166,806 results

1. pCDH-EF1a-eFFly-mCherry

   Plasmid

   #104833

   Purpose

   3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

   Depositor

   Irmela Jeremias

   Article

   Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

   Inserts

   eFFly
   mCherry
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWN_U6-TRAC-miniGag-Cas9

   Plasmid

   #228959

   Purpose

   miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag-Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYES-mtGFP

   Plasmid

   #45053

   Depositor

   Benedikt Westermann

   Article

   Westermann et al Yeast. 2000 Nov;16(15):1421-7.

   Insert

   mtGFP
   

   Expression

   Yeast

   Promoter

   GAL

   Available Since

   May 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pInducer10b-EGFP-KRAS G12V

   Plasmid

   #164925

   Purpose

   Expresses EGFP cDNA and KRAS G12V cDNA in mammalian cells

   Depositor

   Ji Luo

   Article

   Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

   Inserts

   Enhanced green fluorescent protein
   KRAS proto-oncogene, GTPase (KRAS) G12V mutant (KRAS Human)
   

   Use

   Lentiviral

   Mutation

   Glycine 12 changed to valine (G->T 3156)

   Promoter

   Ubc Promoter and Ubc promoter

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. DYRK1A_pCSdest

   Plasmid

   #53791

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   DYRK1A (DYRK1A Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   Sept. 8, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. EGFR-FR

   Plasmid

   #179263

   Purpose

   Human EGFR C-terminally labeled with FusionRed

   Depositor

   Jared Toettcher

   Article

   Farahani et al Cell Rep. 2021 Dec 28;37(13):110181. doi

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Tags

   FusionRed

   Promoter

   SFFV

   Available Since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. HaloTag-LaminA

   Plasmid

   #231894

   Purpose

   Expression of HaloTag-tagged LaminA to visualize the nuclear lamina

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   LaminA (LMNA Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV GFP1-9

   Plasmid

   #182244

   Purpose

   Tripartite split-GFP mammalian. Express GFP1-9 detector fragment

   Depositor

   Stéphanie Cabantous

   Article

   Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

   Insert

   GFP 1-9 OPT
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRcCMV Cep215 (Nigg CW493)

   Plasmid

   #41152

   Depositor

   Erich Nigg

   Article

   Graser et al J Cell Sci. 2007 Dec 15;120(Pt 24):4321-

   Insert

   Cep215 (CDK5RAP2 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRetro-CAG-DsRedExpress-TVA

    Plasmid

    #235699

    Purpose

    Control plasmid expressing TVA and DsRed without G protein

    Depositor

    Guo-li Ming, Hongjun Song

    Article

    Sun et al Nature. 2025 Jan 16. doi: 10.1038/s41586

    Insert

    DsRedExpress, TVA800
    

    Use

    Retroviral

    Promoter

    CAG

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-Puro_hCOL4A4-Myc-His

    Plasmid

    #198093

    Purpose

    Express hCOL4A4 in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    COL4A4 (COL4A4 Human)
    

    Tags

    TEV-Myc-His

    Expression

    Mammalian

    Promoter

    CMV, beta-globin/IgG chimeric intron

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pChillyBoys_gen1

    Plasmid

    #228495

    Purpose

    pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)

    Depositor

    Richard Baker

    Inserts

    groEL
    groES
    

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pClneoMyc-LATS1

    Plasmid

    #66851

    Purpose

    Express Myc-tagged LATS1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    LATS1 (LATS1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P44L mutation in LATS1 compared to GenBank refere…

    Promoter

    CMV

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

    Plasmid

    #187334

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PGK-DTAG-CTERM-GFP

    Plasmid

    #185770

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, dTAG

    Mutation

    none

    Promoter

    PGK

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPRv2_mSTING_scrambled_gRNA_1

    Plasmid

    #196627

    Purpose

    Scrambled control 1 for STING knock-out in murine cells.

    Depositor

    Nicolas Manel

    Article

    Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

    Insert

    mSTING scrambled gRNA 1
    

    Use

    CRISPR and Lentiviral

    Mutation

    WT

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. gTEMP_pcDNA3

    Plasmid

    #89583

    Purpose

    Genetically encoded ratiometric fluorescent thermometer

    Depositor

    Takeharu Nagai

    Article

    Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

    Insert

    gTEMP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pH6HTC_ENO1-Halo

    Plasmid

    #175330

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    ENO1 (ENO1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3.1-Myoferlin HA

    Plasmid

    #22443

    Depositor

    William Sessa

    Article

    Bernatchez et al J Biol Chem. 2007 Oct 19. 282(42):30745-

    Insert

    Myoferlin
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF2538

    Plasmid

    #144036

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX4 (SOX4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pT7-hMLH1dn for IVT

    Plasmid

    #178114

    Purpose

    Template for in vitro transcription of human MLH1dn

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized) (MLH1 Human)
    

    Use

    Template for in vitro transcription

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-EFS-DIO-GRAB_Ado1.0

    Plasmid

    #153291

    Purpose

    Express the adenosine sensor GRAB_Ado1.0 in Cre positive cells

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine sensor GRAB_Ado1.0
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3-LacR-TopBP1

    Plasmid

    #31317

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

    Insert

    TopBP1 (TOPBP1 Human)
    

    Tags

    FLAG and LacR

    Expression

    Mammalian

    Available Since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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24. pWUR790

    Plasmid

    #101723

    Purpose

    Synthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgo

    Depositor

    John van der Oost

    Article

    Swarts et al Nucleic Acids Res. 2015 May 26;43(10):51

    Insert

    PfAgo
    

    Tags

    Strep(II)-tag

    Expression

    Bacterial

    Promoter

    T7 Promotor

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pC0043-PspCas13b crRNA backbone

    Plasmid

    #103854

    Purpose

    For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6-BbsI-BbsI-PspCas13b DR-polyT
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 28, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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26. pCRIS-PITChv2-dTAG-Puro (BRD4)

    Plasmid

    #91796

    Purpose

    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    FKBP_F36V-2xHA-P2A-Puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pDONR221_SLC16A3

    Plasmid

    #131899

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC16A3 (SLC16A3 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pTKEI-Tre-C04

    Plasmid

    #79754

    Purpose

    Single fluorescent protein biosensor for trehalose

    Depositor

    David Savage

    Article

    Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

    Insert

    Tre-C04
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    pTrc

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. pAAV-hSyn-DIO-GRAB_ACh3.0

    Plasmid

    #121923

    Purpose

    express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

    Insert

    GPCR activation based ACh sensor GRAB-ACh3.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. miReporter-CAG

    Plasmid

    #82478

    Purpose

    microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

    Insert

    H2B-mCherry and H2B-Citrine. HygroR
    

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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