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Showing: 3451 - 3500 of 4899 results

  1. pADH118-2

    Plasmid
    #90984
    Purpose
    NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. albicans LEU2 2 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90984-plasmid-map-sequence-id-180512

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  2. pJAID5VY

    Plasmid
    #165048
    Purpose
    Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.
    Depositor
    Claudia Vickers
    Article
    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:
    Insert
    P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    OsTIR1 is codon-optimised for expression in S. ce…
    Promoter
    Available since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    165048-plasmid-map-sequence-id-327148

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  3. pML2(−)WF

    Plasmid
    #108337
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108337-plasmid-map-sequence-id-209244

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  8. AP1297‐1

    Plasmid
    #87247
    Purpose
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
    Depositor
    Geraldine Seydoux
    Article
    Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12
    Insert
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
    Use
    Tags
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    87247-plasmid-map-sequence-id-173228

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  9. pJBL712

    Plasmid
    #140382
    Purpose
    Expresses OtrR-regulated 3WJdB RNA aptamer driven by T7 promoter with the otrO sequence 5BP downstream from the promoter
    Depositor
    Julius Lucks
    Article
    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038
    Insert
    T7-otrO-3WJdB-T
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140382-plasmid-map-sequence-id-297931

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  10. pFH71

    Plasmid
    #128214
    Purpose
    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
    Depositor
    Vladimir Nekrasov
    Article
    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d
    Insert
    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128214-plasmid-map-sequence-id-252897

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  12. pML2(+)BR

    Plasmid
    #108336
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    108336-plasmid-map-sequence-id-214578

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  13. pML2(+)WF

    Plasmid
    #108333
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108333-plasmid-map-sequence-id-209522

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  14. pML2(+)BF

    Plasmid
    #108334
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108334-plasmid-map-sequence-id-209526

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  15. pML2(+)WR

    Plasmid
    #108335
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108335-plasmid-map-sequence-id-209243

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  16. pML2(−)BF

    Plasmid
    #108338
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108338-plasmid-map-sequence-id-209524

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  17. pML2(−)WR

    Plasmid
    #108339
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108339-plasmid-map-sequence-id-209534

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  18. pML2(−)BR

    Plasmid
    #108340
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108340-plasmid-map-sequence-id-209245

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  26. pADH118-17

    Plasmid
    #91727
    Purpose
    NAT-marked C. albicans URA3-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    NAT 2of2, pSNR52, C. albicans URA3-specific gRNA, LEU2 2of2
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    91727-plasmid-map-sequence-id-180525

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  27. pADH143-2

    Plasmid
    #90990
    Purpose
    NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. maltosa LEU2 2 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90990-plasmid-map-sequence-id-180523

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  28. pADH100-2

    Plasmid
    #90981
    Purpose
    NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, FRT, DS_HIS1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90981-plasmid-map-sequence-id-180508

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  29. pGS1044

    Plasmid
    #63797
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal 3xFLAG tag and a LEU2 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1
    Insert
    3xFLAG followed by KlLEU2
    Use
    E. coli cloning vector
    Tags
    3xFLAG
    Expression
    Mutation
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63797-plasmid-map-sequence-id-110117

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  31. EcoHIV-mScarlet

    Plasmid
    #235598
    Purpose
    EcoHIV-mScarlet is a modification of pNL4-3-EcoHIV (Potash et al, PNAS 2005). EcoHIV is rodent-infectable modification of HIV where HIV env replaced for MLV-env
    Depositor
    Michael Shtutman
    Article
    Li et al J Vis Exp. 2024 Dec 20;(214). doi: 10.37
    Insert
    NL4-3 EcoHIV mScarlet
    Use
    Lentiviral
    Tags
    mScarlet
    Expression
    Mutation
    Promoter
    Available since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    235598-plasmid-map-sequence-id-469982

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  35. pMDW108

    Plasmid
    #227194
    Purpose
    Template for multi-nucleosome array
    Depositor
    Michelle Wang
    Article
    Le et al Cell. 2019 Oct 17;179(3):619-631.e15. do
    Insert
    64mer array of p601 with 50bp flanking naked DNA between the anchor points and the first and the last 601.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Plac
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    227194-plasmid-map-sequence-id-461742

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  38. pHBJT002

    Plasmid
    #225153
    Purpose
    High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    FLAG epitope tag (35 bp)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    225153-plasmid-map-sequence-id-443288

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Showing: 3451 - 3500 of 4899 results