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Showing: 3501 - 3550 of 32215 results

  3. ScFv-2ERT2-VPH

    Plasmid
    #120556
    Purpose
    Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to 2 tandem ERT2, VP64, P65 and HSF1
    Depositor
    Yu Wang
    Article
    Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25
    Insert
    scFvGCN4, GB1, ERT2, VP64, P65 and HSF1
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    120556-plasmid-map-sequence-id-252529

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  5. CMV-mEGFP(A206K)-paCaMKII(T286A)

    Plasmid
    #165439
    Purpose
    Expresses photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.
    Depositor
    Hideji Murakoshi
    Article
    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1
    Insert
    mEGFP(A206K)-paCaMKII(T286A)
    Use
    Tags
    mEGFP(A206K)
    Expression
    Mammalian
    Mutation
    T286A: Autophosphorylation deficient mutation
    Promoter
    CMV
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    165439-plasmid-map-sequence-id-325972

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  7. pET22b_EcSSB_2

    Plasmid
    #78204
    Purpose
    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor
    Depositor
    Martin Webb
    Article
    Fili et al Nucleic Acids Res. 2010 Jul;38(13):4448-
    Insert
    ssb (W88C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    W88C (please see depositor comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78204-plasmid-map-sequence-id-151737

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  10. LBR-FKBP-GFP

    Plasmid
    #222413
    Purpose
    To express LBR with C-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells
    Depositor
    Stephen Royle
    Article
    Downie et al bioRxiv 2024.05.31.596797
    Insert
    LBR (LBR Human)
    Use
    Tags
    FKBP-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222413-plasmid-map-sequence-id-439134

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  15. c3GIC9n

    Plasmid
    #62192
    Purpose
    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes
    Depositor
    Lukas Dow
    Article
    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do
    Insert
    humanized S. Pyogenes Cas9 (D10A)
    Use
    CRISPR
    Tags
    HA
    Expression
    Mutation
    D10A substitution (Cas9n)
    Promoter
    TRE3G
    Available since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    62192-plasmid-map-sequence-id-156364

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  16. pdUA-lacIN

    Plasmid
    #141079
    Purpose
    Plasmid vector for testing DNA binding proteins by the I-Block assay.
    Depositor
    Antal Kiss
    Article
    Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28
    Type
    Empty backbone
    Use
    Vector for the i-block assay designed to study dn…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Escherichia coli lacIN promoter
    Available since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    141079-plasmid-map-sequence-id-310860

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  39. pGen2.1-redCALWY-4

    Plasmid
    #50738
    Purpose
    Expresses redCALWY-4 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.
    Depositor
    Maarten Merkx
    Article
    Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.
    Insert
    gene for redCALWY-4 Zn2+ sensor
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50738-plasmid-map-sequence-id-76087

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  48. pGen2.1-redCALWY-1

    Plasmid
    #50739
    Purpose
    Expresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.
    Depositor
    Maarten Merkx
    Article
    Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.
    Insert
    gene for redCALWY-1 Zn2+ sensor
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    50739-plasmid-map-sequence-id-76088

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Showing: 3501 - 3550 of 32215 results