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163,606 results

Showing: 2401 - 2430 of 163606 results

  3. pFD116

    Plasmid
    #124769
    Purpose
    pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Article
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Inserts
    Ptet
    dcas9
    PpflB sgRNA BsaI
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124769-plasmid-map-sequence-id-246181

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  7. pTCF156 HLA-A*02:01

    Plasmid
    #180456
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*02:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available Since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180456-plasmid-map-sequence-id-355799

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  8. AICSDP-15:ACTB-mEGFP

    Plasmid
    #87425
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    ACTB Homology Arms with mEGFP-linker (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available Since
    March 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87425-plasmid-map-sequence-id-174163

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  11. pAAV-hsyn-NE2h

    Plasmid
    #208687
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neurons
    Depositor
    Yulong Li
    Has Service
    AAV5
    Insert
    GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available Since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    208687-plasmid-map-sequence-id-412174

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  14. p3E_he1a:mCherry

    Plasmid
    #113881
    Purpose
    p3E entry vector for Three Multisite Gateway
    Depositor
    Marnie Halpern
    Insert
    he1a (he1.1 Zebrafish)
    Use
    Zebrafish danio expression
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    113881-plasmid-map-sequence-id-223633

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  16. pCW57-MCS1-P2A-MCS2 (Hygro)

    Plasmid
    #80922
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80922-plasmid-map-sequence-id-258301

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  19. pCRISPomyces-2

    Plasmid
    #61737
    Purpose
    Streptomyces expression of codon-optimized Cas9 and custom gRNA
    Depositor
    Huimin Zhao
    Article
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    gRNA cassette
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL) and rpsL(XC)-BbsI
    Available Since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    61737-plasmid-map-sequence-id-190162

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  22. CLYBL-TO-hNIL-BSD-mApple

    Plasmid
    #124230
    Purpose
    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.
    Depositor
    Michael Ward
    Insert
    NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    124230-plasmid-map-sequence-id-240604

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  23. mKeima-Red-Mito-7

    Plasmid
    #56018
    Purpose
    Localization: Mitochondria, Excitation: 440, Emission: 620
    Depositor
    Michael Davidson
    Insert
    Mito (COX8A Human)
    Use
    Tags
    mKeima-Red
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    56018-plasmid-map-sequence-id-201345

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  26. pIVTRup

    Plasmid
    #101362
    Purpose
    Highly efficient in vitro transcription. Empty backbone.
    Depositor
    Ángel Raya
    Insert
    5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
    Use
    For cloning a gene for in vitro transcription pur…
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    101362-plasmid-map-sequence-id-352564

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  28. pSLQ10530

    Plasmid
    #239266
    Purpose
    Expresses NES-dCas13b-ABI for CRISPR-TO perturbation in the cytoplasm of cell lines
    Depositor
    Stanley Qi
    Article
    Han et al Nature. 2025 Jul;643(8070):241-251. doi:
    Insert
    NES-dPspCas13b-EGFP-ABI
    Use
    CRISPR and Lentiviral
    Tags
    Flag tag and V5 tag
    Expression
    Mammalian
    Mutation
    H133A and H1058A in PspCas13b
    Promoter
    EF1a
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239266-plasmid-map-sequence-id-474747

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Showing: 2401 - 2430 of 163606 results