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pGOAL17

Plasmid

#20189

Depositor

Tanya Parish

Article

Parish et al Microbiology (Reading). 2000 Aug;146 ( P

Type

Empty backbone

Use

Marker cassette

Available Since

May 22, 2009

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-RiboL1-jGCaMP8s

Plasmid

#169247

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Laconic/pcDNA3.1(-)

Plasmid

#44238

Purpose

a genetically-encoded sensor for lactate concentration, transport and metabolic flux

Depositor

Luis Felipe Barros

Article

San Martin et al PLoS One. 2013;8(2):e57712. doi: 10.1371

Insert

Laconic (LACtate Optical Nano Indicator from CECs)

Use

Lactate biosensor

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

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K44A HA-dynamin 1 pcDNA3.1

Plasmid

#34683

Depositor

Sandra Schmid

Insert

dynamin 1ba (DNM1L Human)

Tags

HA (variant)

Expression

Mammalian

Mutation

K44A

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pLENTI6.3/AR-GC-E2325

Plasmid

#85128

Purpose

exogenous AR expression

Depositor

Karl-Henning Kalland

Article

Olsen et al BMC Cancer. 2016 Jul 4;16:377. doi: 10.1

Insert

androgen receptor (AR Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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CaVα2δ1

Plasmid

#26575

Depositor

Diane Lipscombe

Article

Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

Insert

Cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W

Plasmid

#67982

Purpose

Cas9 activity reporter with mCherry and GFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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p-EGFP-C1-Flag-mFmr1(wt)

Plasmid

#87929

Purpose

Expresses mouse FMRP-EGFP fusion, which can be phosphorylated on ser-499.

Depositor

Stephanie Ceman

Article

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Insert

Fmr1 (Fmr1 Mouse)

Tags

Flag and eGFP

Expression

Mammalian

Mutation

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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RFP-zyxin

Plasmid

#26720

Depositor

Anna Huttenlocher

Article

Bhatt et al J Cell Sci. 2002 Sep 1. 115(Pt 17):3415-

Insert

Zyxin (ZYX Human)

Tags

DsRed

Expression

Bacterial and Mammalian

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

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pRSETa mEos2

Plasmid

#20341

Purpose

bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

Depositor

Loren Looger

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

mEos-2

Tags

His

Expression

Bacterial

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pINDUCER21 (ORF-EG)

Plasmid

#46948

Purpose

Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

Depositor

Stephen Elledge, Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

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COVID-SARS2 NSP13

Plasmid

#159614

Purpose

Bacterial expression plasmid for COVID-SARS2 NSP13 helicase

Depositor

Opher Gileadi

Article

Newman et al Nat Commun. 2021 Aug 11;12(1):4848. doi:

Insert

Covid-SARS2 Nsp13

Tags

His6-Zb-TEV

Expression

Bacterial

Mutation

Codon-optimized for E. coli expression

Promoter

T7 - LacO

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-E

Plasmid

#78852

Purpose

Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

Depositor

Phillip Abbosh

Article

Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Cas9-P2A-Puro

Expression

Mammalian

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST51-LRRK2-WT

Plasmid

#25080

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Gateway Cloning

Tags

6XHis and V5

Expression

Mammalian

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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PLJR965

Plasmid

#115163

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

Depositor

Sarah Fortune

Article

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR

Use

Unspecified

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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GAG-CRErec

Plasmid

#119971

Purpose

Expresses GAG (FMLV) fused with CRE recombinase for the production of VLPs loaded with CRE protein

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Insert

GAG-nlsCRErec

Tags

nls

Expression

Mammalian

Promoter

hCMV

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ash2L full

Plasmid

#15548

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

Ash2L (ASH2L Human)

Expression

Mammalian

Mutation

R622H (Please see depositor comments below)

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only

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mClY-N1

Plasmid

#90457

Purpose

A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

Depositor

Joseph Santos-Sacchi

Article

Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

Insert

mClY-N1

Expression

Mammalian

Mutation

EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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p37-2iDMD-LR

Plasmid

#88892

Purpose

37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.

Depositor

Michele Calos

Article

Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

Inserts

Dystrophin (DMD Human)
luciferase
mCherry

Expression

Mammalian

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMA4008

Plasmid

#70109

Purpose

mtDNA destruction/production of rho-0 cells

Depositor

Mikhail Alexeyev

Article

Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

Inserts

EGFP
Herpes simplex virus UL12.5M185

Tags

none

Expression

Mammalian

Mutation

N-terminal deletion of UL12.5

Promoter

CMV and RSV

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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Mm-PylRS-AF/Pyl-tRNACUA

Plasmid

#122650

Purpose

tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.

Depositor

Howard Hang

Article

Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

Insert

PylRS-AF, Pyl-tRNACUA

Tags

FLAG

Expression

Mammalian

Mutation

Tyrosine 306 changed to Alanine, Tyrosine 384 cha…

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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AP2u2-mCherry

Plasmid

#27672

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Ap2m1 (Ap2m1 Rat)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42-T17N

Plasmid

#12976

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Cdc42 DN (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T17N Dominant negative

Promoter

CMV

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 myc CIITA (P#808)

Plasmid

#14650

Depositor

Matija Peterlin

Article

Kanazawa et al Immunity. 2000 Jan . 12(1):61-70.

Insert

CIITA (CIITA Human)

Tags

Myc

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-lyso-pHluorin

Plasmid

#70113

Purpose

Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHluorin (Cd63 Aequorea victoria, Mouse)

Tags

HA-Tag and pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pScaffold-H1 donor

Plasmid

#118152

Purpose

PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Type

Empty backbone

Expression

Mammalian

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER13 miR-200c-141

Plasmid

#81020

Purpose

Lentiviral vector containing doxycycline inducible human miR-200c. Puromycin selectable.

Depositor

Jeffrey Rosen, Thomas Westbrook

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

miR-200c (MIR200C Human)

Use

Lentiviral

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB-cLUC

Plasmid

#174051

Purpose

Gateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assay

Depositor

Alberto Macho

Article

Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

Type

Empty backbone

Use

Luciferase

Expression

Plant

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIP403

Plasmid

#122028

Purpose

Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

Depositor

Lars Axelsson, Geir Mathiesen

Article

Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

Insert

gusA

Expression

Bacterial

Promoter

sppA

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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HA GSK3 beta wt pcDNA3

Plasmid

#14753

Purpose

Mammalian expression of human GSK3 beta with HA tag

Depositor

Jim Woodgett

Article

He et al Nature. 1995 Apr 13. 374(6523):617-22.

Insert

GSK3 beta (GSK3B Human)

Tags

HA

Expression

Mammalian

Available Since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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8,964 results