19,178 results

1. pHR-DDX4N-mCh-Cry2WT

   Plasmid

   #101225

   Purpose

   DDX4(1-236) fused to mCh-Cry2WT

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   DDX4(1-236)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Flag-NPM1wt

   Plasmid

   #131810

   Purpose

   Expresses Flag-NPM1wildtype (human) in mammalian cells through transient transfection

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUbC-OsTIR1-myc-IRES-scFv-sfGFP

   Plasmid

   #84563

   Purpose

   Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP

   Depositor

   Robert Singer

   Article

   Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

   Insert

   Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
   

   Use

   Lentiviral

   Tags

   myc tag (9x)

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSNAPf-hYTHDF1

   Plasmid

   #155346

   Purpose

   Express full-length human YTHDF1 with a C-terminal SNAP-tag in mammalian cells

   Depositor

   Xiaowei Zhuang

   Article

   Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

   Insert

   YTHDF1 (YTHDF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO-Tet-On-shRNA-Control

   Plasmid

   #98398

   Purpose

   Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   non-targeting randomized sequence
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE

   Plasmid

   #60906

   Purpose

   The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imaging

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLIX402 PHF2

   Plasmid

   #91700

   Purpose

   inducible lentiviral expression of PHF2

   Depositor

   Bob Weinberg

   Article

   Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

   Insert

   PHF2 (PHF2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV JMJD3 mutant

   Plasmid

   #21214

   Depositor

   Paul Khavari

   Article

   Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

   Insert

   JMJD3 (KDM6B Human)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   H1390A

   Promoter

   Available since

   June 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-ZO1-C-14

   Plasmid

   #55165

   Purpose

   Localization: Tight Junctions, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   ZO1 (TJP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. hCas9

    Plasmid

    #41815

    Purpose

    Expresses human codon optimized Cas9 nuclease for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-EF1a-EGFP-ERGIC53-IRES-Puromycin

    Plasmid

    #134859

    Purpose

    Expresses EGFP-tagged ERGIC marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    ERGIC53 (Lman1 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. LeGO-G2

    Plasmid

    #25917

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. Artichoke

    Plasmid

    #73320

    Purpose

    Artichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

    Depositor

    Benjamin Ebert

    Article

    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and EF1a

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-vCreDIO hChR2(H134R)-EYFP

    Plasmid

    #55643

    Purpose

    vCre-Dependent ChR2-EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    hChR2(H134R)-EYFP
    

    Use

    AAV; Vcre-dependent chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTriEx-mCherry-Zdk1

    Plasmid

    #81057

    Purpose

    mammalian expression of Zdk1

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Use

    Tags

    6x His tag, FLAG, and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCasKP-hph

    Plasmid

    #117232

    Purpose

    Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-T7pol

    Plasmid

    #59926

    Purpose

    Expression vector for T7 RNA polymerase gene

    Depositor

    Ian Wickersham

    Insert

    T7 RNA polymerase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG:H2B-EGFP

    Plasmid

    #32599

    Depositor

    Virginia Papaioannou, Anna-Katerina Hadjantonakis

    Article

    Hadjantonakis et al BMC Biotechnol. 2004 Dec 24;4:33.

    Insert

    H2B-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

19. ppyCAG_RNaseH1_WKKD

    Plasmid

    #111905

    Purpose

    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids

    Depositor

    Xiang-Dong Fu

    Article

    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

    Insert

    RNASEH1 (RNASEH1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…

    Promoter

    Available since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pW17

    Plasmid

    #158207

    Purpose

    2lox landing pad plasmid with mariner transposon and transposase used for CRAGE

    Depositor

    Yasuo Yoshikuni

    Article

    Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

    Insert

    loxP/Cre/KmR/Lox5171/Mariner IR oriT/transposase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSLIK DN Rac1

    Plasmid

    #84642

    Purpose

    Lentiviral expression of doxycycline-inducible dominant negative Rac1 and co-expression of Venus

    Depositor

    Sanjay Kumar

    Article

    MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Dominant Negative

    Promoter

    Available since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

22. TLCV2-APOBEC1-YTH

    Plasmid

    #178949

    Purpose

    Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

    Depositor

    Kate Meyer

    Article

    Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

    Insert

    APOBEC1-YTH-T2A-GFP
    

    Use

    Lentiviral and Synthetic Biology; Inducible

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLC-Flag-CRBN-P2A-Hygro

    Plasmid

    #124303

    Purpose

    Lentiviral vector for expression of Flag tagged CRBN-P2A-Hygro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    CRBN (CRBN Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138151

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR_BsmBI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. tetO.Sox9.Puro

    Plasmid

    #117269

    Purpose

    Expresses Sox9 and puromycin resistance gene under control of tetO sequence.

    Depositor

    Henrik Ahlenius

    Article

    Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

    Insert

    Sox9 (Sox9 Mouse)
    

    Use

    Lentiviral

    Tags

    T2A and puromycin resistant cassette (C terminal …

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pGL3 basic E2

    Plasmid

    #48747

    Purpose

    Expression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1)

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

    Insert

    mPer2 promoter/enhancer region containing E-box2 (-112 to +98)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCJ136

    Plasmid

    #162665

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLenti6-CENP-A-mCherry

    Plasmid

    #89767

    Purpose

    Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A

    Depositor

    Torsten Wittmann

    Article

    Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

    Insert

    CENP-A (CENPA Human)
    

    Use

    Lentiviral

    Tags

    3xHA and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. OmpA3-PNGaseF-TEV-His6

    Plasmid

    #114274

    Purpose

    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.

    Depositor

    Martin Caffrey

    Insert

    Peptide: N-glycosidase F
    

    Use

    Tags

    OmpA3 leader sequence (for periplasmic localisati…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV G-CEPIA1er

    Plasmid

    #58215

    Purpose

    Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    G-CEPIA1er
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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