We narrowed to 15,661 results for: genome
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Plasmid#58769PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET8
Plasmid#107439PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-hsp70
Plasmid#71500PurposeflySTARR-seq screening vector with hsp70 core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME-luc2
Plasmid#138355PurposeGateway compatible middle entry clone contains Firefly Luciferase2 without minimal promoterDepositorInsertluciferase
ExpressionMammalianAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDB4281
Plasmid#98700PurposeA Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX systemDepositorInsertCas9
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x7
Plasmid#58771PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKDR-CBh
Plasmid#183557PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated CBh promoter)DepositorInsertUnattenuated
ExpressionMammalianPromoterCBhAvailable SinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pREPK219H
Plasmid#51492PurposepREP with a mutation in the ATPase domain (K219H). This is predicted to result in loss of geminivirus replication.DepositorInsertRep/RepA
UsePlant expression t-dnaMutationchanged lysine 219 to histidinePromoter2x35SAvailable SinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
BSP605
Plasmid#122250Purposeexpression vector for mCerulean under EFT3 promoterDepositorInsertmCerulean
ExpressionWormPromoterEFT3Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR1056
Plasmid#71824PurposeExpress Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
UseCRISPRAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-beta2 TKIT donor
Plasmid#169436PurposeDonor DNA for TKIT knockin of SEP to Gabrb2 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRExpressionMammalianAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX458_CEBPB_1
Plasmid#64036PurposeExpresses gRNA against human CEBPB along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRPromoterU6Available SinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC1876
Plasmid#158722PurposeHelper plasmid pYC1640 for C-terminal tagging with degradation tag ssrADepositorInsertssrA
UseCRISPRExpressionBacterialAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX458_CEBPB_2
Plasmid#64047PurposeExpresses gRNA against human CEBPB along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRPromoterU6Available SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSN444
Plasmid#108331PurposeExpresses Sat2 for Nourseothricin resistance selection of C. elegansDepositorInsertStreptothricin acetyl transferase 2
ExpressionWormPromoterrps-27 promoterAvailable SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDU1
Plasmid#169817PurposepSpCas9(BB)-2A-tCD19 (pDU1)DepositorInserttCD19
Tagsfusion proteinExpressionMammalianPromoterCbhAvailable SinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cip-pAct1-Mnase-SAT1
Plasmid#162557PurposeFor the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1DepositorInsert3FLAG-Mnase-SV40
ExpressionYeastPromoterActineAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ148
Plasmid#69300PurposeGolden Gate recipient and Gateway entry vector; assembly of 8 gRNAsDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD-6xHis-BglII
Plasmid#196654PurposeDerived from pGLO (BioRad) by the insertion of a 6xHis Tag and a BglII siteDepositorTypeEmpty backboneTags6xHis tagExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBA787
Plasmid#186242Purposesoc-C HR Donor Vector (T4 phage editing)DepositorInsertBBR1 backbone + T4 phage homology
ExpressionBacterialAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTaU6-esgRNA
Plasmid#115630Purposeexpression esgRNA in wheat protoplastsDepositorInsertTaU6p-esgRNA
UseCRISPRAvailable SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1-PP7-boxB
Plasmid#75393PurposesgRNA1-PP7-boxBDepositorInsertsgRNA1-2XPP7-boxB
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterU6Available SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAEF5
Plasmid#136305PurposePlasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin FleissDepositorTypeEmpty backboneUseCRISPRAvailable SinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP S2TAG
Plasmid#68296PurposeS2TAG GFP reporterDepositorInsertGFP S2TAG
TagsHisExpressionBacterialAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-SAT1
Plasmid#162465PurposepFA-MNase-CaURA3 was double digested to remove the URA3 auxotrophy marker. The SAT1 marker was amplified from pFA-SAT1 and cloned into the AscI-SacI-digested pFA-MNaseDepositorInsert3FLAG-Mnase
ExpressionYeastAvailable SinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluA3 TKIT donor
Plasmid#169425PurposeDonor DNA for TKIT knockin of SEP to GluA3 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRExpressionMammalianAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSfi-HspLacZ
Plasmid#33351DepositorInsertHsp68 promoter (Hspa1a Mouse)
UseMouse TargetingTagsLacZExpressionMammalianPromoterHsp68 (Hspa1a)Available SinceJan. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet26
Plasmid#107448PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet16
Plasmid#107452PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
iGFP
Plasmid#66589Purposeinverted GFP reporterDepositorInsertGFP
PromoterCMVAvailable SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
EZH2_N_FS_v2_Donor
Plasmid#79901PurposeN-terminal tagging of human EZH2 with 3xFLAG_Twin_StrepDepositorTypeEmpty backboneUseCRISPR and TALENTags3xFLAG_Twin_StrepAvailable SinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_PA-Cre-Y324F
Plasmid#122961PurposeCatalytically inactive mutant of PA-CreDepositorInsertpcDNA3.1_PA-Cre-Y324F
UseCre/LoxExpressionMammalianPromoterCMVAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJY3919
Plasmid#113332PurposeHermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoterDepositorInsertsbeta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase
ExpressionYeastAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
PL-452 N-EGFP
Plasmid#19173DepositorInsertEGFP
UseCre/LoxAvailable SinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
BSP611
Plasmid#122256Purposeexpression vector for mtagRFP-T under EFT3 promoterDepositorInsertmtagRFP-T
ExpressionWormPromoterEFT3Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEVA231-CRISPR
Plasmid#138712PurposePlasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR ArrayDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGb_PMLlxP_EGFP
Plasmid#140283Purposea targeting plasmid for EGFP-PMLDepositorInsertEGFP
UseMouse TargetingPromoterendogenousAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP S2TAG/E17TAG
Plasmid#68298PurposeS2TAG/E17TAG GFP reporterDepositorInsertGFP S2TAG/E17TAG
TagsHisExpressionBacterialAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICSL50005
Plasmid#117536PurposeLevel0 Golden Gate part: c-terminal tag, YFPDepositorInsertc-term tag YFP
UseSynthetic BiologyMutationG65S, L68V, A72S, Y203T, L231HAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only