    Lin He

    Article

    Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. mini-pHelper-1.0

    Plasmid

    #230933

    Purpose

    The mini version (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production

    Depositor

    Qizhao Wang

    Article

    Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

    Type

    Empty backbone

    Use

    AAV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. qTAG-AAVS1-Ef1a-Blast

    Plasmid

    #227267

    Purpose

    Empty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-2xFLAG-SREBP-1c

    Plasmid

    #26802

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    SREBP-1c (SREBF1 Human)
    

    Tags

    2XFLAG

    Expression

    Mammalian

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLX304 CMV HA-HaloTag-FRB-sTurboID (C)

    Plasmid

    #153003

    Purpose

    Expresses low-affinity split-TurboID C-terminal fragment in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID (C) (G74)
    

    Use

    Lentiviral

    Tags

    FRB, HA, and HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRK5 alpha6

    Plasmid

    #16036

    Depositor

    Filippo Giancotti

    Article

    Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

    Insert

    alpha6 (ITGA6 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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23. GFP-C1-PLCdelta-PH

    Plasmid

    #21179

    Purpose

    encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

    Depositor

    Tobias Meyer

    Article

    Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

    Insert

    PLCdelta (PLCD1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PH domain of human PLC delta, amino acids Met1–Il…

    Available Since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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24. pHR-mCh-Cry2WT

    Plasmid

    #101221

    Purpose

    Blue-light dependent optogenetic protein Cry2

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    Cry2PHR
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid

    #73797

    Purpose

    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLKO.1 - TRC control

    Plasmid

    #10879

    Purpose

    Negative control shRNA vector containing non-hairpin insert.

    Depositor

    David Root

    Article

    Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

    Insert

    non-hairpin 18bp
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Mfn2-YFP

    Plasmid

    #28010

    Depositor

    Richard Youle

    Article

    Karbowski et al J Cell Biol. 2002 Dec 23. 159(6):931-8.

    Insert

    mitofusin 2 (MFN2 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    silent S508S

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLEX_305-N-dTAG

    Plasmid

    #91797

    Purpose

    Lentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG system

    Depositor

    Behnam Nabet, James Bradner

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FKBP F36V

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBS-EF1afull-H2B-mCherry-P2A-Fluc

    Plasmid

    #207253

    Purpose

    Lentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoter

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    H2B-mCherry-P2A-Fluc
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1alpha_full

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET MBP GFP LIC cloning vector (MBP-msfGFP)

    Plasmid

    #29750

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, AviTag (biotin), MBP, SiriusGFP, and TEV c…

    Expression

    Bacterial

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. T5-pAc5.1 EGFP-EcoRV

    Plasmid

    #206481

    Purpose

    Schneider cell imaging

    Depositor

    Anne Ephrussi

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Type

    Empty backbone

    Tags

    EGFP

    Expression

    Insect

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pWPI-N1ICD

    Plasmid

    #185525

    Purpose

    Constitutive overexpression of human NOTCH1 intracellular domain

    Depositor

    Sean Palecek, Eric Shusta

    Article

    Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

    Insert

    NOTCH1 intracellular domain (NOTCH1 Human)
    

    Use

    Lentiviral

    Promoter

    EF-1a

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. human beta-catenin pcDNA3

    Plasmid

    #16828

    Purpose

    Mammalian expression of human beta-catenin with Flag tag

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAV-U6+27-Tornado-Broccoli

    Plasmid

    #124360

    Purpose

    Overexpression of circular RNA (Broccoli)

    Depositor

    Samie Jaffrey

    Article

    Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

    Insert

    Tornado-Broccoli
    

    Expression

    Mammalian

    Promoter

    U6+27

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTS117 His14-Avi-45xGS-anti GFP nanobody

    Plasmid

    #199370

    Purpose

    Bacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-GFP nanobody
    

    Tags

    14xHis-Avi

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T5-LacO

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLVX-SRRM2-mCherry

    Plasmid

    #174089

    Purpose

    Inducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on system

    Depositor

    Katie Ullman, Wesley Sundquist

    Article

    Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

    Insert

    SRRM2 (SRRM2 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TREtight

    Available Since

    Sept. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GalT-RpHLuorin2

    Plasmid

    #171719

    Purpose

    Trans-Golgi expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)
    

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGEX6P1-GFP-Nanobody

    Plasmid

    #61838

    Purpose

    Expresses GST-tagged anti-GFP Nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    GFP-Nanobody
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET-NLS-Cas9-6xHis

    Plasmid

    #62934

    Purpose

    Expression of NLS-Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    NLS-Cas9-6xHis
    

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pWM_12x601_35bpLinker

    Plasmid

    #157790

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_35bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge